Posted by admin on Jun 30, 2017 in |
Curcumin has been shown to inhibit the activation of nuclear factor-κB (NF-κB), which is closely linked to chemo-resistance, in multiple myeloma cells. Malignant cells of this kind typically develop progressive multidrug resistance. Our aim was to investigate new possibilities for overcoming this resistance by suppressing cell signaling pathways. MTT-dye reduction assay was used to evaluate the cytotoxic activity of degrasyn, curcumin and Arsenic (ATO) on U-266, RPMI-8226 cell lines. Carfilzomib served as reference drug. Western blot analysis was performed. In RPMI-8226 cells evident activation of caspase 3 by ATO was seen. PARP cleavage was detected after degrasyn treatment. Curcumin led to PARP degradation as well. Caspase 9 was found to be activated by ATO, degrasyn and Carfilzomib. LC3b (autophagy hallmark) level was increased after exposure to ATO, curcumin and degrasyn. In U-266 cells significant LC3b increase was recorded after carfilzomib treatment. Procaspase 9 was cleaved after curcumin usage. Degrasyn activated procaspase 3. Elisa test was performed. All substances showed inhibition of NF-κB p65 fraction in both U-266 and RPMI-8226...
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Posted by admin on Jun 30, 2017 in |
The present study was conducted to evaluate the antioxidant, antibacterial and nutraceutical properties of three coloured scented rice varieties grown in North Eastern region. The study for the analysis of Nutraceutical content involved more than five contents to find a significant correlation of the all the rice varieties. Antioxidant activity was measured using hydrogen peroxide and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. The antioxidant activities of the studied samples were expressed as percentage (%) of DPPH and H2O2 radicals inhibition and IC50 value. Among different extracts of crude rice, methanolic extract of the black rice showed highest antioxidant activity compared to the aqueous extract of the other two varieties each for DPPH (89.29±0.139) as well H2O2 (80.97±0.091) with an IC50 value of 40 µg/ml and 42.6µg/ml respectively. Also the result of MIC against the tested staphylococcus strain was notably found to be effective with a significant value of 119 µg/ml for black rice taken DMSO as the positive...
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Posted by admin on Jun 30, 2017 in |
The main objective of the present study includes the assessment of nutritional constituents and anti-oxidant activity of methanolic stem extract of Caralluma attenuata by in-vitro methods. In the present investigation the author has identified the phytochemical constituents which have nutritional importance by specific chemical tests, thin layer chromatography, atomic absorption spectroscopy for Calcium, Zinc and Magnesium. In vitro antioxidant property was also performed by Free Radical Scavenging Assay, Reductive Ability and Nitric Oxide Method. Carbohydrates, proteins and amino acids, flavonoids and inorganic essential trace elements were identified and the results were tabulated. On the basis of the results observed the methanolic stem extract of Caralluma attenuata found to possess considerable amounts of nutritional constituents and anti-oxidant...
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Posted by admin on Jun 30, 2017 in |
In this research work Zn1-xAgxO1-δ (where x = 0.05, 0.10, 0.15 and 0.20) nano powders were synthesized via simple soft chemical method using corresponding metal nitrates and alkali precipitant. Sodium hydroxide was used as a precipitant during the synthesis. The prepared samples were characterized using X-ray diffraction, FTIR spectroscopy, particle size analysis, SEM, EDAX analysis and TEM. The antimicrobial activity of the silver doped zinc oxide nanoparticles were studied against both Gram positive (Staphylococcus aureus and Bacillus subtilis) and Gram negative (Escherichia coli and Klebsiella pneumoniae) bacteria by agar well diffusion method. Considering the better antimicrobial properties obtained with Zn0.80Ag0.20O1-δ, these materials were subjected for in vivo acute oral nanotoxicological studies in Swiss Albino mice. The histopathological changes in livers and kidneys of Swiss Albino mice due to the oral ingestion of the above material (Zn0.80Ag0.20O1-δ) at different dosage levels on day 14 administration have shown the LD50 as greater than 2000 mg/kg body...
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Posted by admin on Jun 30, 2017 in |
Objective: Ginger (Zingiber officinale Roscoe), a well-known spice plant, has been used traditionally in the treatment of a wide variety of ailments. The main objective of the work is to evaluate the antidepressant and antinociceptive activity of ethanolic extract of Zingiber officinale in Swiss albino mice. Methods: The present study was design to evaluate the antidepressant property of Zingiber officinale as well as its interaction with conventional antidepressant drug using forced swim test and to evaluate possible mechanism involved in its action. Antinociceptive activity was evaluated by acetic acid induced writhing and its interaction with conventional analgesic drug was also noted. The rhizomes of ginger were authenticated and extraction of dried rhizomes was done by using soxhlet apparatus to obtain its ethanolic extract. Results: The alcoholic extract of Zingiber officinale (300mg/kg p.o and 150mg/kg p.o) for antidepressant activity significantly reduced the immobility time in mice (P<0.05) as compared to the vehicle control. The extract of Zingiber officinale (100 and 200 mg/kg, p.o.) significantly suppressed the acetic acid-induced writhing response...
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