Posted by admin on May 31, 2017 in |
The purpose of the current investigation was to ameliorate the aqueous solubility and dissolution rate of an anti diabetic drug Glimepiride by cyclodextrin ternary complexes employing aminoacids. Initially, Glimepiride (GMP) binary complexes with β-Cyclodextrin (βCD) and Hydroxy propyl β-Cyclodextrin (HPβCD) were formulated by physical mixing, kneading, solvent evaporation and spray drying techniques which was followed by ternary complex preparation of selected GMP-Cyclodextrin binary complex employing various aminoacids by kneading method. The kneading method was used in preparing ternary complexes of GMP, because it was proved to be the best method comparatively in yielding promising binary complexes of glimepiride in the initial stage of this study. GMP formed 1:1 M stoichiometric binary and ternary inclusion complexes as demonstrated by the AL-type of phase solubility curve. An increment in the stability constant value (Kc) of GMP- βCD/HPβCD complex in the presence of aminoacids conceded higher complexation competency. FTIR and DSC studies evidenced the perfect inclusion complex formation. Ternary complexes ameliorated drug dissolution compared with GMP and GMP-βCD. The ternary complex containg...
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Posted by admin on May 31, 2017 in |
The aim of the present study was to evaluate the anxiolytic effects and motor coordination activity of Huperzine-A in rats. Wistar rats were treated orally with Huperzine-A (0.5, 1, 2 & 4 mg/kg) and diazepam (2 mg/kg p.o) as a standard anxiolytic drug then exposed to Elevated plus Maze (EPM) and Light Dark Box (LDB). The effects of the Huperzine-A on motor coordination were evaluated by actophotometer test. The rats treated with Huperzine-A drug (2mg/kg and 4mg/kg) showed significant increase in the percentage of entries and time spent in open arms of EPM, which is thought to reflect anxiolytic-like effects. Furthermore, Huperzine-A extracts (1, 2 & 4 mg/kg) significantly increased the percentage of time spent and number of transitions in the Light box in LDB test, indicating the anxiolytic effects of the substance. This anxiolytic effect of the huperzine-A was comparable to that of the diazepam, taken as standard. Also the Huperzine-A (2 mg/kg and 4 mg/kg) showed significant alteration in motor coordination compared to that of normal control...
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Posted by admin on May 31, 2017 in |
Background: Increase prevalence of viral infection such as hepatitis B or/and hepatitis C are followed by high incidence of mortality among hemodialysis patients. Objective: To determine prevalence and correlation of viral infection (Hepatitis B or/and Hepatitis C) among Hemodialysis Patients that died for the last five years in Indonesia and Malaysia in relation to gender, age, causes, and country. Method: A retrospective study was done in this research. The study included only hepatitis patients (hepatitis B and / or hepatitis C) passed away from 2010 to 2015. Universal sampling was used to select 185 patients from Indonesia hospital and 14 patients in Malaysia hospital but only 108 patients in Indonesia and 5 patients in Malaysia were included in this research. Results: The result indicates that more than 50 % patients have hepatitis C (63 patients). Hepatitis C mostly occurred among male and hypertensive patients between 51-60 years old. There is a significant correlation (P value ≤ 0.05) between age and type of infection for all hemodialysis hepatitis patients. Also,...
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Posted by admin on May 31, 2017 in |
Association rule mining is the recent research area in data mining. Frequent Itemset Mining techniques is one of the prominent techniques in pattern mining. In this system frequent itemset mining is used to find the frequent amino acids patterns in Dengue Virus Type1 data set. This system uses an integration of Transaction Reduction and Random Sampling (TRRS) approach to identify the frequent patterns in Dengue Virus Type1 amino acids sequence. Our system reveals the association between the amino acids. Our System first identifies the number of amino acids sequences suitable for each transaction and finds the number of association rules using Transaction Reduction and Random Sampling methods by varying the sample size. Experimental results show that the performance of our proposed Transaction Reduction and Random Sampling Algorithm(TRRS) works efficiently when compared to Apriori algorithm, FP Growth algorithm, Two Dimensional Transaction Reduction(TDTR) Algorithm Improved TDTR algorithm, Set Oriented Mining (SETM) algorithm and Improved SETM Algorithm (ISETM) in terms of number of association rules generated and the time taken to generate...
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Posted by admin on May 31, 2017 in |
Brassica campestris Linn. (yellow mustard), a common Indian condiment, possesses melatonin as an important antioxidant. Optimization of supercritical carbon dioxide extraction (SC-CO2) process parameters was conducted to obtain an extract having the best combination of yield of melatonin and maximum in vitro hypoglycaemic and hypocholesterolemic activities, along with a desirable balance of ω6 to ω3 fatty acids. A full factorial design and response surface methodology were employed for process optimization. Melatonin content of the extracts was obtained by high performance liquid chromatography and by liquid chromatography-mass spectrometry. The optimized extraction conditions were obtained at 300 bar, 50 °C for 120 min. This extract exhibited highest content of melatonin (660.72±41.05 ng/g of dry mustard powder), maximum in vitro hypocholesterolemic (60.11±3.89%) and hypoglycaemic (59.70±2.67µg/ml) activities and possessed minimum ω6 to ω3 (1.29) ratio with minimum erucic acid content (2.00±0.20 g/100g of fatty acid). The electron paramagnetic resonance spectroscopy of the extract (0.10±0.001 g/ml) showed 72.10±4.01% scavenging activity of DPPH radicals, establishing its strong antioxidant potency. A new Chrastil equation was developed...
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