Posted by admin on Jun 30, 2017 in |
Background and aim: Thymoquinone (TQ), which is the most bioactive component of Nigella sativa (Black cumin), exhibits anticancer characteristics based on cell culture and experimental animal studies. However, molecular action mechanisms of these effects are not clear. MicroRNAs (miRNAs), small non-coding RNAs of approximately 22 nucleotides, are an emerging class of gene expression modulators with relevant roles in several biological processes, including cell differentiation, development, apoptosis, and regulation of the cell cycle. The purpose of this study was to investigate the potential impact of thymoquinone (TQ) on MCF-7 human breast cancer cell miRNAs. Materials and methods: The expression levels of miRNAs in MCF-7 cell and TQ treated MCF-7 cells were estimated by miRNA sequencing. The expressions of miRNAs were determined real-time qPCR. Results: We detected 10 down-regulated mirRNAs (hsa-miR-1, let 7c-5p, hsa-miR-15b-5p, hsa-mir-202-3p, hsa-miR-214-3p, hsa-miR-210-3p, hsa-miR-31-5p, hsa-miR-424-5p, hsa-miR-497-5p, hsa-miR-98-5p) and 2 up regulated miRNAs (hsa-miR-22-3p, hsa-miR-132-3p) in TQ treated groups, comparing with control group. These findings highlight the effects of TQ miRNA profile and molecular mechanism on MCF-7 cells....
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Posted by admin on Jun 30, 2017 in |
The objective of the current study was to differentiate contraceptives for their effects on carbohydrate metabolism. This is a cross sectional design that was conducted in different clinical settings of urban population who belong to low socio economic status by convenience sampling method. Females representing fertile age ranging from 26 to 32 and maintained on different types of hormonal and non hormonal contraceptives (OC; Oral contraceptives, DMPA; Depo-medroxy progesterone, NET-EN; Norethisterone , Implant and non hormonal intrauterine contraceptive device (IUCD) are enrolled and invited in 12 hrs fasting to give their blood samples. Fasting blood sugar is analyzed by GOD–PAP method. All the readings were fed on MS- Excel to evaluate mean ± S.D, Comparative effects were represented by percentile differences between different groups. The present study concluded that all hormonal and non hormonal methods posed no deleterious effects on carbohydrate metabolism. These minimized risks may be due to young age and good monitoring of therapy in clinical practices and could be threatening in the areas where these are...
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Posted by admin on Jun 30, 2017 in |
The challenges of free radicals and tumour promoting agents are on the increase and this informed investigation of the antioxidant property and anticancer potential of Vernonia amygdalina, a most common medicinal vegetable in Nigeria. The leaf of Vernonia amygdalina was extracted in methanol and partitioned into hexane, ethyl acetate and aqueous layers successively. Activity guided fractionation further subjected the active ethyl acetate fraction to vacuum liquid chromatography (VLC). The antioxidant activity of the extracts was measured by the DPPH radical scavenging assay. The anticancer potential of the plant extracts was assessed by the DNA nicking assay and apoptosis induction assay. The sensitivity of the mitochondrial membrane transition pore was determined by studying the rate of change in absorbance at 540 nm under energized and de-energized conditions using Spectrum Lab UV-visible spectrophotometer. The antioxidant activity of the extracts varied considerably with respect to IC50 values. The results proved that V. amygdalina extracts had significant reduction in the formation of nicked DNA but demonstrated increased native form of DNA. The column...
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Posted by admin on Jun 30, 2017 in |
The purpose of this research work is to determine whether Metafol-L improves sensory neuropathy in patient with Type 2 diabetes or not. In patients with diabetes mellitus, prolonged hyperglycemia can result in peripheral neuropathy (pain and/or loss of sensation in the extremities). Neuropathy issues may be related to Vitamin B deficiencies; therefore, the supplementation of Vitamin B12, Vitamin B6, and folate (Vitamin B9) may improve diabetic peripheral neuropathy (DPN). Metafol L is a product containing L-methyl folate, pyridoxal 5 phosphate, and methylcobalamine for management of endothelial dysfunction. Metafol-L ingredients counteract endothelial nitric oxide synthase uncoupling and oxidative stress in vascular endothelium and peripheral nerve. Studies of monotherapy with l-methylfolate, methylcobalamine and pyridoxine 5 phosphate suggest that each of these bioavailable B vitamins may reverse the pathophysiology and symptoms of DPN. After approximately six month of treatment, patient undergoes follow up biopsy. At the end of their treatment 79% of patient showed an increase in calf ENFD & 87% of patient show experienced both reduced frequency and integrity of paraethesias...
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Posted by admin on Jun 30, 2017 in |
To study the analgesic and antipyretic activities of ethanolic extract of Bambusa arundinacea leaves (EEBA) in rats by using models tail immersion test, hot plate test and brewer’s yeast pyrexia model. Methods: The dried powdered leaves were extracted with ethanol and the resultant extract was subjected for phytochemical investigation to identify different phytoconstituents. Analgesic activity of the ethanolic extract of B. arundinacea leaves at a dose of 100mg/kg b.w., p.o. and 200mg/kg b.w., p.o. were evaluated against the standard drug Diclofenac sodium at a dose of 10mg/kg b.w., i.p. by tail immersion test and hot plate test. Antipyretic activity of EEBA (100 mg/kg and 200 mg/kg) was assessed by using brewer’s yeast pyrexia model in rats against the standard drug Paracetamol (150 mg/kg, p.o.). Results: Preliminary phytochemical investigations showed the presence of flavonoids, alkaloids, steroids, carbohydrates, proteins and amino acids, tannins and phenolic compounds as major secondary metabolites. The results showed that in tail immersion and hot plate test, EEBA has shown significant (P < 0.05) increase in the...
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