Posted by admin on Jul 31, 2017 in |
A hydrophilic polysaccharide become isolated from green fruit of Musa paradisiaca L. by the usage of wet milling procedure and distilled water extraction the use of sodium sulphite solution, accompanied by way of maceration, centrifugation and sedimentation. The isolated polysaccharides became characterised for physicochemical properties, viscosity and flow behaviour, scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and XRD study. Diverse concentrations of isolated polysaccharide had been studied for binding property towards Maize starch in traditional tablet formulation. In vivo toxicity study detected secure nature of the isolated polysaccharide. Standard, the outcomes of the present study indicated that isolated polysaccharide is resembles to starch and not useful for frozen products. Polysaccharide is shows Pseudoplastic nature with thixotropic behaviour and smooth surface and elongated oval shape granules seen on SEM Micrograph. Form experiment the optimized concentration banana starch as a binder is 5 to 6 %w/w for solid dosage forms. The outcomes of the study suggested that isolated polysaccharides is promising binding agent as a pharmaceutical excipient in drug delivery...
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Posted by admin on Jul 31, 2017 in |
A sensitive and high throughput high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method has been developed for estimation of methotrexate in human plasma. The sample extraction of methotrexate and methotrexate-d3 (Internal standard) was done from 200 µL of human plasma using solid phase extraction procedure. The chromatographic retention was done on Thermo Butyl Hypersil, 50 x 4.6 mm, 5µm column under isocratic conditions using acetonitrile, 10 mM ammonium formate and 0.5% Formic aid (70:15:15, v/v) as the mobile phase. The mass transition for methotrexate (m/z 455.3 → 308.2), and IS (m/z 458.3 → 311.2) were monitored in the positive ionization mode. The method was validated over a wide-ranging concentration of 0.500-1000 ng/mL. The extraction recovery for the analyte and internal standard was 85.6 %. Stability of methotrexate in plasma was examined under different storage conditions like bench top, wet extract, freeze-thaw and long-term stability. The validated method was used to support a bioequivalence study of 15 mg methotrexate tablet in 16 adult patients. The method reproducibility was proved...
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Posted by admin on Jul 31, 2017 in |
Cyclic adenosine monophosphate (cAMP) dependent protein kinase plays a major role in cell signaling pathway. Over expression of extracellular cAMP dependent protein kinase catalytic subunit caused tumorgenesis in prostate. This protein may provide a base for the design of clinically applicable therapeutic strategies. In order to develop potential inhibitor of cAMP dependent protein kinase catalytic subunit, a high throughput virtual screening of zinc natural compounds database was conducted. Furthermore, QikProp, ADMET predictor and MM-GBSA was performed for ADME (Absorption, Distribution, Metabolism and Elimination), toxicity and binding energy prediction for ligands, respectively. Finally, molecular dynamics simulation was performed to get potential inhibitor. The crystal structure of cAMP dependent protein kinase was used for current study. The entire library of natural compounds was screened using HTVS for the primary level of screening. The binding of the compounds was studied using standard precision followed by Extra Precision algorithm of Glide Docking. On the basis of docking scores top ten hit compounds were selected for further analysis. The Binding affinity was further calculated...
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Posted by admin on Jul 31, 2017 in |
Prunus cornuta (family Rosaceae) commonly known as ‘Himalayan Bird Cherry’, is a deciduous tree growing to 15 m (49ft 3in) at elevations of 2100-3500 meters. It is hardy to zone (Uttarakhand) 5. It is in flower in May, and the seeds ripen from July to October. The flowers are hermaphrodite and are pollinated by insects and birds. It can grow in semi-shade (light woodland) or no shade. It prefers moist soil. Plant extract (sample named PC-1) of Prunus cornuta bark was prepared in methanol and the prepared extract was subjected to Phytochemical screening which shows the presence of steroids, terpenoids, alkaloids, tannin, saponin, carbohydrate, protein etc. The methanolic extract ( sample named PC-1) of powdered bark of Prunus cornuta were examined for their antimicrobial activity using agar well diffusion method against different pathogenic bacterial stains like Staphylococcus aureus, Pseudomonas aeruginosa, Aspergillus niger and Candida albicans. Thus minimum inhibitory concentration(MIC) of resulting extract were also checked. The sensitivity of the pathogens was also checked with standard antibiotics erythromycin, Fluconazole, and...
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Posted by admin on Jul 31, 2017 in |
Members of the tribe Anthemideae (Asteraceae) attract considerable medicinal attention worldwide and are used for the treatment of many infectious and degenerative diseases in humans due to their biologically active compounds. The aim of this study was to evaluate the antioxidant property of total phenolic compounds (TPC) and flavonoids (TF) in the capitula of 41 representatives and endemic taxa belonging to seven genera (Achillea, Anthemis, Artemisia, Glebionis, Matricaria, Tanacetum and Tripleurospermum) native to Turkey. The results of the experiments showed that the phenolics of the taxa were significantly correlated and associated with their antioxidant capacity values. Our findings will be useful to researchers and others who are interested in the antioxidant potentials of the herbs as dietary supplements. This is the first report to describe the phenolic compound and antioxidant capacity values of capitula only in selected taxa of...
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