Posted by admin on May 31, 2017 in |
Objective of the present work was to evaluating the antidiabetic property of Kandelia candel and Rhizophora apiculata extracts by using in vitro assays. The serial exhaustive extraction was carried out with a series of solvents: chloroform, ethyl acetate, methanol, ethanol and water with increasing polarity using Soxhlet apparatus. The concentrated and dried extracts were evaluated for antidiabetic activity by employing standard in vitro techniques (Alpha-amylase and glucose uptake assay using yeast model). In vitro antidiabetic studies show that in case of Kandelia candel methanol extract showed higher antidiabetic activity where as incase of Rhizophora apiculata aqueous extract exhibited significant activity when compared to other solvent extracts. Results confirm that aqueous extract of Rhizophora apiculata exhibited highest antidiabetic activity among all extracts. This study provides scientific evidence that the leaves of Kandelia candel and Rhizophora apiculata have anti-diabetic efficacy. However further comprehensive chemical and pharmacological investigation should be carried out to isolate the active compounds and appropriate elucidation of its mechanism of action and it helps in the development of...
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Posted by admin on May 31, 2017 in |
Dental biofilms are majorly developed by Streptococcus mutans, a cariogenic microorganism and are one of the major effects of glucosyltransferases (Gtf) produced by it. This study was conducted to determine the antagonistic potential of probiotic bacteria against S. mutans biofilm formation. We studied the anti-biofilm formation ability of three Lactobacillus species viz L. rhamnosus, L. acidophilus and L. plantarum. Inhibition of biofilm formation was checked on a cell culture cluster. The samples with robust inhibitory activity were selected to check their effect on Gtf activity by estimating the changes in the amount of glucan synthesized, the Gtf catalyzed reaction product. The results showed that, the cell free broth of L. plantarum and L. acidophilus has significant inhibitory and displacement activity on biofilm formation by the cariogenic organism when employed individually and in combination. Further, the interference (80% reduction) in glucan synthesis observed by applying these lactic acid bacteria (LAB) samples suggested their possible role in inhibition of glucosyltransferase (insoluble) [Gtf-I]. In conclusion, our studies demonstrated repressive activities of two...
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Posted by admin on May 31, 2017 in |
Synthesis of metal nanoparticles has become an important branch of nanotechnology and there is an increasing commercial demand for nanoparticles due to their wide applications. The present study was carried to synthesize silver nanoparticles (AgNPs) using the ethanolic extract of the Leucas aspera and evaluate its in-vitro anti inflammatory activity. The synthesis was done by using the ethanolic solution of L. aspra extract and AgNO3. The AgNPs were characterized using UV-Spectroscopic analysis, Scanning Electron Microscopy (SEM), Fourier Transform Infra Red Spectroscopy (FTIR) analysis. UV-Vis spectral analysis shows a maximum absorption peak at 421.00 nm. The FTIR spectra of AgNPs exhibited prominent peaks at 3697cm-1,1761cm-1,1390cm-1, 831cm-1 which are associated with OH stretching, C=C stretching, CH stretching respectively. The SEM analysis of the synthesized AgNPs clearly showed the clustered and irregular shapes, mostly aggregated and having the size of 25-80 nm. The synthesized AgNPs exhibited an enhanced anti inflammatory activity due to the synergistic effect of phytoconstituents in L. aspera extract and the silver...
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Posted by admin on May 31, 2017 in |
In the present study, an attempt was made to design and evaluate mucoadhesive bilayer tablet of losartan potassium (angiotensin II receptor antagonist), in order to overcome bioavailability problems, to reduce dose dependent side effects and frequency of administration. The losartan potassium mucoadhesive buccal tablet were prepared by wet granulation method using chitosan as a mucoadhesive polymer to prolong the drug release and to avoid first pass metabolism. The prepared Mucoadhesive buccal tablets were evaluated by pre and post compression parameter. Precompression test like bulk density, tap density, angle of repose and post compression test include thickness, hardness, friability, weight variation, drug content, disintegration time, wetting time, surface pH, in-vitro drug release and ex-vivo mucoadhesive time. The drug excipients compatability was evaluated by DSC...
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Posted by admin on May 31, 2017 in |
Cholesterol is a waxy substance which is made in the body by the liver but is also found in some foods. It plays a vital role in how every cell works and is also needed to make Vitamin D, some hormones and bile for digestion. The concept of energy of a graph was introduced by I. Gutman in the year 1978. In this paper, we compute Siedel Energy, Maximum Degree Energy, Distance Energy, Harary Energy of...
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