Posted by admin on Jan 31, 2024 in |
Background: In this study, Embelia ribes fruit extract phospholipid complex (ERE-PC) was developed with an attempt to enhance the therapeutic effect by improving its dissolution profile and oral bioavailability. E. ribes, an ancient herb possesses anticancer, anti-fertility, antimicrobial and anti-diabetic activities. However, poor aqueous solubility of phytoconstituents of E. ribes, lowers the therapeutic effectiveness of the extract. Objective To prepare phytosomes of Embelia ribes fruit extract (ERE-PC) to improve in-vitro release profile and oral bioavailability. Method: ERE-PC were formulated by solvent evaporation technique using methanol as reaction mixture which aimed for its improved pharmacological activity. The complex formation was confirmed by FTIR, PXRD, DSC and microscopical studies. The prepared complex was evaluated for functional properties such as solubility, dissolution profile and in-vitro cytotoxic activity. Result: The data obtained from FTIR, DSC and microscopic studies confirmed the formation of phytosomal complex. Aqueous solubility of the extract significantly increased from 23.04 ± 0.0023μg/ml to 84.02 ± 0.0036μg/ml in ERE-PC. Particle size and zeta potential of optimized phytosome was found to be...
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Posted by admin on Jan 31, 2024 in |
Objective: To study the anti-arthritic effect of a Unani regimen comprising of Munḍij wa Mushil therapy along with Habb-e-Gul-e-Aak and to propose a safe and alternative therapy for RA. Materials and Methods: In this clinical trial, 30 diagnosed patients of RA fulfilling the inclusion and exclusion criteria were recruited and randomly distributed into two groups A&B with 20 patients in the test and 10 in the control groups, respectively. In Group A, Munḍij and Mushil therapy was given for 21 days once in the morning and then HGA for 28 days twice a day. Patients in Group B were treated with Hydroxychloroquine (HCQ) 200 mg twice a day for 49 days. All the patients were examined clinically before the treatment and follow-up was programmed 7th day, 21st day, 28th day, 42nd day, and 49th day. The efficacy was determined on subjective parameters as well as Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) and Visual Analog Scale (VAS). The biochemical and hematological investigations were conducted on baseline, 21st and,...
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Posted by admin on Jan 31, 2024 in |
Flurbiprofen (FP) is a phenyl alkanoic acid derivative and a family of non–steroidal anti-inflammatory drugs used in the treatment of arthritis. Flurbiprofen was formulated as a liposome using soya lecithin and cholesterol by thin film hydration method. It is then formulated as a stealth liposome to increase efficacy and reduce toxicity. Two polymers are used to prepare flurbiprofen as stealth liposomes i.e., PEG and PVP, and their effects are compared. The formulated liposomes and stealth liposomes were evaluated for various parameters like surface morphology, zeta potential, polydispersity index, drug content, percentage drug encapsulation, and in-vitro drug release. The optimized formulation (F2) containing a minimum concentration of cholesterol showed moderate stability and good entrapment efficiency. When compared to conventional liposomes, stealth liposomes showed more zeta potential values due to the effect of PEG 4000 and PVP K30.The zeta potential value demonstrated that stealth liposomes had enough charge to prevent liposome aggregation due to electric repulsion. The kinetic data analysis of formulations indicated that it fits the Higuchi model and follows...
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Posted by admin on Jan 31, 2024 in |
Background: Methicillin resistant Staphylococcus aureus (MRSA) is known to cause various infections ranging from minor skin and soft tissue infections to devastating septicaemia and endocarditis. The only drug that is currently most effective against MRSA infections is a glycopeptide, vancomycin. Due to continued exposure and injudicious use of this novel drug, cases of reduced susceptibility to vancomycin are appearing which could be complete resistance (VRSA), intermediate resistance (VISA) or heterogeneous resistance (hVISA). Aims & Objectives: To study: The antimicrobial susceptibility pattern of MRSA to commonly used antibiotics and vancomycin, teicoplanin and linezolid. The proportion of VISA and hVISA among MRSA isolates. Material & Methods: The study was conducted in Microbiology department of GGSMCH, Faridkot in which 150 MRSA isolates were processed as per standard microbiological protocols. Vancomycin susceptibility was studied by 3 methods: vancomycin screen agar using 6 mg/mL of vancomycin, micro broth-dilution and Epsilometer test using Hi-media Vancomycin-Teicoplanin graded E-strip. Results: Of 11726 specimens received, Staphylococcus aureus (S. aureus) was isolated from 230 specimens. 85.22% (196) were methicillin...
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Posted by admin on Jan 31, 2024 in |
Medicinal plants essential oils in aromatherapy, complementary medicine or in folk medicine, is known for a long time or is gaining importance as a significant alternative method for the treatment of various ailments. In the last few years a lot of scientific studies have been conducted to investigate the effects and mechanisms of action of these natural compounds and their influence on the central nervous system (CNS). In the present study essential oil obtained from the Skimmia anquetilia leaves (Rutaceae) was subjected to chemical analysis and the oil was investigated for its anxiolytic and antidepressant effects on rats. Chemical analysis of the leaf oil (1.2%) by gas chromatography and mass spectrometry (GC/MS) whereas, anxiolytic and antidepressant properties were evaluated by employing the social interaction test and hole board in rats. GC/MS studies of S. anquetilia oil indicated the presence of important anxiolytic phytoconstituents like linalool (10.085%), geranyl acetate (5.588%), geraniol (1.463%) and limonene (1.362%). The anxiolytic studies of SAO were conducted at doses of 25 and 50 mg/kg, body...
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