Posted by admin on Jun 30, 2017 in |
The leaves of Corchorus trilocularis possesses several bioactivities and is used in traditional medicinal systems. However, its anti-diabetic activity has not been scientifically investigated so far. The present study was carried out to evaluate the anti-diabetic activity of chloroform extract extract of Corchorus trilocularis (family: Tiliaceae) leaves in Streptozotocin induced diabetic rats. Chloroform extract showed presence of saponins, phytosterols, flavonoids, phenols, steroids and terpenoids. From the toxicity study it was observed that chloroform extract of Corchorus trilocularis (CECT) was nontoxic up to the dose of 2000 mg/kg body weight. In this study, animals received continuous oral administration of CECT for a period of 21 days at the doses of 200 mg/kg and 400 mg/kg body weight. The effect of CECT was compared with oral dose of 5mg/kg Glibenclamide. The results showed that the CECT significantly lowered the blood sugar level of hyperglycemic rats in a dose dependent manner. CECT reduced glycosylated hemoglobin, lactate dehydrogenase and creatinine kinase levels in streptozotocin treated animals. The extract also ameliorated oxidative stress Parameters...
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Posted by admin on Jun 30, 2017 in |
Background: Nagabhasma is a lead-based Ayurvedic medicine. Numerous reports indicate the toxic effects of lead used in this preparations on various systems of our body. But Ayurveda claims that the toxic effect of lead gets nullified thereby acquiring the medicinal property by following stringent and traditional method of preparation. Objectives: Therefore, the present study is designed to evaluate the effect of such detoxified lead in various stages of authentically prepared Nagabhasma on the histopathology of kidney in comparison with lead acetate treated and commercially available Nagabhasma treated animals. Materials and method: Less than the human equivalent doses of Nagabhasma at four different stages of its preparation were fed orally for 30 days and 60 days (short term and long term) to Wistar rats. The animals were even kept under observation for a period of two months after the treatment period and then the residual effect was studied. After the treatment and observation period, the animals were sacrificed to collect the kidneys for histopathological examination. Results: The histopathological results of...
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Posted by admin on Jun 30, 2017 in |
Mycobacterium tuberculosis (Mtb) is a pathogenic bacteria species in the genus Mycobacterium and the causative agent of most cases of tuberculosis. Tuberculosis (TB) is the leading cause of death in the world from a bacterial infectious disease. This antibiotic resistance strain lead to development of the new antibiotics or drug molecules which can kill or suppress the growth of Mycobacterium tuberculosis. The aim of this study is discovery a new drug molecules against the product of accD3 gene which play a vital role in mycolic acid biosynthesis pathway (MAP). As a first step accD3 gene amplified from the complete genome of Mycobacterium tuberculosis Iraqi isolates and sequenced, eight out of twenty of the resulted sequences were chosen to achieve the in silico studies, these sequences have deposited in GenBank database with strain names (ALQM1, ALQM2, ALQM3, ALQM4, ALQM5, ALQM6, ALQM7, ALQM8 ) and Accession numbers (LC006979, LC008196, LC009412, LC009414, LC034168, LC038020, LC041163, LC041368) respectively, two drug molecules resulted from in silico studies and used in the practical application to ...
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Posted by admin on Jun 30, 2017 in |
Background and aim: Thymoquinone (TQ), which is the most bioactive component of Nigella sativa (Black cumin), exhibits anticancer characteristics based on cell culture and experimental animal studies. However, molecular action mechanisms of these effects are not clear. MicroRNAs (miRNAs), small non-coding RNAs of approximately 22 nucleotides, are an emerging class of gene expression modulators with relevant roles in several biological processes, including cell differentiation, development, apoptosis, and regulation of the cell cycle. The purpose of this study was to investigate the potential impact of thymoquinone (TQ) on MCF-7 human breast cancer cell miRNAs. Materials and methods: The expression levels of miRNAs in MCF-7 cell and TQ treated MCF-7 cells were estimated by miRNA sequencing. The expressions of miRNAs were determined real-time qPCR. Results: We detected 10 down-regulated mirRNAs (hsa-miR-1, let 7c-5p, hsa-miR-15b-5p, hsa-mir-202-3p, hsa-miR-214-3p, hsa-miR-210-3p, hsa-miR-31-5p, hsa-miR-424-5p, hsa-miR-497-5p, hsa-miR-98-5p) and 2 up regulated miRNAs (hsa-miR-22-3p, hsa-miR-132-3p) in TQ treated groups, comparing with control group. These findings highlight the effects of TQ miRNA profile and molecular mechanism on MCF-7 cells....
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Posted by admin on Jun 30, 2017 in |
The objective of the current study was to differentiate contraceptives for their effects on carbohydrate metabolism. This is a cross sectional design that was conducted in different clinical settings of urban population who belong to low socio economic status by convenience sampling method. Females representing fertile age ranging from 26 to 32 and maintained on different types of hormonal and non hormonal contraceptives (OC; Oral contraceptives, DMPA; Depo-medroxy progesterone, NET-EN; Norethisterone , Implant and non hormonal intrauterine contraceptive device (IUCD) are enrolled and invited in 12 hrs fasting to give their blood samples. Fasting blood sugar is analyzed by GOD–PAP method. All the readings were fed on MS- Excel to evaluate mean ± S.D, Comparative effects were represented by percentile differences between different groups. The present study concluded that all hormonal and non hormonal methods posed no deleterious effects on carbohydrate metabolism. These minimized risks may be due to young age and good monitoring of therapy in clinical practices and could be threatening in the areas where these are...
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