Posted by admin on Apr 27, 2017 in |
Today’s generic drugs manufacturing industry need robust analytical methods for analysis of generic drug products to provide best quality pharmaceutical formulation. Entacapone, Levodopa and Carbidopa tablets is such a product which is used for the treatment of Parkinson’s disease. This paper presents a robust HPLC method that has been developed for estimation of % drug release of Entacapone in this multi component drug formulation. HPLC column used for separation was Cosmosil 5PE-MS 150mm x 4.6 mm with 5 micron particle size. Combination of phosphate buffer pH 2.5, acetonitrile and methanol was used for mobile phase in isocratic mode with UV detection at 280 nm. The method was validated to ensure suitability of the method for quantitative determination of % drug release of Entacapone in presence of Levodopa and Carbidopa in this triple drug combination pharmaceutical formulation. Proposed method was found to be specific, precise, robust, accurate and linear in range 0.066 to 0.33 mg/mL of...
Read More
Posted by admin on Apr 12, 2017 in |
Staphylococcus aureus biofilm has been known as one inducing factor for the bacteria’s resistance to various antibiotics. One strategy which may increase the efficacy of the antibiotic is by combining the antibiotic therapy with antiinfective from natural resources. This research was evaluating the potency of three known antiinfective essential oil derived from leaves of Piper betle L., Ocimum basilicum L. forma citratum Back and Cymbopogon citratus L., in a combination with antibiotics i.e. chloramphenicol, streptomycin, and erythromicin towards S. aureus ATCC 29213. The essential oils were obtained by steam-hydrodistillation of the fresh raw materials. Microdillution technique combined with colorimetric was used to determine the biofilm inhibition. Crystal violet was used for biofilm staining of which the reading was performed on a microplate reader. Fractional Inhibitory Concentration Index (FICI) values was evaluated based on the comparison of % inhibitory obtained from the essential oils and the antibiotics in a single and in a combination. The essential oils alone has the PMIC50 (planktonic) values as follows, 0.2% (P. betel), 0,3% (C....
Read More
Posted by admin on Mar 31, 2017 in |
Cjanus cajan (L.) Millsp belonging to the family Fabaceae commonly known as pigeonpea or arhar. The present study conducted to assess the phytochemical and pharmacognostic in terms of organoleptic, fluorescence analysis and physicochemical parameters of seed coat and cotyledon of C. cajan. Differential extraction yielded of seed coat extracts of petether, chloroform, ethanol and aqueous shows 6.3%, 1.14%, 11.36% and 9.32% and cotyledon extracts of pet ether, chloroform, ethanol and aqueous shows 7.34 %, 4.25%, 10.45 % and 8.45 % respectively. Physicochemical parameters such as total ash, acid insoluble ash and water soluble ash of seed coat and cotyledon were found to be 14.5% and 11.5%, 8.35% and 7.36%, 2.11% and 3.39% respectively. Further the mineral elements like zinc, magnesium and copper content were found more in seed coat whereas, maximum content of iron were observed in cotyledon. The fluorescent analyses of powdered drug play an important role in the determination of quality and purity of the drug. Phytochemical analysis revealed the presence of glycosides, tannins, flavonoids, phenols, lignins,...
Read More
Posted by admin on Mar 31, 2017 in |
Different new compounds derived from 2-(3-flourobiphenyl-4-yl) propanohydrazide [2] as starting material were synthesized. Reaction [2] with maleic anhydride, succinic anhydride and phthalic anhydride gave the corresponding cyclicimides [3a-c]. The hydrazone derivatives [4a-d] were obtained by condensation of compound [2] with 2,4-dimethoxybenzaldehyde, 4-(N,N-dimethylamino)benzaldehyde, 3-hydroxybenzaldehyde and 2-hydroxybenzaldehyde. The cyclization of [2] with CS2 in presence potassium hydroxide and hydrazine hydrate gave 1,2,4-triazole compound [5], that condensation either with 2,4-dimethoxybenzaldehyde, 4-(N,N-dimethyl amino) benzaldehyde, 3-hydroxybenzaldehyde and 2-hydroxybenzaldehyde to give the corresponding Schiff-bases [6a-d]. The cyclization of hydrazone derivatives [4a-d] with phenyl isocyanate, maleic anhydride, succinic anhydride and phthalic anhydride gave the corresponding aza-β-lactams [7a-d], oxazepine [8,9,10 a-d]. The structures of new compounds are supported by FT-IR, 1H-NMR and 13C-NMR. Compounds [3c], [4c], [8d], [7d] and [4b] were evaluated in vivo for their anti-inflammatory activity comparable to the standard drug flurbiprofen. Compound 2-(2-hydroxyphenyl)-3-[2-(3-fluorobiphenyl-4-yl)] propan- amido-2,3-dihydro-1,3-oxazepine-4,7-dione [8d] showed anti-inflammatory activity more than the standard drug flurbiprofen, in the egg-albumin induced paw edema in...
Read More
Posted by admin on Mar 31, 2017 in |
Ayurveda is one of the oldest systems of medicine that uses plants and their extracts for treatment and management of various diseased states. Nyctanthes arbour – tristis Linn. (Family Oleaceae), which has long been used traditionally for various medicinal purposes in the Indian sub continent. Each part of the plant has some important medicinal value and is thus commercially exploitable. It is now considered as a valuable source of several unique products for the medicines against various diseases and also for the development of some industrial products. The present study is to focus on the potential phyto-chemicals and pharmacological activity of plant N. arbor – tristis. In view of this, the study was designed to investigate for chemical constituents, anti-cancer and anti-inflammatory activities of solvent leaf extracts. Phytochemical screening revealed the presence of alkaloid, steroids etc. The extract exhibited significant anti-cancer and anti-inflammatory properties and the observed biological activities in this study provide scientific validation of ethnomedicinal use of this...
Read More
