Posted by admin on Aug 31, 2017 in |
In recent decades, advances in nanoscience have resulted the rapid development of many novel technological applications for nanoparticles (NPs) in various in Biomedical Sciences. Iron is essential for eukaryotic biochemistry and is an important element in several metabolic processes such as, carrier for oxygen transportation, cell respiration, synthesis of genetic material and protein synthesis. The nematodes Caenorhabditis elegans (C.elegans) represent an excellent organism to investigate the phenotypic effect of different type of nanomaterials on the molecular, cells and entire organism level. C.elegans is widely used in studies of homeostasis, neurolagenerative and metal toxicity. The size of iron oxide and other nanoparticles plays a major role in target cell uptake and elimination from the body. The excess iron damage nucleotides (DNA/RNA) and Protein due to catalyzes the generation of Reactive Oxygen Species (ROS) , while cellular iron deficiency causes cell cycle arrest and cellular death of an organism. Systematic storage and trafficking is required to regulate supply of iron while preventing it from catalyzing unwanted reactions. We have used 36...
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Posted by admin on Aug 31, 2017 in |
The present study elucidates the secondary metabolite profile of leaves of Pseudarthria viscida, one of the main ingredient of dasamula in Ayurveda. Alkaloids, phenolic compounds and flavonoids were estimated in different solvent-extracts. Acetone extract of leaves showed highest content of phenols and flavonoids. The leaves are quite rich in alkaloids and is found to be 1.9 mg g-1. GC-MS analyses revealed the presence of 20 major volatile organic compounds and were identified by their mass fragmentation pattern in comparison with NIST library. Most of the compounds have antioxidant, anti-inflammatory, antimicrobial and anticancer properties. The present study has identified a major compound Neophytadiene which is a well-known enzyme...
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Posted by admin on Aug 31, 2017 in |
Bacterial pathogens are known to pester Mankind from times immemorial; even though significant efforts in getting rid of these harmful microbes have been made the results are very faint with only few organisms that have been eradicated, such as Small pox virus. But the efforts to eradicate bacterial diseases have shown no passable results as in case of viruses. This is due to the exceptional adaptation and transformation abilities of bacteria to varying environmental Conditions. Though a large number of antibiotics are being used from decades now, there are no affirmative solutions available due to resistance developed towards antibiotics by these bacteria. This resistance developed by the bacteria calls for dire necessity to discover new drugs which can at least reduce the hazards posed by these microbes if not eradication. Hence in this study we have focused on bio prospecting of Clerodendrum paniculatum and Saraca asoka against the highly virulent and extremely adaptable organisms E. coli and K. pneumoniae which currently pose a severe threat to humans due to...
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Posted by admin on Aug 31, 2017 in |
A series of novel 2-Benzamido-3-(4-nitrophenyl)-N’-(substituted) benzylidene-acrylohydrazide (4a-l) analogues were designed by incorporating the few pharmacophoric fragments to enhance the activity profile of molecules. Title compounds were synthesized by the microwave irradiation of α-Benzamido-(4-nitro)-cinnamahydrazide (3) reveals with aromatic/ heteroaromatic aldehydes under acidic conditions, characterized by IR, 1H & 13C NMR, Mass and evaluated for antimicrobial activity by using Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and Candida albicans strains. Compounds 4e and 4k exhibited significant protection against bacterial strains (MIC: 3.12-50µg/ml; 3.12-100µg/ml respectively) and compound 4h showed better activity (MIC: 12.5 µg/ml) against fungal strain. And also molecular docking interactions with FAB protein for evaluating the antimicrobial activity was done by using XP GLIDE module of Schrodinger suite and this study highlights, all the analogues exhibited significant affinity towards the 5BNM (FAB Protein) have good docking scores (-4.51 to -8.78) than standard drug ciprofloxacin (-4.74). Of the 12 new analogues, compound 4e was identified as most active ligand with good activity profile against bacterial strains and also have higher...
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Posted by admin on Aug 31, 2017 in |
Aim and Objective: To evaluate the antioxidant property of fish oil by its free radical scavenging activity using three different antioxidant models. Materials and Methods: In vitro antioxidant activity of fish oil was evaluated by DPPH radical scavenging activity, lipid peroxidation, ABTS radical scavenging activity using olive oil as positive control. Different concentrations of the fish oil, 0.02 – 4 mg/ml were used for the free radical scavenging activity by different method and the IC50 value was calculated. Results: Different concentrations of the fish liver oil exerted a dose dependant free radical scavenging activity on the different antioxidant models used in the study. The maximum inhibition was 96.87±0.76% at 4mg/ml in case of DPPH free radical scavenging activity with IC50 value 0.148mg/ml. The maximum inhibition on LPO activity was 96.10±0.38% at 4mg/ml with IC50 value of 0.265mg/ml. The fish oil also exerted its maximum inhibition 90.21 2.10% on ABTS free radical scavenging activity at 4mg/ml with IC50 value of 0.331...
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