Posted by admin on Jun 30, 2017 in |
Acid amine coupling of 1H-indol-6-yl(piperazin-1-yl) methanone with different acids has been synthesized in presence of HATU reagent, DIPEA base with DMF solvent at 45 °C 30 mins to yield Indole piperzine derivatives 3a-j. The structures of all the synthesized compounds have been characterised by elemental analysis and spectral studies like 1H-NMR and LCMS. Some selected Indole piperazine derivatives have been tested for antimicrobial activity using by Disc diffusion method and in vitro antioxidant activity using DPPH Assay method. Three compounds 3a, 3f and 3h have shown significant antimicrobial and one compound 3b have shown in vitro antioxidant...
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Posted by admin on Jun 30, 2017 in |
The present investigation revealed the phytochemical constituent, antioxidant potential and correlation between antioxidant activities with total phenol and flavonoid contents of fruit extracts of Pyrus pyrifolia and Prunus domestica from local Himalayan region of Himachal Pradesh, India. The total phenolic content was estimated by using Folin-Ciocalteu reagent method, whereas, flavonoid content was quantified using aluminium chloride method. The antioxidant activity was evaluated by using DPPH, FRAP and Nitric oxide (NO) scavenging assay. The total phenolic content was found to be 159.86±2.91 and 145.56±2.43 µg/ml gallic acid equivalents. Similarly, flavonoid content was 48.69±1.90 and 284.27±2.16 µg/ml rutin equivalents for P. pyrifolia and P. domestica, respectively. The antioxidant activity values of DPPH scavenging for fruit extract were found to be (IC50-17.37 and 9.47 µl/ml), FRAP activity values were (IC50– 15.51 and 6.13 µM Fe (II) equivalents) and Nitric oxide scavenging values were (IC50– 21.49 and 30.11 µl/ml) for P. pyrifolia and P. domestica respectively. The investigation revealed significant correlation between total phenolic and flavonoid contents with antioxidant activities of fruit extracts....
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Posted by admin on Jun 30, 2017 in |
Ascidians are rich source of novel bioactive agents as potential source of therapeutic drugs. Evaluation of antioxidant potential of ascidians, Eudistoma viride and Didemnum psammathodes extracts was undertaken. In DPPH radical scavenging assay E. viride II showed 34% to 93% scavenging activity and D. psammathodes II showed 45% to 96% scavenging activity in different ascidian concentrations. In reducing power assay the ascidian fraction E. viride II and D. psammathodes II showed 2.3 and 2.23 reducing power in 100µgmL-1. In Hydroxyl (OH) radicals scavenging activities the ascidian E. viride II and D. psammathodes II showed high radical scavenging activity 96% and 98% at 100 µgmL-1 concentration. In H2O2 scavenging assay the ascidians fractions E. viride II and D. psammathodes II showed 78% and 89% of at 100µgmL-1 concentration. In nitric oxide radical scavenging activity, the ascidians fractions E. viride II showed 64% at 100µgmL-1 and D. psammathodes II showed 72% at 100µgmL-1 concentration. The finding throws light on the higher efficiency of scavenging activity of natural products improved to synthetic...
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Posted by admin on Jun 30, 2017 in |
Metronidazole is a class I drug in biopharmaceutics classification system (BCS) and widely used in Pakistan, various available brands in local markets along with innovator brand to serve a large population. In-vitro dissolution testing can be used as waiver for in-vivo bioequivalence studies of generic brand interchangeability for Class I drugs i.e., highly soluble and highly permeable. The objective of the study was to determine immediate release tablet dosage form of metronidazole to fulfil the requirements of 85% drug release in pH 1.2, 4.5 and 6.8 recommended by WHO. In this study physicochemical quality attributes, price comparison and dissolution studies were performed on three available brands of metronidazole 400 mg, one of them is innovator brand and the two others are test brands. The dissolution profiles were compared by using similarity factor (f2) that were analyzed by UV spectrophotometer at 278 nm wavelength. The test brands were very cheap in cost as compared to innovator brand but both the test brand were failed the dissolution test in all mediums...
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Posted by admin on Jun 30, 2017 in |
An approach to determine the relationship between independent process variables and their two level four factor partial factorial design was adopted, composite of experiment design was applied to optimize a tablet formulation of Tetrabenazine (TBZ) Tablets 25mg containing high percentage of Lactose Anhydrous, Sodium starch Glycolate, Magnesium Stearate and Starch / Lactose Ratio. The particle size distribution of Lactose Anhydrous is used as dependent variable and Sodium starch Glycolate, Magnesium Stearate and Starch / Lactose Ratio were used as independent variables for optimizing some tablets response parameters. Response parameters for final TBZ Tablets were percentage of TBZ dissolve at thirty minutes. Design of Experiments (DOE) is an organized effect on the response variable. 24 partial factorial designs were applied in this research work. The models were validated for accurate prediction of response characteristics and use to identify the optimum formulation. The results that an optimum TBZ 25mg tablets having a volume similar to commercial products can be produced by dry granulation process utilizing Lactose...
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