Posted by admin on May 31, 2017 in |
The aim of the present studies is to evaluate the cytotoxic effects of newly formulated polymeric nanoparticles (ETP NP’s) of Escitalopram oxalate (ETP) on NB41A3 cell lines. They were made by ionic gelation method with particle size range between 60 – 115nm, showing spherical morphology. Moreover, zeta potential of the same was recorded as -1.89 ± 0.052 mV and its permeability release showed 98.45 ± 1.82 % in 24 hour. The cytotoxicity evaluation of the optimized formulation (A3) included qualitative method of cell morphology (microscopic and DAPI staining evaluation) and quantitative colorimetric assays like – neutral red (NRU), MTT and LDH. The results suggested that, in presence of ETP, there was a remarkable decrease in cell count whereas; ETP NP’s showed lesser cytotoxicity. performed. Further, in NRU membrane integrity was assessed and it showed 95.2 ± 0.83 % and 72.2 ± 0.56% of cell viability in ETP NP’s and ETP pure respectively. However, MTT assay summed up the cell viability up to 93.6 ± 0.68 % in ETP NP’s...
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Posted by admin on May 31, 2017 in |
The excessive exploitation of medicinal plants during recent years raises the issues regarding their quality, safety and efficacy. In this respect, there has been a rapid increase in the standardization of medicinal plants with therapeutic potential. In spite of the new techniques, identification and evaluation of plant drugs pharmacognostic study is more cheap and reliable. Abrus precatorius L. of family Fabaceae is native to India and is locally known as Rati. It is widely employed in traditional medicinal systems. The present investigation deals with the macroscopic and microscopic evaluation of the seeds of this species to record the parameters of diagnostic value. Macroscopic studies comprised of shape, size, taste, colour, odour etc. Microscopic studies were done by using trinocular microscope. Anatomy of the seed was studied by taking transverse sections. Such information will be used for further therapeutical and pharmacological evaluation of the species and will also help in maintaining the quality and purity of sample. Moreover, the study would also serve as a valuable tool in drug identification...
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Posted by admin on May 31, 2017 in |
Solubilization is depends on solute-solvent interaction, dissociation of solute in solvent into ionic form, temperature, pressure, hydrogen bonding, dielectric constant, polarity and non-polarity of substance, pH, etc. Partition coefficient was determined in two immiscible solvents in aqueous and organic layer. Benzoic acid was selected for present study for its solubilization in different pH buffer solutions (acidic, neutral and basic) and for partition coefficient study at room temperature. Solubility of benzoic acid in different pH buffer solutions was analyzed by titration method. Most common two immiscible solvent such as water as hydrophilic solvent and benzene as hydrophobic solvent were selected for partition coefficient study. The drug benzoic acid was partitioned between these solvent by shake-flask method and analyzed the concentration of drug in both solvents by acid-base titration method. Solubility and distribution of benzoic acid between benzene-buffer solution of pH 4.0, 7.0, 9.0 and distilled water have been determined. It was observed that the benzoic acid remains un-dissociated monomer molecular form in aqueous layer and as dimer associated form in...
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Posted by admin on May 31, 2017 in |
Borassus flabeliffer Linn. (Palmyra Palm) is widely distributed in the Northern parts of Sri Lanka. Its leaves, fruits and pith have various uses. Palmyra based industries provide living for many families in that region. Palmyra pulp obtained from the ripe fruit is used in many traditional food items in the Jaffna Peninsula and surrounding areas. It is also being used in folk medicine to cure various diseases. In this study, total phenolic content and antioxidant activity of aqueous palmyra fruit pulp extracted from fruits of Kilinochchi, Anamaduwa and Batticalo districts of Sri Lanka were evaluated. The total phenolic content of the pulp extracts of fruits obtained from Anamaduwa district showed the highest value (9.297 ±0.018 mg GAE/mg extract). DPPH Radical scavenging activity was seen highest in the Anamaduwa samples (3.065 ±0.176 mg ml-1) and lowest in Batticalo sample (7.466 ±0.156 mg ml-1). The total antioxidant capacity based on the phosphomolybdenum assay also indicated the highest value in the Anamaduwa sample (68.171± 2.068 mg AE/g extract) and lower value in...
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Posted by admin on May 31, 2017 in |
A reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for the determination of Atenolol (ATN) in bulk and pharmaceutical formulation. The method was optimized selecting chromatographic conditions of 60: 40 acetonitrile: water, Inertsil_ column(ODS-3 250 mm × 4.6 mm 5 ?m), 20 ?L injection volume, flow rate of 1 mL/min at ambient temperature (30 °C), and 276 nm. Using two HPLC systems of first HPLC system coupled with PDA detector and the second HPLC system coupled with UV detector showed no big difference in the method results. The method was validated giving good precision (RSD% < 1), acceptable linearity (R2 > 0.998), and low LOD and LOQ (0.5 and 1.5 ?g/mL, respectively) on both systems. Successful application on pharmaceutical dosage tablet form gave high recovery of 97.7%. The proposed method is economic, simple, and rapid and hence can be employed for routine analysis in quality control...
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