Posted by admin on Dec 31, 2023 in |
Background: Sodium fluoride (NaF) is an inorganic fluoride salt used for the treatment of dental caries topically. It is also used in water fluoridation to prevent tooth decay. It has been reported to be associated with a lot of harmful effects, including neurotoxic effects. The aim of our study was to evaluate the chronic effects of NaF on the models of cognition in adult mice. Methods: Forty mice were randomly divided into four equal groups. NaF was administered dissolved in drinking water. The control group was Group I and received distilled water. Oral NaF at doses of 10mg/L, 20mg/L and 30mg/L respectively was given to Group II, Group III, Group IV. The influence of fluoride exposure on cognition was assessed using forced alternation test and spontaneous alternation test using Y-Maze. Evaluation was done at baseline, at the end of one month, two months and three months of NaF administration. Statistical analysis was done with the help of the Wilcoxon Signed Rank Test (within the group), Kruskal Wallis Test (between-group)...
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Posted by admin on Dec 31, 2023 in |
The bio-active compounds present in Terminalia arjuna (Roxb. ExDC) have anti-oxidant, anti-proliferative, anti-obesity, anti-hyperglycemic, and lipid-lowering activity. In addition, it has cardioprotective, hepatoprotective, and renoprotective properties. The study evaluated in-vivo toxicity profile of the methanolic extract of Terminalia arjuna (META) bark on male albino Wistar rats before undertaking further investigation on the use of this extract as a potential therapeutic agent against such conditions. Animals were administered with 300 and 2000 mg/Kg b.w./day of META bark for 14 days; and 250, 500, and 1000 mg/Kg b.w./day of META bark for 28 days for acute and sub-acute toxicity respectively. The outcome of the acute toxicity study revealed no signs of mortality or general behavioral alteration in treatment groups in comparison to the control group. The sub-acute toxicity study revealed, there is no significant influence (p<0.05) of META bark in food consumption and percentage weight gain in the animals. No significant changes (p<0.05) in hematological parameters as well as in biochemical parameters were observed. Histopathological examination showed normal cellular architecture of...
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Posted by admin on Dec 31, 2023 in |
A quick and affordable analytical method validation for the determination of Ascomycin content in Tacrolimus API by HPLC UV-detector was developed with respect to the accuracy, precision, linearity, selectivity, robustness, limit of quantification, limit of detection, according to ICH guidelines. The extraction of samples was performed using HPLC –LC Solutions Chemstation system manufactured by WATERS by using X-TERRA, C18 (4.6 x 150mm, 3.0μm) in the Presence of mobile phases A&B composed of 6 ml orthophosphoric acid and Acetonitrile: Tert-butyl Methyl Ether (81:19) in the ratio of 4: 1 and 1: 4. The estimated samples were then analysed using an UV visible code 2487 manufactured by Waters. With a linear calibration curve spanning from the mean recovery of method precision and system precision 140765 and 142973 and %of relative standard density is found to be 0.30% and 0.87%. With retention time of Ascomycin 29.5mins and Tacrolimus 33.4 min. Column oven temperature of 60 0C at a flow rate of 1.5mL/min and wavelength is found at 220 nm the total run...
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Posted by admin on Dec 31, 2023 in |
Objective: The present study was designed for the synthesis, characterization, biological evaluation, and MTT assay of some novel thiadiazole derivatives as anti-tubercular agents targeting decaprenylphosphoryl beta-d-ribose2’ epimerase-1(DPRE1). Methods: The molecular docking study for titled compounds was performed from Autodock 4.2. Software http://autodock.scripps.edu/, pdb file was generated by chem3D.pro software tool. The binding pose for the significant compounds was visualized by Biovia, the Discovery studio visualizer. The selected molecules were synthesized and recrystallized several times to reach the expected purity. All the purified compounds were characterized by various spectral analytical techniques and evaluated for anti-mycobacterial activity against tuberculosis H37RV strain by Microplate Alamar Blue Assay (MABA) method and cell line studies. Results: The experimental results showed that the Compounds SDK3 and SDK5 have an anti-tubercular activity in the Concentration of 3.12µg/mL. Cell line studies for proprietary compounds have been carried out by MTT assay using HEK (Human embryonic kidney cells) method. It also correlated with the highest docking score. Conclusion: The development of the SDK3 and SDK5 structures will produce...
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Posted by admin on Dec 31, 2023 in |
Triphala churna is a poly-herbal preparation that has anantiquity of usage in the conventional Indian medical system. It is rich in antioxidants and has excellent therapeutic efficacy. According to ayurveda, the churna is made using the raw ingredients Amalaki (Indian Gooseberry), Haritaki (Indian Hog Plum), and Bibhitaki (Vibheetaki) in an equal proportion (1:1:1). The present investigation purpose was to establish the Triphala’s antimicrobial effect on different types of bacterial stains and its antioxidant effects. Numerous cross checks examinations have been conducted, such as phytochemical detection, physico-chemical analysis, pharmaceutical analysis, to evaluate herbs’ quality attributes, including formulations’ assurance and effectiveness. A comparison of the antioxidant and antibacterial capabilities of polyherbal drug’s aqueous and ethanolic extract follows. Antioxidant activity of Triphala churna was established by FRAP assay and 2,2-diphenyl-1-picrylhydrazyl free radical scavenging methods. By using broth dilution, antibacterial potential was assessed. Triphala reported the presence of valuable bioactive components, including phenols, alkaloids, and flavonoids, which may be in charge of certain biochemical activities, was found, according to the results. Ascorbic acid-like...
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