Posted by admin on Mar 31, 2017 in |
Ginger has shown to benefit in muscular pain due to eccentric exercise and in osteoarthritis but a later review denied these properties due to uncertainties. To ascertain the reality, this study was proposed. Methods: (A) Albino rats (n=6-12) were administered G.J at single dose (4 ml/rat, p.o) as single administration (acute) and chronic treatment 4ml/rat p.o over period of 7 days. Following this assessment was done. Effect of treatment with G.J acutely and chronically (7 days) administered, was assessed. Parameter used during assessment was head twitches. Results: Chronic treatment of ginger-juice induced the head twitches significantly. Acute treatment with ginger-juice did not affect this activity. Chronic treatment increased the number of head twitches induced by lithium. Conclusion: Lithium induced head twitches were enhanced after chronic administration of...
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Posted by admin on Mar 31, 2017 in |
Now a day the exposure of multi-resistance human pathogens has challenged our existing defense to save human being. To minimize such alarming issue, identification of naturally-occurring antimicrobial agents from plant origin has become a realistic and powerful tool to the researchers. Therefore the aim of this study was to investigate the antimicrobial potential of the ethanolic extracts of different parts of Abelmoschus moschatus (A. moschatus) against some important human bacterial and fungal pathogens. Different parts (leaves, stem bark, fruits pulp and seeds) of A. moschatus were extracted with ethanol to get crude ethanolic leaves (LE), stem bark (SBE), fruits pulp (FPE) and seed extracts (SE). Antimicrobial activity of LE, SBE, FPE and SE were assessed by disk diffusion and serial dilution method. All the extracts showed moderate antimicrobial activity against all tested pathogens. Among the extracts LE was comparatively highly sensitive against all tested pathogens. In case of S. dyesenteriae and S. sonnei zone of inhibition of LE (26 and 28 mm diameter with MIC 31.25 and 62.5 µg/ml;...
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Posted by admin on Mar 31, 2017 in |
The early identification of infection from sterile inflammation is still challenging step in nuclear medicine. Therefore, several radiopharmaceutical agents have been examined to find the solution of this dilemma in clinical practice. Recently 99mTc-Ofloxacin has been developed as an infection-seeking radiotracer. This investigation was launched to examine the sensitivity and selectivity of new developed radiotracer to distinct septic from aseptic lesions. The freeze-dried new developed kits were reconstituted by the 740 MBq (20 mCi) freshly 99mTcO4. The radiochemical purity of 99mTc-Ofloxacin was undertaken by thin layer chromatography (TLC) and Radio high performance liquid chromatography (Radio-HPLC) analysis. Twenty adult, male NMRI rats were randomly divide into two groups equally. Infection was induced by S. aureus and sterile inflammation created by Carrageenan test. Then radioisotope studies have been performed after intravenous administration of radiotracer into the contra lateral tail vein in all studies. TLC and Radio-HPLC investigations indicated that the infection-seeking radiotracer could be prepared with appropriate yield. All lesions were visualized by scintigraphy imaging. The quality of images was good...
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Posted by admin on Mar 31, 2017 in |
Probiotics have been used to protect hosts from pathogens of gastrointestinal and reproductive systems, but their ability to protect against systemic pathogens is largely unexplored. In this study we ask whether orally administered bacteria and yeasts can protect Drosophila melanogaster against the opportunistic fungal pathogen Aspergillus flavus. Flies were fed an artificial diet mixed with live microorganisms for one day prior to infection with A. flavus, and mortality was recorded every day for 8 days. Seven microorganisms were tested; of these, Bacillus cereus (ATCC 13061), Candida inconspicua, Issatchenkia hanoiensis, and Klebsiella sp. significantly decreased mortality of flies subsequently infected with A. flavus compared to controls infected with A. flavus alone. Heat-killed microorganisms did not protect flies, suggesting that the probiotic effect observed was not caused by improved nutrition. D. melanogaster is a good model organism to study microbial interactions with hosts and test the effects of potential probiotics against...
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Posted by admin on Mar 31, 2017 in |
To develop pulsatile microspheres of amlodipine and nifedipine drug release, a blend of polymer and solvent were used with pulsatile principles of drug delivery system to modified drug release pattern on and to release the drug at a particular time on the basis of lag time. In this study, we assessed the in vitro drug release pattern of the formulation prepared from different polymers ratio with the drugs of calcium channel blockers i.e. Amlodipine and Nifedipine. Different formulations of Amlodipine and Nifedipine were prepared using polymers Eudragit RS100, Eudragit S100, Carbopol 971P and polyvinyl alcohol. Optimum formulations were selected from both the drugs of pulsatile microsphere formulation such as formulation N12 from Nifedipine drug and A5 from amlodipine drug by conducting evaluation parameters for microsphere. In vitro studies were carried out for both the formulation and compared to each others. Kinetics drug released kinetics model fitted for both the drugs of optimum formulation for N12 and A5. Formulation N12 showed first order kinetics model and formulation A5 showed zero...
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