Posted by admin on Dec 31, 2016 in |
Although technologies are emerging day to day and bioinformatics is also growing with number of databases developed, but being a very important factor in cancer invasion and metastasis, MMPs have no exclusive database. Matrix Metallo-Protienase (MMP) is a major class of proteolytic enzymes that plays a dominant role in ECM degradation. Every time researchers are searching and gathering information from different places and next researcher repeating the same. In this study we developed a database exclusively designed for the MMPs and their...
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Posted by admin on Dec 31, 2016 in |
This study aimed to evaluation of healing potential of Sambucus ebulus in full-thickness wound model in rats. A dorsal skin wound was created in rats. The 5% ointment applied to the wound area once a day for 12 days. Healing process was compared histologically between the groups. Parameters of wound healing were evaluated in the wound area. Extract improved wound contraction and closure. Histopathology study certified an organization of wound tissues. Extract reduced the number of inflammatory cells, increased wound-healing rate, epithelialization and significantly improved collagen formation. Granulation tissue formation was more organized when compared with control groups. S. ebulus showed positive effects on wound healing process and improved healing...
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Posted by admin on Nov 30, 2016 in |
Adenosine has been proposed as a key factor regulating the metabolic balance between energy supply and demand in skeletal muscle. Adenosine A2B receptors are expressed across multiple tissues including skeletal muscle. Previously, it has been identified the robust (in vitro) induction of the nuclear receptors (NR4A) subgroup following adenosine A2B receptors stimulation in rat skeletal muscle cells. This may be concomitant with changes in the expression of genes involved in the regulation of nutrient metabolism. Considering that skeletal muscle is a primary tissue responsible for glucose disposal, glucose oxidation and insulin resistance, this study investigated a potential functional role for adenosine A2B receptors in rat L6 skeletal muscle myotubes in terms of glycolysis (HKII and PFK), and insulin resistance/glucose oxidation (PDK4) using a transcriptional approach (QRT-PCR (Probe-based)). For the first time in rat skeletal muscle cells, the results of the present study have shown that stable adenosine analogue NECA alters the expression of a number of genes (HKII, and PFK). Moreover, this is the first study demonstrating that selective...
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Posted by admin on Nov 30, 2016 in |
Back ground: Use of proper analgesics as adjuvant to balanced anaesthesia can calm the patient, reduce stress and decrease the need of higher doses of inhalation agents. Some examples of analgesics are opioids, NSAIDS, NDMA receptor antagonists (ketamine). Nalbuphine is a synthetic opioid agonist-antagonist analgesic of the phenantherene series. We conducted this study with the aim to observe the effect of nalbuphine as an adjuvant to balanced anaesthesia on intra operative hemodynamics and analgesic requirement in perioperative period. Material and method: prospective, observational study was carried out in 70 adult patient of ASA grade II -III, aged 18 to 60 years, undergoing otolaryngorhinological surgeries. After informed consent patients were given routine premedication given with inj. Nalbuphine 0.1 mg/kg i.v. General anaesthesia was given and subsequent 1/5th doses of the drug administered at every 30 minutes intra operatively. Intra operative hemodynamic monitoring was done. In post-operative period inj. Diclofenac 1.5 mg/kg i.m as a rescue analgesic was given when VAS score was ≥ 4. Observation results: Maximum rise in pulse...
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Posted by admin on Nov 30, 2016 in |
Background: Polyherbal formulation is the trend of modern pharmacognosy and holistic approach in the treatment of uncurable diseases such as cancer. Medicinal plants contain many bioactive compounds that give different pharmacological activities. Therefore, it is important to investigate the quality of new polyherbal formulation by identification of bioactive markers. Objective: To identify apigenin (1) , luteolin (2), b-sitosterol (9), 3’-hydroxy-5, 6, 7, 4’- tetra methoxy flavone (8), quercetin (3), vanillin(7), gallic acid (5), benzoic acid (6) and rutin (4) as biomarker from different fractions of the new polyherbal formulation of Clinacanthus nutans and Elephantopus scaber by RP-HPLC gradient method. Methods: A Shimadzu HPLC was utilized to perform the analysis which was equipped with an autosampler, column oven, and UV/VIS detector. An HPLC column used was Merck Licrochart Purospher Start RP 18 column (250mm, 4.6 mm i.d, 5μm pore size). The temperature was maintained at 40.0 ºC throughout the study. The mobile phase isocratic method with acetonitrile 100%. The flow rate was 0.5 mL min−1. Absorbance was observed at λ...
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