Posted by admin on Jan 31, 2024 in |
Ramipril is an angiotensin-converting enzyme inhibitor and is used in treating high blood pressure. Hydrochlorothiazide is a diuretic drug that increases urine output. High-Performance Thin-Layer Chromatography is a sophisticated form of thin-layer chromatography which is a separation technique and is used in various bioavailability studies to study marketed formulations as well as novel therapeutic agents. We aimed to develop and validate a simple, precise, accurate, and rapid HPTLC method for the simultaneous estimation of Ramipril and Hydrochlorothiazide in the combined solid dosage form. Precoated silica gel G60F254 was used as the stationary phase. The mobile phase used was a mixture of methanol: toluene: ethyl acetate: glacial acetic acid (1:6:3:0.5 %v/v). Spot detection was carried out at 210 nm. Validation of the method was done in terms of linearity, accuracy, precision, and specificity. A linear calibration curve was obtained between 2000 to 12000 ng/spot for Ramipril and 500 to 3000 ng/spot for Hydrochlorothiazide. The limit of detection and the limit of quantification for the Ramipril was found to be 434.1...
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Posted by admin on Jan 31, 2024 in |
Aim and Objective: The present study was done to assess the prevalence of polypharmacy and to study its consequences in elderly people. Methodology: The study was prospective observational study, done in 500 elderly patients attending the Department of General Medicine in rural tertiary care teaching hospital in Telangana. The study was done for a period of 6 months from April 2019 to September 2019. Descriptive data was expressed in mean and percentages. Chi- square test was done to assess potential drug-drug interactions and number of medications received by the patients (p<0.05 is considered significant). Results: Polypharmacy was present in 70.75% of elderly patients. The mean drugs prescribed for each patient was 5.7%. Severe potential drug-drug interactions were present in 16.82% patients. Potentially inappropriate medications were assessed using Beer’s criteria and were present in 35.52% of patients. Conclusion: This study thus highlights the importance of reducing the practice of polypharmacy by the treating physician to reduce morbidity and to decrease economic burden on the elderly...
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Posted by admin on Jan 31, 2024 in |
Objectives: Metabolic syndrome (MetS) is a cluster of several metabolic disorders including hyperglycemia, reduced high density lipoprotein cholesterol (HDL-C), raised triglyceride level in serum, hypertension and abdominal obesity. The independent risk factors of high sensitivity C-reactive protein (hs-CRP) levels may have a significant impact on evaluating the prevalence of MetS components in both men and women. Methods: The present study was conducted at Shri Ramkrishna Institute of Medical Sciences and Sanaka Hospital, Durgapur, West Bengal. The study includes 100 patients with MetS as a case and 100 healthy volunteers as a control. MetS was diagnosed according to international diabetes federation. Results: In our study the level of hs-CRP is high in metabolic syndrome patients (4.88 μg/ml) compared to control group is (1.699 μg/ml) A statistically highly significant difference was observed among two groups (p<0.000). Conclusions: The level of hs-CRP maybe used as a surrogate marker of chronic inflammation in metabolic syndrome patients and higher levels of the inflammatory marker hs-CRP are correlated with rise risk for development of cardiovascular...
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Posted by admin on Jan 31, 2024 in |
Antimicrobial resistance (AMR) emerged as a major challenge to public health and significantly impacting the global economy. Infections due to multi-drug resistant (MDR) bacteria are difficult to treat and cause financial burden on patients. Environment may serve as a genetic pool of several drug resistant genes and help bacteria to emerge as notorious superbugs. This study aimed to characterize MDR bacterial isolates from environmental samples and comparative analysis of MDR and multi-drug sensitive (MDS) strains for the expression of virulence factors. Twenty six (n=26) different environmental samples (water, soil, air and surface) were collected for the purpose. The antimicrobial susceptibility testing performed using 12 antibiotics belongs to six different antibiotic classes. The potential virulence factors were determined among MDR and MDS isolates. Thirty (n=30) bacterial isolates belong to 13 different genera (Staphylococcus spp., Enterococcus spp., Escherichia spp., Bacillus spp., Acinetobacter spp., Klebsiella spp., Pseudomonas spp., Shigella spp., Enterobacter spp., Citrobacter spp., Streptococcus spp., Streptobacillus spp. and Proteus spp.) were identified. All recovered isolates were susceptible to aminoglycosides. The most...
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Posted by admin on Jan 31, 2024 in |
Background: In this study, Embelia ribes fruit extract phospholipid complex (ERE-PC) was developed with an attempt to enhance the therapeutic effect by improving its dissolution profile and oral bioavailability. E. ribes, an ancient herb possesses anticancer, anti-fertility, antimicrobial and anti-diabetic activities. However, poor aqueous solubility of phytoconstituents of E. ribes, lowers the therapeutic effectiveness of the extract. Objective To prepare phytosomes of Embelia ribes fruit extract (ERE-PC) to improve in-vitro release profile and oral bioavailability. Method: ERE-PC were formulated by solvent evaporation technique using methanol as reaction mixture which aimed for its improved pharmacological activity. The complex formation was confirmed by FTIR, PXRD, DSC and microscopical studies. The prepared complex was evaluated for functional properties such as solubility, dissolution profile and in-vitro cytotoxic activity. Result: The data obtained from FTIR, DSC and microscopic studies confirmed the formation of phytosomal complex. Aqueous solubility of the extract significantly increased from 23.04 ± 0.0023μg/ml to 84.02 ± 0.0036μg/ml in ERE-PC. Particle size and zeta potential of optimized phytosome was found to be...
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