Posted by admin on Oct 31, 2016 in |
Development of microbial resistance leads new drug discovery, modification of antibiotics, administration of two or more antibiotics, use of traditional medicinal plants and their combinations with antibiotics. In the present study, 72 medicinal plants extracts were screened to potentiate the antimicrobial with erythromycin and amoxyclave. Among all the medicinal plant, Colebrookea oppositifolia was selected to analyze the synergistic activity with erythromycin and amoxyclave. The class I synergism (increase in the zone of inhibition) was observed in petroleum ether extract of leaves and inflorescence of C. oppositifolia in combination with amoxyclave with the increased zone from 6±0.2 mm to 8±0.2 mm and 6±0.2 mm to 10±0.2 mm, respectively. The class I synergisms also exhibited in methanol leaf extract of C. oppositifolia in combination with erythromycin and amoxyclave against S. aureus. Interestingly, the methanolic leaf extract (alone) did not show any antibacterial effect against S. aureus, but in combination with erythromycin/amoxyclave enhanced the zone of inhibition from 3±0.1mm to 9±0.2 mm and 5±0.2mm to 9±0.2mm, respectively. Class II synergism (making drug...
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Posted by admin on Oct 31, 2016 in |
Mangifera indica is commonly found in India, which contains exalted medicinal values. TLC bioautography help us to identify the bioactivity of separated molecules in less time. In this study, leaves of Mangifera indica was subjected for various solvent extraction, followed with phytochemical screening and TLC bioautography for antioxidant and antibacterial activity. Column fractionation was done for all the extracts and subjected for spot assay. The plant chosen for this study was found to have antioxidant and antibacterial...
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Posted by admin on Oct 31, 2016 in |
Pathogenic bacterial infections are of serious clinical concern around the world. Indiscriminate use of antibiotics makes this situation beyond control. Hence, microbiologists are trying to find out the new and promising therapies against these bacterial infections. In the present research, combination therapy, which comprises extract of different medicinal plants and cow urine distillate, was used against six pathogenic gram positive bacteria. The efficacy of this combination therapy was evaluated by two methods: agar well diffusion method and XTT-colorimetric method. It has been demonstrated that CUD has profound synergistic effect on the efficacy of plants-derived extracts against the pathogenic bacteria at the specified level. The relative activity of different extracts with CUD as solvent medium was found to be increased (R.A.I= 1.61 to 2.13) against every bacteria of present study. MIC & MBC results were greatly improved (upto 2 to 3 folds) by the combination therapy. The results obtained in the present study could be useful for further research to assess the effect of combination therapy against other pathogenic microorganisms...
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Posted by admin on Oct 31, 2016 in |
Objective: Diabetes mellitus is metabolic disorder and day by day number of people increased across the world. The aim of the present study was to evaluate the antidiabetic and heapatoprotectivity of ethanolic extracts of Coriandrum sativum in alloxan induced diabetic rat and find out the harmonious activity of the extract. Methods: The alloxan (150 mg/kg, i.p) used for induction of diabetes after making diabetic model the ethanolic extract of Coriandrum sativum leaves used at a dose of 200 and 400 mg/kg b.w. respectively for single extract treatment for four weeks including metformin (200mg/kg b.w) as a reference drug. The statistical analyses were carried out by one- way ANOVA with post-hoc by Dunnett’s Multiple Comparison Test. Results: After 28 days treatment the extracts significantly (p<0.001) reduces serum glucose level and recovered liver function tests (p<0.001) (SGOT, SGPT, ALP and bilirubin) as well glycosylated haemoglobin (p<0.001). Histopathological study showed amelioration in alloxan treated groups. Conclusions: The result suggested that anti-antidiabetic and heapatoprotectivity potential of Coriandrum...
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Posted by admin on Oct 31, 2016 in |
Alkaline protease alone account for 60% of the total world enzyme market with its improved properties such as higher optimum temperature and alkaline pH. Alkaline Lonar Lake, situated in the Buldhana District of the Maharashtra State, India, is a unique ecosystem and harbors various haloalkaliphilic bacterial species which produces biotechnologically important thermo-haloalkaliphilic enzymes. The present study deals with the production and partial characterizations of enzyme protease isolated from the extremophilic Lonar Lake bacterium. A total six bacterial strains were isolated on Horikoshi medium from the water, sediment and matt samples collected from Lonar crater. Out of these, one bacterium showing prominent proteolytic activity was characterized on the basis of morphological, cultural and biochemical parameter and identified as Bacillus halodurans (AJ302709) by16S rRNA sequencing. The alkaline protease produced by Bacillus halodurans showed optimum activities at pH 8, at temperature 700C. It proves that the enzyme alkaline protease produced by this bacillus has potential applications in food, pharmaceutical and detergent...
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