Posted by admin on Dec 31, 2016 in |
For the present study thirty male Swiss albino mice were selected from an inbred colony. They were divided into three groups consisting of ten mice in each group. Group I: Sham exposed, Group II: 10 GHz MW (microwave) exposed, Group III: PDE (Prunus domestica extract) +MW exposed. After 30 days of treatment the animals were sacrificed to study alterations in body weights and biochemical parameters in different organs of Swiss albino mice viz. intestine, liver, testis and spleen. MW exposure did not resulted in significant reduction in body weights compared to sham exposed group of mice. PDE supplementation prior MW exposure did not result in any significant weight gain compared to MW exposed group. Biochemical analysis showed highly significant (p<0.001) variations in Lipid Per Oxidation (LPO), Glutathione (GSH) and protein levels which could be ameliorated by supplementation of PDE prior to MW exposure. Exposure to 10 GHz leads to biochemical alterations in different organs studied in mice which can be ameliorated by PDE supplementation. It can be concluded from...
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Posted by admin on Dec 31, 2016 in |
Background: Due to Escherichia coli O157:H7 bacteria’s peculiar biochemical characteristics it is considered an emerging pathogen. The aim of this study is to design, develop and evaluate the efficacy of a single formulation combining adsorptive and anti infective properties in the treatment of diarrhoea caused by Escherichia coli O157:H7 in an animal model. Method: A bilayered tablet of metronidazole and activated charcoal (AC) formulated via direct compression was developed using hydrophilic mucoadhesive polymer xanthan gum in varying concentrations to ensure modified release of metronidazole in the first layer and activated charcoal with microcrystalline cellulose for instant disintegration in the second layer. Escherichia coli infected Sprague Dawley® rats was utilized to evaluate the efficacy of the formulation. Results: Swelling studies reflected an affinity for the polymer to swell in pH 6.8 where drug release and swelling was influenced by ionic strength of the medium owing to the conformational changes in the xanthan gum in this media with an increase in the zeta charge from -28.5 mV to -40.2 mV, drug...
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Posted by admin on Dec 31, 2016 in |
The aim of present study is to formulate and optimize the site specific controlled release formulation for release of loratadine in upper gastrointestinal tract. Superposrous hydrogel hybrids (SPHH) were prepared by using ferric chloride as crosslinking agent and sodium carboxymethyl cellulose as composite agent. The Superporous Hydrogels were evaluated for swelling ratio, mechanical strength, density, porosity, scanning electron microscopic studies. Statistical software, Design Expert was used for optimization and chosing the final formulation. Loratadine hydrochloride was loaded in optimized formulation and characterized by Fourier Transform Infrared Spectroscopy, X-Ray Diffraction, Differential Scanning Calorimetry and in vitro drug release studies. Superporous Hydrogel Hybrids were prepared with desired mechanical strength and sufficient swelling ratio. Equilibrium Swelling Ratio of hydrogels followed the pattern SPHH < SPHC < CSPH. The pattern may be attributed to the additional crosslinking and decreased poresize. Reduced swelling ratio may be because of restricted polymeric chain flexibility. H- bonds between poly (AM-co-AA) and NaCMC reduced the polymer ability to form H-bonds with water molecules, limiting water absorption. Increase in...
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Posted by admin on Dec 31, 2016 in |
A simple, selective, rapid, precise reverse phase high pressure liquid chromatographic method has been developed for the simultaneous estimation of Hydrochlorothiazide, Amlodipine besylate and Telmisartan in tablet dosage form as per ICH guidelines. The wavelength selected for the analysis was 239 nm for amlodipine besylate, telmisartan and hydrochlorothiazide. The solvents used were methanol and distilled water. The mobile phase 0.05M Potassium dihydrogen ortho-phosphate (pH-3.2), acetonitrile and methanol in the ratio 45:45:10 was used to carry out the separation. Column used was Supelco C18 (250 x 4.6 mm, 5μ). Flow rate was selected as 1ml/min. Retention time for Hydrochlorothizide (HCT), Amlodipine besylate (AML) and Telmisartan (TEL) was found to be 2.9min., 5.1min., 8.2min. The developed method was validated for accuracy, precision, linearity, robustness, ruggedness, specificity. The proposed method can be used for the estimation of these drugs in combined dosage...
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Posted by admin on Dec 31, 2016 in |
Awareness of reproductive health is rights of humans and lack of accurate notification will lead to numerous complications.Pre-marriage is one of the best times to training of these issues. Therefore, this study was done in order to evaluation the effects of premarital education on the girls’ awareness about reproductive health. In this semi-experimental study, 152of womens that participated in courses at marriage counseling center were selected with consecutive sampling method in Garmsar city, Iran in 2012.Data were collected by a questionnaire containing demographic (contains two sections: demographic characteristics and reproductive health) in two stages of pre and post of test. Data were analyzed using SPSS software. According to the findings of this research, the average of girls’ age was 22.41± 3.22 and 55.3% of them, had college education and 53.9% of them were housewives. The mean score of awareness about reproductive health before the education, was 12.81±33.3 that increased to the 17.81±. 2.587after training and this difference was statistically significant (p= 0.000).As well as level of satisfaction of participants...
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