Posted by admin on Sep 30, 2016 in |
The worldwide prevalence of diabetes mellitus (DM) has risen dramatically over the past two decades; based on current trends, more than 360 million individuals will be having diabetes by year 2030. The present study was designed to investigate the hypoglycemic of effect of ethanolic extract of leaves of Passiflora insarnata linn. in streptozotocin-induced diabetes in rat. The pharmacological investigation suggested that the ethanolic extract of the leaves was beneficial in controlling the blood glucose levels, improves the lipid metabolism and prevents diabetic complications from lipid peroxidation in experimental diabetic rats. From this study, it can be concluded that the ethanolic extract of the plant exhibited protective and ameliorative effects against streptozotocin induced pancreatic cytotoxicity and severe hyperglycaemia by enhancing the peripheral utilization of glucose, correcting the impaired liver glycolysis and limiting gluconeogenic formation and also repairing and rejuvenating the residual beta cell population. These effects may be due to the presence of phenolic compounds, flavonoids, and other phytochemical constituents, which could act synergistically or independently in modulating the activities...
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Posted by admin on Sep 30, 2016 in |
The current work on impurity profiling of Sitagliptin in its bulk drugs, included development of a stability- indicating reverse-phased liquid chromatographic method and its validation for the estimation of degradation related impurities (DRIs). Sitagliptin was subjected to acid and alkaline hydrolysis, H2O2 oxidation, thermal degradation and photolysis. Acid hydrolysis and H2O2 oxidation yielded significant degradants, which were isolated on a semi-preparative high performance liquid chromatography (HPLC) and characterized with the help of high-resolution mass spectrometry, 1H-NMR spectroscopy. Possible structures of the DRIs were reveled with the help of mass measurement and NMR spectroscopy. The chromatographic separations were accomplished on Waters Cosmosil C18 column (250 mm x 4.6 mm; 5 µm) using 5mM ammonium acetate and methanol as a mobile phase with gradient elution at 1.0 ml/min flow rate, and eluents were detected using photo diode array detector at 268 nm wavelength. The method was validated with respect to accuracy, precision, linearity, robustness, and limits of detection and quantification as per International Conference on Harmonization (ICH)...
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Posted by admin on Sep 30, 2016 in |
In vitro antibacterial activity of Bryophyllum pinnatum (in vivo generated and in vitro regenerated roots) were analysed in this study. Traditional uses for these plants treat specific conditions or diseases. The present study examined the antibacterial activity using the disk diffusion method using Ethanolic & Methanolic extract as part of the process of understanding the chemistry, toxicity and efficacy of these plant extracts. Methanolic and Ethanolic extracts of this plant was examined using a standard antimicrobial disk diffusion method. Extracts were tested against both Gram negative (Escherichia coli, Vibrio cholerae, Salmonella typhi and Pseudomonas aeruginosa) & Gram positive (Bacillus cereus & Staphylococcus aureus) bacteria. Methanolic in vitro regenerated roots showed higher inhibition zone against all bacterial species while natural growing plant root extract showed minimum inhibition. In vitro regenerated root extract showed moderate inhibition zone against all bacterial species. S. aureus showed maximum (25.2 mm) inhibition zone in in vitro root methanolic extract while V. cholerae showed minimum (14 mm) in natural growing root extract. In ethanolic extract maximum...
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Posted by admin on Sep 30, 2016 in |
Soybean is one of the highest isoflavones source. Isoflavones are one type of phytoestrogen that have a chemical structure similar to estradiol, that’s why it can be used not only to inhibite but also to prevent many symptoms related deficiency of estrogen. However in the small intestine, isoflavones like genistin will be hydrolyzed by β-glucosidase and produce aglycone like genistein, this process can be optimized by hydrolysis with enzyme or chemical. The aim of this study was to obtain the extract of soybean that hydrolysed by hydrochloric acid and to obtain scientific data about the content of genistein in the extract by High Performance Liquid Chromatography (HPLC). Analysis of samples by HPLC using C18 reverse phased column. Genistein standard with a concentration 8 ppm, 10 ppm, 12 ppm respectively diluted with methanol: water (8:2) as much as 5 ml. Soybean extract, weighed as much as 3 mg and dissolved in 10 ml of methanol: water (8: 2). Standard and sample then analyzed with the mobile phase used was methanol:...
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Posted by admin on Sep 30, 2016 in |
The aim of this research work was to formulate and optimize a floating drug delivery system of famotidine. In the present study, preparation of famotidine floating beads, in-vitro evaluation of Floating Drug Delivery System (FDDS), prediction of the release, and optimization of polymers ratio to match target release profile was investigated. An attempt was made to prepare oil entrapped floating beads of famotidine prepared by inotropic gelation method using Sodium alginate (SA), Tamarind seed powder (TSP) where SA & TSP ratio was 95:5 respectively, Span 80 with Calcium Chloride as curing agent and various concentration of Sunflower oil as a floating agent. Size of all the beads was found spherical and uniform and percentage yield of all formulation was found approximate 85%. It was observed that famotidine release from uncoated beads in a considerable “burst” during the first 30 min, due to rapid water ingress and creation of aqueous channels, which suggested that drug release was dependent on dissolution and diffusion of the drug through the polymer matrix. Buoyancy...
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