Posted by admin on Apr 30, 2024 in |
A new HPLC method has been developed and validated for the simultaneous estimation of Rifampicin and Isoniazid, offering a reliable and efficient approach for their analysis. The method employs a mobile phase composed of pH 3.5 phosphate buffer, methanol, and water in a 45:30:25 ratio. It demonstrates a linear response for both drugs within specific concentration ranges, allowing for accurate quantification. Detection is performed at 239 nm, with retention times of 2.8 minutes for Rifampicin and 3.7 minutes for Isoniazid. Accuracy studies have shown excellent recovery rates within ICH guidelines, ensuring the reliability of the results. Notably, the method exhibits high precision with RSD values within acceptable limits, indicating its reproducibility. It has also been found to be simple, rapid, robust, and reproducible, making it well-suited for routine use. This validated HPLC method holds significant potential for both pharmaceutical and clinical applications, enabling accurate and efficient analysis of Rifampicin and Isoniazid in bulk and tablet dosage...
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Posted by admin on Mar 31, 2024 in |
Background: Fatigue is a common symptom in patients with advanced kidney disease, with implications for quality of life and clinical outcome. Fatigue is one of the most frequent complaints of dialysis patients and is associated with impaired health related quality of life (HRQOL). Aim: To monitor and measure the severity of fatigue in dialysis and non-dialysis patients of chronic kidney disease. Methodology: This prospective study was performed in the Department of Nephrology in SVIMS, Tirupati, over a period of 6 months. In this study, Fatigue Severity Scale was used with help of questionnaire form. Results: A total of 30 patients were recruited in this study based on their type of dialysis and we categorized subjects based on Gender, Age, Occupation, CKD Stage, dialysis per week, family history, complications and duration of CKD. We found that average fatigue score in Non-Dialysis subjects is 4.80±1.989, Hemodialysis subjects is 4.20±1.033, Peritoneal dialysis is 2.80±1.476. Conclusion: Our study concludes that patient having comorbidity condition like only Hypertension and Hypertension with Diabetes mellitus were...
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Posted by admin on Mar 31, 2024 in |
Sarcocephalus latifolius have a wide range of medicinal applications. This study evaluated the neurobehavioural activity of crude Leaf extract of Sarcocephalus latifolius on wistar rats and the effect on the histoarchitecture of the temporal lobe. Sixteen adult Wistar rats were divided into four groups of four rats each. Group A served as the negative control and received distilled water, B and C served as the experimental group and received 100mg/kg and 150mg/kg of the extract, while D served as the positive control and received 2.5mg/kg of diazepam. The administration lasted for two weeks and the neurobehavioural activity of the extract was explored using elevated plus maze. After the test the rats were sacrificed and the brain harvested, fixed and processed using normal histological techniques. In the elevated plus maze, the experimental group showed increased open entry, when compared to the negative control but less than the positive control, which is a measure of its anxiolytic activity. The extracts altered the microanatomy of the temporal lobe, in dose dependent manner....
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Posted by admin on Mar 31, 2024 in |
The aim of the article is to formulate and evaluate emulsion also perform comparative study with marketed formulation. Emulsion is a heterogeneous system consisting of at least one immiscible liquid dispersed in another in the form of droplets. Emulsion is prepared with castor oil and liquid paraffin. One marketed formulation was taken for comparison. Identification tests, evaluation tests and stability studies were performed. The emulsion was evaluated by the parameters like pH, viscosity and organoleptic properties. The stability study was performed by testing pH and viscosity. The results of the study showed the good organoleptic properties, pH,...
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Posted by admin on Mar 31, 2024 in |
A simple, sensitive, robust and specific high performance liquid chromatographic (HPLC) method was developed and validated for the simultaneous determination metformin and levothyroxine in human plasma. In the current study, the analysis was performed on Phenomenex Luna 5µ C18 100A (250 x 4.6mm, 5 micron) column using acetonitrile: methanol: 0.03M phosphate buffer (pH 2- 3.5) 50:10:40 v/v/v as mobile phase at flow rate 1.0 mL/min. The analytes were monitored with UV-PDA detector at 251nm. In this developed method Metformin and Levothyroxine elutes at a retention time of 2.698 and 5.929 min respectively. The proposed method is having linearity in the concentration range from 5 to 50μg/mL of Metformin and Levothyroxine. The current method was validated with respect to linearity; precision, lowest limit of detection (LOD) and lowest limit of quantification (LOQ), accuracy and recovery according to the USP guidelines. The system consisted of a pump (Shimadzu, prominence, HPLC), with 20µl sample injector, along with a PDA detector at a wavelength of 251nm. Data was compiled using Shimadzu LC Solution...
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