Posted by admin on Nov 30, 2023 in |
Valsartan is a selective angiotensin type I receptor agonist that has been used in the treatment of hypertension disease. The development of a thermodynamically stable and infinitely dilutable nanoemulsion (O/W) of valsartan with surfactant at a low concentration that can enhance its solubility and stability came about as a result of our investigation into the effectiveness of nanoemulsion as a carrier vehicle for Valsartan delivery in this study. Using the water titration methods, by adjusting the ratio of oil, surfactant, and co-surfactant for carrying out the nanoemulsion region, a pseudo-ternary phase diagram was constructed. For each phase, multiple concentrations of oil and surfactant were combined in variable amounts. After gradual titration with water phase utilizing a vortex mixer for each ratio of oil, surfactant, and co-surfactant (Smix), the translucent and transparent nano-emulsions were discovered. The optimization of surfactant and cosurfactant was done using a pseudo ternary phase diagram which were showing large covered area. According to the study, particle size, polydispersity index, zeta potential, physical stability, and shape were...
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Posted by admin on Nov 30, 2023 in |
This article discusses the assessment of kidney function in patients with coronavirus-associated nephropathy through Doppler ultrasound of renal vessels. The study focuses on research groups comprising individuals affected by this nephropathy. Dopplerography studies aim to evaluate the effectiveness of N-acetylcysteine in treating coronavirus-associated nephropathy. Doppler ultrasound helps assess renal blood flow and vascular parameters, providing insights into the functional state of the kidneys. The evaluation of N-acetylcysteine’s effectiveness suggests an exploration of its potential as a therapeutic intervention for coronavirus-associated nephropathy. This research contributes to understanding the renal implications of COVID-19 and potential treatment avenues through the application of Doppler ultrasound...
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Posted by admin on Nov 30, 2023 in |
The quest for new antimitotic drugs is an ongoing process where microtubules act as an attractive target for anti-cancer drugs. In this work, we report on the anti-cancer activity of Amlodipine besylate (AB), a salt derivative of the dihydropyridine groupdrug Amlodipine, a long-acting third-generation calcium channel blocker. Cytotoxicity assay revealed that AB demonstrated cytotoxic effects against cancer cells with greater specificity against cervical cancer cells (IC50 10 μM) whereas the non-cancerous NKE cells remained unaffected at this dose. AB inhibited HeLa cell migration as observed via scratch assay. Docking studies indicated that AB has the highest binding affinity for vinca site compared to Tubulin’s taxol, colchicine, and laulilamide binding domains. AB similarly interacted with tubulin to vinca and established two hydrophobic interactions with VAL353, PRO325; hydrogen bonds with ASN329, VAL175 and THR218 at the binding pocket. The stability of the Tubulin-AB complex was analyzed by molecular simulation studies. Further AB treated cells revealed altered microtubule architecture and drug-induced microtubule depolymerization in HeLa cells. More detailed study might unravel additional...
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Posted by admin on Nov 30, 2023 in |
Objectives: The present study aims to develop a simple, precise, accurate, and reproducible spectrophotometric Second order derivative method for estimating Favipiravir in bulk and pharmaceutical dosage form. Method: Acetonitrile was used as an organic solvent. The maximum absorption of the drug was found to be at 323nm. The developed method was validated and applied for the estimation of Favipiravir by using a spectrophotometric second-order derivative. Results: The linearity was established in the 5 to 25µg/ml concentration range for favipiravir with a correlation coefficient (r2) of 0.9991, respectively. Percent recovery studies were obtained in the range of 99.55-101.25%. Both interday and intraday precision was found to be satisfactory. The limit of detection and quantitation were found to be 1.5µg/ml and 1.2µg/ml, respectively. The method was statistically validated according to ICH guidelines for linearity, accuracy, and precision. The relative standard deviation was found to be less than 2%. Conclusion: The proposed method is simple to use, affordable and cost-effective. It can be successfully applied to estimate favipiravir in bulk and pharmaceutical...
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Posted by admin on Nov 30, 2023 in |
An attempt was made to identify actinomycete strains of the South coastal region of Andhra Pradesh, India along with the study of their antimicrobial potential. Among the 50 actinomycete strains isolated, one strain (VLS-2) showed strong antimicrobial activity against Gram-positive and Gram-negative bacteria as well as fungi. It was identified as Kocuria assamensis based on morphological, biochemical, physiological as well as molecular characteristics. ISP-1 medium supplemented with sodium chloride @ 7% maintained at pH 7.0 supported the maximum yield of secondary metabolites by the strain when incubated at 35oC for six days. Production of antimicrobials by the strain varied significantly with different carbon and nitrogen sources. The present study reveals the potentiality of actinomycete strains isolated from South-coast of Andhra Pradesh, India which shows further scope to investigate their novel bioactive compounds by employing both natural product chemistry and modern biotechnological...
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