Posted by admin on Sep 30, 2016 in |
Dillenia indica linn fruit, commonly known as elephant apple has indigenous use in various culinary dishes of Assam and it has a history of being used as traditional medicine. The present study was undertaken to evaluate its antioxidant and chemopreventive properties. Ethanolic extract of Dillenia indica fruit was used as experimental sample. Antioxidant activity was studied in vitro, at concentrations (20µg/ml, 40µg/ml ,60µg/ml, 80µg/ml, 100µg/ml) where it showed significant (p<0.05) inhibition of free radicals formation, hydroxyl radical and nitric oxide scavenging property and also reducing ability when compared with a standard antioxidant, ascorbic acid. Chemopreventive potential was evaluated in 8-10 weeks old swiss albino mice by giving a single tropical application of initiator- 7,12-dimethylbenza(α)anthracene (DMBA) at a concentration of 100µg/100µl acetone and promotion after 14 days by repeated application of croton oil (1% in acetone) thrice a week for 15 weeks. Oral application of D.indica at dose of 250 mg/kg body weight of mice selected after acute oral toxicity test, showed significant (p<0.05) reduction in DMBA induced mice skin...
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Posted by admin on Sep 30, 2016 in |
Bromhexine hydrochloride, mucolytic agent used in the treatment of respiratory disorders. Azithromycin is use in wide variety of bacterial infections. The aim of this present work is to formulate dual drug delivery system containing azithromycin as immediate release and bromhexine hydrochloride as sustained release action. The sustained release part as core tablet of bromhexine prepared because of the frequent daily dosing is required for therapy. The floating formulation of core tablet was prepared because it dissolved in the pH range of 1 to 4. The core tablets of bromhexine hydrochloride were prepared by wet granulation technique. The prepared core tablets were evaluated for different parameters for selection of formula for floating the tablets. The core compressed tablets were evaluated for quick release of coat layer containing disintegrants. The various formulations of core tablets were prepared using HPMC K4M and K15M in different concentration. The prepared tablets were evaluated for physical parameters, disintegration time, floating lag time and drug release study. All the batches show around 70 sec disintegration time,...
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Posted by admin on Sep 30, 2016 in |
Asthma is obAdd Newstructive disease characterized by bronchoconstriction and inflammation for which beta 2 (β2) agonists that act through the β2 adrenoceptor are the first line agents of choice. Glycine16Arginine (Gly16Arg) is a common single nucleotide polymorphism (SNP) of β2 adrenoceptor whose effects on treatment response remain inconclusive. Hence the study aims to understand and determine the effect of the Gly16Arg SNP on β2-agonist binding and treatment responsiveness. Structure guided mutagenesis was performed with discovery studio tool, energy minimization was performed using Chemistry at Harvard Macromolecular Mechanics(CHARMM) force field, protein confirmation was studied using Ramachandran plot, Molecular docking analysis was performed using Autodock 4.2 and statistical analysis was performed using Graph pad prism 6.0. Statistically significant difference was observed between the binding energies of Glycine16 (Gly16) and Arginine16 (Arg16) groups. Further, the binding energies of β2 agonists were found to be comparatively less in Arg16 group than the Gly16 group suggesting that the Arg16 variant carriers may be poor responders of β2 – sympathomimetic therapy. Presence of functional non...
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Posted by admin on Sep 30, 2016 in |
The purpose of this study examine whether the polymorphism in p53 upregulated modulator of apoptosis (PUMA) gene consequential treated with purified Nigella sativa lectin in tumor induced mice. Nigella sativa (N. sativa) is a widely used medicinal plant throughout the world. Possess a seed lectin that was purified by combination of ammonium sulfate precipitation and affinity chromatography on a Sepharose 4B column. The Nigella sativa strongly agglutinated human erythrocytes. The hemagglutinating activity of lectin was maintained after incubation at a wide range of temperature and pH and also was independent of divalent cations. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, lectin exhibited an electrophoretic profile consisting of a single band with apparent molecular mass of 45 kDa. Experimental groups were organized into 6 groups including 10 animals per each, additionally control group, blood samples were collected form mice before and after lectin treatment for liver and kidney enzymes biochemical analysis, Data statistical analysis for mitochondrial enzymes in our study showed a very highly significant (P>0.001) difference in the mean value...
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Posted by admin on Sep 30, 2016 in |
Thiamine hydrochloride, also known as Vitamin B1, is a water-soluble vitamin which is important biologically and pharmaceutically. A new simple and sensitive spectrophotometric method has been developed for the determination of Thiamine Hydrochloride (Vitamin B1) in pure form and biological fluids. The method is based on the diazotization reaction of Thiamine Hydrochloride with Procaine HCl which reacted with sodium nitrate and hydrochloric acid to form a red water-soluble azo dye in basic medium, that has a maximum absorption at λmax = 507 nm. Beer’s law is obeyed over the concentration range (2-60 µg.ml-1), the molar absorptivity of 4.047×104 L.mol-1 .cm-1 and Sandell sensitivity of 7.417×10-3 µg .cm-2 and the method have an accuracy and precision between (0.02-0.16) while the relative standard deviation between (0.805-2.049). The structure of dye showed that (1:2) product was formed between the drug and diazotized reagent. The method was applied successfully for the determination of Vitamin B1 in pharmaceutical preparations and biological samples. The results showed that the concentration of vitamin B1were decreased, in both...
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