Posted by admin on Nov 30, 2016 in |
Objective: The aim of the present study was to evaluate the clinical and radiographic aspects of pulpal and periradicular tissue for patient undergoes endodontic treatment. Patient and method: The present study was limited to patients who treated endodontically by postgraduate students of Conservative Department, School of Dentistry/Faculty of Medical Sciences between January 2014 to February 2015. A total number of 174 case sheets of patients age ranged from 16 years to 53 years with the mean age being 31.1 years and (92) male and (82) female. The data were categorized according to two parameters, clinical and radiographical evaluation of pulpal and periradicular tissue. Results: Out of the 174 patients who were included in the study, symptomatic irreversible pulpitis (44.25%) was the most common clinical diagnostic disease of pulpal tissue and (45.97%) of cases undergo root canal therapy had normal periradicular tissue. Regarding radiographic assessment of pulpal and periradicular tissue, all criteria (alveolar bone, lamina dura, roots, pulp chamber and pulpal canal) are within the normal limit. Conclusion: Symptomatic irreversible...
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Posted by admin on Nov 30, 2016 in |
Objective: The purpose of this study was to investigate the prevalence of non-polio enteroviral infections by quantitative and qualitative PCR assays in neonates 3-30 days old with clinical diagnosis of sepsis. Methods: The patients included all the neonates aged 3-30 days admitted with diagnosis of neonatal sepsis. Blood specimens, throat swabs, and in 60 cases CSF specimens were tested for Enteroviruses (EVs), using a quantitative RT-PCR assay. Alternate RT-PCR assays separated polio and non-polio enteroviruses. Demographic data including age, sex, type of feeding and gestational age were obtained. Results: As PCR results revealed, of the 177 neonates with clinical diagnosis of sepsis, one patient (0.55%) was positive for non-polio EVs in all of throat, serum and CSF samples. In 3 patients (1.7%), the results were positive for non-polio EVs in both throat and serum, while in15 (8.45%) others only throat samples were positive. Sex, age and gestational age had no significant relationship with the prevalence of the infections statistically (P>0.05). The prevalence of non-polio EV infections ranged from 6.1%...
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Posted by admin on Nov 30, 2016 in |
In the present study, the various extracts viz aqueous, ethanol, chloroform and Petroleum ether of Biophytum sensitivum whole plant were evaluated for the anticancer activity against cancer cell lines. The cervical (Hela) and liver cancer (HepG2) cell lines were used to screen the in vitro anticancer challenging with the B.sensitivum extracts. Concentration ranges from 20 to 100 microgram of B.s crude extracts were screened against Hela and HepG2 cells. The antiproliferative activity of B.sensitivum were performed by 3-[ 4,5–Di methyl thiazol-2-yl]-2, 5-diphenyltetrazolium Bromide (MTT) assay. The cytotoxicity was highly significant in the ethanolic extract and chloroform extract than the aqueous and petroleum ether extract. The maximum non toxic concentration was observed at 150µg concentration of aqueous extract in the vero cell...
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Posted by admin on Nov 30, 2016 in |
A new RP-HPLC method was developed for Mephenesin (MEP) and Ibuprofen (IBU) and validated as per ICH guidelines. Good chromatographic separation of Mephenesin and Ibuprofen was achieved by using Agilent C18 column (150mm×4.6mm, 3.2µ p.s). The system was operated at ambient temperature using a mobile phase consisting of Acetonitrile, 0.01M potassium dihydrogen phosphate pH 3.0 (60:40 v/v) isocratically at a flow rate of 0.5 ml/min. Detection was carried out at 212 nm and retention time was 3.4 min and 9.3mins respectively. Linearity was achieved from 10-40 µg/ml for Mephenesin and 2-8µg/ml for Ibuprofen with r2>0.99. The analytical method validation studies were performed as per International Conference on Harmonization-Quality (ICH-Q2 (R1)) guidelines. The method was efficiently validated with acceptable accuracy, specificity and precision for the estimation of Mephenesin and ibuprofen. It also aimed to apply the developed and validated method, for the analysis of drug release studies in marketed formulation (gel X) containing Mephenesin and...
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Posted by admin on Nov 30, 2016 in |
The present study is an attempt to formulate and evaluate cocrystals of poorly water soluble drug Darunavir by cooling crystallization method using succinic acid as coformer. Cooling crystallization method used to prepare the cocrystals of darunavir with succinic acid. The prepared cocrystals were characterized by Microscopic characters, Product yield, Surface morphology. (SEM), Differential scanning calorimetry, X-ray diffraction, Fourier Transform Infrared Spectroscopy, Micromeretic properties, Dissolution study of cocrystals, stability studies, Thus, cocrystals using water soluble polymer like Succinic acid can be prepared using cooling crystallization method for a poorly water soluble drug such as...
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