Posted by admin on Sep 30, 2016 in |
A mononuclear copper(II) complex [Cu(bpy)2(NO3)] NO3 H2O (bpy = 2,2’-bipyridine) has been synthesized and characterized based on physico-chemical spectral data. The complex is characterized based on electronic, IR and ESR spectroscopies. The structure of the complex is determined by single crystal X-Ray crystallography. The complex crystallizes in monoclinic space group P-1. Structural analysis reveals that the complex adopts square pyramidal geometry with CuN4O chromophore. The interactions of these complexes with calf thymus DNA have been investigated using absorption spectrophotometry. The high binding constant 4.89 x 106 M–1 may be due to the strong electrostatic attraction between the cationic complex and the negatively charged phosphodiester backbone of DNA. Nuclease activities of complexes are investigated on double stranded pBR322 plasmid DNA using gel electrophoresis experiments under different conditions. The complex cleaves DNA more effectively in the presence of...
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Posted by admin on Sep 30, 2016 in |
Most low density bags are made from polythene, a chemically inert compound consisting of carbon and hydrogen. Burning of this plastic waste and burying of the plastics releases harmful toxic material which is a major pollutant in environment. Degradation of waste plastics through microorganism is the alternative method to deal with such problems. In this present study low density polythene bags degrading bacteria were isolated from greater Visakhapatnam dumping yard. Out of 20 isolates, one isolate was selected, due to its significant activity in biodegradation of polymer. After 30 days of incubation the isolate resulted 28% in weight loss of polythene bags. SEM images of the treated polythene bags confirmed that the isolate is having significant biodegradation ability by forming holes on the surface. Increased biomass of the bacteria on polymer and decreased biomass in spent medium indicates the significant utilization of polythene, as carbon source. The degraded material contains 5×102 CFU/mL of bacteria. Morphology and biochemical tests confirmed that isolate belongs to Pseudomonas sp. This work reveals that...
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Posted by admin on Sep 30, 2016 in |
Simple and sensitive extractive spectrophotometric methods for the determination of Ethambutol in pure form and in pharmaceutical formulations using triphenyl methane dyes have been developed. The developed methods involve formation of coloured chloroform extractable ion-pair complexes of the drug with triphenyl methane dyes viz., bromocresol green (BCG), bromocresol purple (BCP) and bromophenol blue (BPB) in acidic medium. The extracted complexes formed with BCG, BCP and BPB showed absorbance maxima at 420, 419 and 415 nm respectively. The stoichiometry of the ion- pair complex is found to be 1:1 in each case. Beer’s law is obeyed in the concentration ranges 2.0-25, 3.0-30 and 4.0-40 μg/ml with BCG, BCP and BPB respectively. The effect of concentration of dye, pH, and interference of excipients have been studied for optimization. The limits of detection and quantification have been determined for all the three methods. These methods have been validated as per the guidelines of ICH. The results of analysis were validated statistically through recovery...
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Posted by admin on Sep 30, 2016 in |
Background: Diabetes mellitus comprises a group of metabolic disorders that share the common phenotype of hyperglycemia, association with the biochemical alteration of glucose and lipid peroxidation. Abnormalities in metabolism, including elevated polyol pathway, increased non-enzymatic glycation, accumulation of AGEs, uncontrolled oxidative stress, protein kinase C activity (PKC) and the expression of vascular endothelial growth factor (VEGF) result from glucose dysmetabolism and evidently also contribute to the development of retinopathy. The aim of this study was to analyze and correlate determine the levels of Malondialdehyde and Superoxide dismutase in patients of Type 2 diabetes mellitus with and without retinopathy. Materials and Methods: The study population comprised of 54 type 2 diabetics with retinopathy and 54 type 2 diabetics without retinopathy in the age group of 35-74 years. HbA1c, MDA, and SOD were assayed for both subjects. Results were analyzed carried out by using SPSS 16.0 version (Chicago, Inc., USA). Results: Serum MDA levels were highly significant increased in Type 2 Diabetes Mellitus with Retinopathy in comparison to without Retinopathy (4.25±1.03...
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Posted by admin on Sep 30, 2016 in |
The present study was carried out to evaluate anti-tussive activity of ethyl acetate and methanolic extract of leaves of Adhatoda vasica Nees. As cough is a natural reflex expulsive defense mechanism of the body, it is the most common symptom of respiratory disease. Ammonium hydroxide and Sulphur dioxide induced cough models in mice were used for evaluation of antitussive activity of ethyl acetate and methanolic extracts of leaves Adhatoda vasica. The ethyl acetate and methanolic extract of leaves Adhatoda vasica was orally administered at the dose levels of 500 mg/kg b.w. showed maximum inhibition of cough by 82% and 81% respectively .The standard anti-tussive drug Codiene phosphate (10mg/kg b.w.) showed maximum inhibition of cough by 84%. It was found that both extracts of Adhatoda vasica showed anti-tussive activity and obtained percentage inhibition of cough reflex is approximately comparable as standard...
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