Posted by admin on Nov 30, 2016 in |
The antimicrobial effects of I. balsamina seed hexane extract against Staphylococcus aureus, Klebsiella pneumonia, Proteus vulgaris, and Serratia marcescens were determined using the standard technique. As well as the phytochemical properties of the extract on the test isolates were also examined using the standard methods. The phytochemical components of the hexane extract of the I.balsamina seed include falvonoids, alkaloids, phenol, tannin, alkaloids, steroid, saponin, phenol, flavonoids, triterpenes glycosides and carbohydrate. All the test organisms were susceptible to 25mg/ml of the extract. The MIC and MBC of the hexane extract of the I.balsamina against S. aureus and E. coli was 50mg/ml, while that of K. pneumonia and S. marcescens were 75mg/ml and 100mg/ml of the extract respectively. The result of this study suggests that the hexane extracts of I. balsamina could be suitable for the treatment of diseases infections caused by S. aureus, K.pneumonia, P. vulgaris and S....
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Posted by admin on Nov 30, 2016 in |
Metal nanoparticles are usually synthesized using various chemical methods such as chemical reduction, Solvo-thermal reduction, electrochemical techniques and photochemical reaction in reverse micelles. All these methods are divided into two categories chemical and green chemical. Each having certain advantages and disadvantages. In this study we have tried to give a few examples of these methods for the synthesis of Titanium dioxide nanoparticles and further discussed to compare the...
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Posted by admin on Nov 30, 2016 in |
Aim of the current study was to evaluate the efficacy of non polar extract (NPE) of Aloe vera gel (AVG) for 60 days (~25μg/daily/orally) in rodent model of polycystic ovarian syndrome (PCOS). After treatment, steroid hormones and insulin levels were estimated by ELISA, serum lipid profile along with ovarian steroidal enzyme activity. Also, transcript as well as protein level of key receptors and steroidogenic enzymes were evaluated. Parallely, pairs of ovaries were excised and stained with Hematoxylin and Eosin (HE) to monitor the structural changes from group of animals. During course of study, stability of non polar extract (NPE) of AVG was evaluated by High Performance Thin Layer Chromatography (HPTLC) during study (15, 30, 45, 60 days). PCOS rats after treatment with NPE demonstrated a decrease in serum testosterone and insulin level with improved estradiol and progesterone levels. Also, decrease in transcripts level of steroid receptors while increased aromatase expression were observed after NPE treatment. These genes expression study was correlated with relative changes were observed with protein expressions....
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Posted by admin on Nov 30, 2016 in |
The aim of this work is to prepare self-micro-emulsifying drug delivery system (SMEDDS) for oral bioavailability enhancement of a poorly water soluble drug, Pitavastatin. Solubility of Pitavastatin was determined in various vehicles. SMEDDS is mixture of surfactants, oils and co-surfactants, which are emulsified in aqueous media under conditions of digestive motility and gentle agitation that would be take place in the gastro-intestinal (GI) tract. Pseudo-ternary phase diagrams were made to detect the efficient self-emulsification region and particle size distributions of the resulting micro-emulsions were resolute using a laser diffraction sizer. Optimized formulations for in vitro dissolution and bioavailability assessment were Capmul PG 8, Tween 80 and Transcutol P. The release rate of Pitavastatin from SMEDDS have significantly higher than the conventional tablet. These suggest the superiority of prepared SMEDDS of Pitavastatin calcium for In-vitro drug release that may further in enhancement of bioavailability. Our studies illustrated the prospective use of SMEDDS for the delivery of hydrophobic compounds, such as Pitavastatin by the oral...
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Posted by admin on Nov 30, 2016 in |
This study was designed to determine the extent of photolytic degradation of diazepam tablets, without light-protective packaging, available in the market. The UV spectroscopy method was designed and applied for this purpose. A total of 2160 tablets (each tablet contained 5 mg diazepam) of three different brand formulations A, B and C were chosen randomly. These tablets were then exposed to different indirect and direct lighting conditions i.e. normal room light condition (for 60 days), direct sunlight exposure (6 hours per day for 3 days), 25 Watt incandescent light bulb exposure and 40 Watt incandescent light bulb exposure (6 hours per day for 3 days). Samples from these light exposures were analyzed for potency determination using UV spectrophotometer and compared against the tablets kept in dark place (Control). Tablets of all three formulations showed marked degradation. The overall average degradations were 23.64%±5.53%; 28.36%±4.87%; 19.72%±4.83% and 25.40%±4.17% respectively for normal room light, direct sunlight, 25 Watt and 40 Watt incandescent bulbs whereas tablets kept in dark place showed little or...
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