Posted by admin on Sep 30, 2016 in |
Aim of this study was to develop a HPTLC fingerprint profiles of Tinospora cordifolia stems powdered by different procedures viz; Sample 1 (fresh aqueous powder), 2 (freeze drying powder), 3 (aqueous freeze drying powder) and Sample 4 (dry powder). The study also includes qualitative, quantitative secondary metabolites and the antioxidant potential of all the samples. Result of various physiochemical and phytochemical screening shows a variation among all powdered stems. HPTLC profile revealed a similarity between the samples 1, 2 and 3 while sample 4 shows much lesser no. of constituents. TLC profile of all the samples followed a similar trend like HPTLC fingerprinting. Besides this, secondary metabolite profiles viz; total phenolics, flavonoids, proanthocyanidin and anthocyanidin content of these samples reveals that maximum phenolics and flavanoids content in sample 1 (51.5 µg GAE/mg Wt) and 3 (14.0 µg QE/mg Wt). However, sample 4 was greatly enriched with maximum proanthocyanidin (27.7 µg CE/mg Wt) and anthocyanidin (175 µg CE/mg Wt) contents. Antioxidant potential of these powders was evaluated by total antioxidant,...
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Posted by admin on Sep 30, 2016 in |
The seeds of Vitis vinifera have been shown to have antioxidant, anticancer and antimicrobial properties due to the presence of rich bioactive phenolic components like resveratrol. The research deals with the synthesis of silver nanobioconjugates from the methanolic extract of grape seeds and the pure compound resveratrol. The presence of resveratrol was confirmed by HPLC analysis in methanolic extract of various parts of grape fruits such as skin, pulp and seeds of two varieties (pink and black). As higher amount of resveratrol was present in the seed extract of pink grapes, it was selected for the silver nanobioconjugate synthesis. Various conditions were applied to influence the rapid reduction of Ag+ to Agο, such as microwave heating, water bath heating, sunlight exposure and incubation at 37οC for nanobioconjugate synthesis. The reduction of silver ions was confirmed by color changes, adsorption spectroscopy (280-800nm) and percentage yield. Among the four different methods, sunlight exposure proved to be rapid in the synthesis and the yield of nanobioconjugates was also high. Therefore, this method...
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Posted by admin on Sep 30, 2016 in |
The worldwide prevalence of diabetes mellitus (DM) has risen dramatically over the past two decades; based on current trends, more than 360 million individuals will be having diabetes by year 2030. The present study was designed to investigate the hypoglycemic of effect of ethanolic extract of leaves of Passiflora insarnata linn. in streptozotocin-induced diabetes in rat. The pharmacological investigation suggested that the ethanolic extract of the leaves was beneficial in controlling the blood glucose levels, improves the lipid metabolism and prevents diabetic complications from lipid peroxidation in experimental diabetic rats. From this study, it can be concluded that the ethanolic extract of the plant exhibited protective and ameliorative effects against streptozotocin induced pancreatic cytotoxicity and severe hyperglycaemia by enhancing the peripheral utilization of glucose, correcting the impaired liver glycolysis and limiting gluconeogenic formation and also repairing and rejuvenating the residual beta cell population. These effects may be due to the presence of phenolic compounds, flavonoids, and other phytochemical constituents, which could act synergistically or independently in modulating the activities...
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Posted by admin on Sep 30, 2016 in |
The current work on impurity profiling of Sitagliptin in its bulk drugs, included development of a stability- indicating reverse-phased liquid chromatographic method and its validation for the estimation of degradation related impurities (DRIs). Sitagliptin was subjected to acid and alkaline hydrolysis, H2O2 oxidation, thermal degradation and photolysis. Acid hydrolysis and H2O2 oxidation yielded significant degradants, which were isolated on a semi-preparative high performance liquid chromatography (HPLC) and characterized with the help of high-resolution mass spectrometry, 1H-NMR spectroscopy. Possible structures of the DRIs were reveled with the help of mass measurement and NMR spectroscopy. The chromatographic separations were accomplished on Waters Cosmosil C18 column (250 mm x 4.6 mm; 5 µm) using 5mM ammonium acetate and methanol as a mobile phase with gradient elution at 1.0 ml/min flow rate, and eluents were detected using photo diode array detector at 268 nm wavelength. The method was validated with respect to accuracy, precision, linearity, robustness, and limits of detection and quantification as per International Conference on Harmonization (ICH)...
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Posted by admin on Sep 30, 2016 in |
In vitro antibacterial activity of Bryophyllum pinnatum (in vivo generated and in vitro regenerated roots) were analysed in this study. Traditional uses for these plants treat specific conditions or diseases. The present study examined the antibacterial activity using the disk diffusion method using Ethanolic & Methanolic extract as part of the process of understanding the chemistry, toxicity and efficacy of these plant extracts. Methanolic and Ethanolic extracts of this plant was examined using a standard antimicrobial disk diffusion method. Extracts were tested against both Gram negative (Escherichia coli, Vibrio cholerae, Salmonella typhi and Pseudomonas aeruginosa) & Gram positive (Bacillus cereus & Staphylococcus aureus) bacteria. Methanolic in vitro regenerated roots showed higher inhibition zone against all bacterial species while natural growing plant root extract showed minimum inhibition. In vitro regenerated root extract showed moderate inhibition zone against all bacterial species. S. aureus showed maximum (25.2 mm) inhibition zone in in vitro root methanolic extract while V. cholerae showed minimum (14 mm) in natural growing root extract. In ethanolic extract maximum...
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