Posted by admin on Jun 30, 2016 in |
Forensic science is growing rapidly in the world today. During the past ten years, medico-legal investigations highly expanded to include all areas of forensic science. The present aim of project investigated 29 samples of blood and 29 samples of saliva swabs that were collected from human volunteers. The saliva samples were collected by buccal swab but the blood samples were collected by Bode Secur Swab S.I.T. Collector. The experiments were done at four different temperatures (55°C, 37°C, 24°C and 4°C) for 28 days. The results showed that, DNA quantity that was investigated at a temperature of 4°C and 24°C in both blood and saliva samples was more or less remained the same during the whole period of the study, comparing values for day one with all other days including day 28. DNA quantification of human blood following extraction at 37°C was 46.14 ng/μl ± 0.22 at day one then starts to decrease until it reached 36.05 ng/μl ± 0.07 at day 28. In contrast, the result obtained from real-time...
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Posted by admin on Jun 30, 2016 in |
Background: In present study, we aim to assess the anticandidal activity of crude extracts and screening of bioactive compounds and their identification by NMR, IR and GC-MS. Results: A total of 192 isolates were isolated from patients having oral and vaginal candidiasis and a total of 134 isolates showed positive results with Gram staining. The selected isolates APKU-2, APKU-3 and APKU-4 were identified as C.albicans, C.lusitaniae and C.krusei by studying its morphological, biochemical, and molecular characteristics. Chemical characterization of the extracted compounds was done by H1NMR, Fourier transform infrared spectroscopy (FTIR), Gas chromatography–mass spectrometry (GC-MS) analysis to determine the structure, melting point, and molecular mass of the purified compounds. Conclusions: The crude extracts and purified compounds from the sandalwood holds potential as medications for various antifungal therapeutics. However, further research on their potential incorporation into different preparations, safety and cost- effectiveness remains to be...
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Posted by admin on Jun 30, 2016 in |
Mentha piperitais currently one of the most economically important aromatic and medicinal crops in all over the world. Phytochemical investigation of the leaves of Mentha piperita (L.) (Lamiaceae) yielded a new compound, which is decarboxyrosemarinic acid galactoside. Formulate as 3’, 4’-dihydroxy-β-phenyl ethyl caffeate-4-(3’’’-menthyl)-4’-β-D-galacto pyranoside. 13C NMR, 1H NMR, FABMS, IR were used for structural characterization. Wound healing responses of this compound was evaluated from biochemical as well as biophysical parameters and analyze the role of isolated compound. The parameters studied included rate of wound contraction and the period of epithelialization in excision wound model. Tissues were removed on different days of intervals and subsequently analyzed for specific assays Tensile strength in incision wound model was assessed along with histopathological examinations. Compound-1was found to increase the cellular proliferation and collagensynthesis at the wound site, as indicated by increases in amounts of DNA synthesized, protein content. Compound-1treatment was also shown to decrease the levels of lipid peroxides (LPs), while the activity of enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase...
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Posted by admin on Jun 30, 2016 in |
Paracetamol tablets are popular over the counter (OTC) products among the patients as a good analgesics and antipyretics. The objective of this study was to compare the quality of the paracetamol tablet formulations those are locally available in Trinidad & Tobago pharmaceutical market manufactured by various pharmaceutical companies with pharmacopoeia standards. The four popular brands (A, B, C, D) of paracetamol conventional tablet of 500 mg strength were chosen. The paracetamol tablets were obtained from government hospital pharmacies as well as from local private pharmacies. To compare the quality of tablet formulations of different brands various official parameters like friability, weight variation, disintegration time, dissolution and drug assay tests were performed as per the pharmacopoeia. The result of all these parameters of different brands were in the pharmacopoeial limits so it could be concluded that marketed pharmaceutical tablets of paracetamol of these brands are safe, effective and efficacious as well as satisfy quality control limits of...
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Posted by admin on May 31, 2016 in |
Inhibition of viral coating protein is an established therapeutic strategy for the treatment of many influenza treatment regimes. Although resistance to such inhibitors is common phenomena in different influenza treatment, such resistance can be averted by targeting coating proteins with phytochemicals. Swine Flu neuraminidase protein (H1N1) has been currently focused target for influenza inhibitor research. We used computational high throughput screening approach to identify novel phytochemical inhibitors from phytochemical knowledgebase. Our computational method used in this study is an integration of qualitative models obtained from consensus hydrated virtual screening protocols for H1N1 Neuraminidase 1 (N1) to identify the novel phytochemical inhibitors. Using the available N1 crystal structures in protein databank flexibility and hydration states were analyzed and validated. The three representative crystal structures with open and closed conformations with highly conserved waters were used to screened the phytochemical database and identified novel inhibitors, among them a first in class alkaloids inhibitor that have shown better affinity against neuraminidase protein over marketed drugs. Our studies suggest that this computational screening...
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