Posted by admin on Jul 31, 2016 in |
Urinary tract infections causing bacteria play an important role in expression and spread of drug resistance among bacteria in the hospitals and in community. Because of rapid increase in antibiotic resistance, alternative antimicrobial agents are needed to be developed and employed to control multi-drug resistant bacteria. The present study focuses on antibacterial activity of Green and Black tea (Camellia sinensis) extracts against the virulent uropathogens. Twelve bacterial isolates from urinary tract infections were studied for their ability to produce virulence phenotypes like hemolysin, biofilms, gelatinases, hydrophobic nature and penicillinases. Antibacterial susceptibility testing was done. Out of the total isolates tested 67% of E.coli and 60% of Klebsiella spp were multi drug resistant Penicillinase production as tested by filter paper iodometric method and biofilm production on Congo red plates were the virulence features exhibited by maximum number of E.coli and Klebsiella Spp. Among the aqueous, methanol and ethanol extracts tested for antibacterial activity, methanol extracts showed maximum dimeter of zone of inhibition. Minimum inhibitory concentration for ethanol and aqueous extracts was 0.8mg /ml whereas that for...
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Posted by admin on Jul 31, 2016 in |
The aim of my present study is to Develop and Evaluate Microemulsion for topical application of Aceclofenac by using oleic acid at different ratios for the treatment of pain. Microemulsion was prepared by water trituration method using oleic acid as oil phase, tween-80 as surfactant and polyethylen glycol-400 as co-surfactant. Different oils, surfactants and co-surfactants were screened to select ideal components of microemulsions with good solubility and excellent skin penetration of Aceclofenac. The solubility of Aceclofenac was highest in oleic acid followed by olive oil, and isopropyl myristate, isopropyl palmitate. Microemulsion-3 (ME-3) was exhibited 98.54±0.26% higher drug content then other formulations. Among all formulations, the highest permeation flux of µg/cm2/hour was observed in of formulation...
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Posted by admin on Jul 31, 2016 in |
Background: Pharmacists’ jobs in Jordan are important and contribute to the welfare of patients and citizens. Through interviews of a number of pharmacists it was felt that there is need to study their feelings about their rights and satisfaction. Objectives: The aim of this study is to measure the perceptions of Jordanian pharmacists on attaining their rights, and the impact of these rights on their satisfaction. Methods: The study is an observational one. A random sample of 49 pharmacists was chosen to fill a self- administered questionnaire covering the dimensions of pharmacists and their satisfaction. The study tested the following hypothesis: there is no significant impact (at level α=0.05) of Pharmacists’ rights on their satisfaction. Results: The null hypothesis was rejected indicating a significant impact of pharmacists’ rights on their satisfaction. The results also showed that pharmacists were not highly satisfied, whereby they reported satisfaction mean of 2.8 out of 5. Pharmacists’ perception on salary was also low, (mean=2.71). Means and standard deviations of all questionnaire items are reported. ...
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Posted by admin on Jul 31, 2016 in |
Objective: Present study involved comparison of thin layer chromatograms and quantification of valerenic acid (VA) in four morphologically similar rhizome drugs – Valeriana jatamansi, Nordostachys jatamansi, Selinum vaginatum and Ferula sumbul. Materials and methods: Ethanol extract of the four drugs were prepared under standard conditions and VA content was determined using TLC densitometry. Results: Comparative fingerprint chromatograms of the four drugs were developed and mean content of VA was calculated. S. vaginatum contains maximum VA content (360.22 µg/g). Conclusion: The four closely allied drugs could easily be differentiated based on thin layer chromatograms and VA...
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Posted by admin on Jul 31, 2016 in |
An RP-HPLC method to be used to quantitate the amount of a biomarker in the roots of Paullinia pinnata was developed. The root bark was milled and extracted and the dry powder obtained taken through column chromatography (CC). The biomarker was then isolated. The melting point of the biomarker was determined and found to be 117-119 oC. A RP-HPLC method was successfully developed and used to quantify the biomarker in the chloroform extract. The mobile phase used was Methanol:Water (9:1) with an injection volume of 100µl and a range of 1.000. The method employs a Phenomenex Kromasil C-8, 5µ, 250mm × 4.6mm, 100Ǻ reverse phase column at a flow rate of 1.3ml/min, and a wavelength of 210nm. The method gave a retention time of 2.9687 ± 0.2657 (n=15). The percentage content of the biomarker in the root of Paullinia pinnata was determined to be 0.052009±0.004321% w/w (n=12). The HPLC method was validated for linearity, repeatability, intermediate precision, and robustness. The limits of detection and quantitation were also...
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