Posted by admin on Apr 30, 2016 in |
Biodegradable polymeric implant offers a novel approach for sustained drug delivery that provides an option to the patient of avoiding surgical retrieval of implant post-use. Chitosan, a biodegradable polymer extracted from the hard outer skeleton of shellfish, is used nowadays in many pharmaceutical applications (ophthalmic, nasal, sublingual, buccal, periodontal, gastrointestinal, colon-specific, vaginal, transdermal drug delivery and mucosal-vaccine). The main objective of the study was to prepare and evaluate an implantable system of Norethisteron Acetate with chitosan-sodium alginate. Norethisteron Acetate plays an important role in the long term treatment of abnormal uterine bleeding, amenorrhea, endometriosis and as contraceptive. Drug loaded Chitosan- Na Alginate implants were prepared in the ratios of 50:50, 60:40 and 70:30. As the 60:40 chitosan-sodium alginate ratio showed maximum sustained effect (8 days) it was further tested for sustained release potential with different excipients namely, Stearic Acid, Glyceryl Monostearte, Cety Alcohol and Dextrose. Implants with Glyceryl Monostearte sustained the release of drug the most (23 days). Effects of excipients were also observed on drug loading efficiency. Morphology...
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Posted by admin on Apr 30, 2016 in |
This study evaluates the effect of ciprofloxacin (CIP) on the antimalarial activity of artesunate (AS), artemether-lumefantrine (AL) and artesunate-amodiaquine using animal model. Some 120 albino mice infected with chloroquine sensitive Plasmodium berghei NK65 strain were used. The study was carried out in three phases. Phase 1 consisted of eleven groups treated with different doses of either AS, AL, ASAQ or CIP alone. Phase 2 consisted of nine groups treated with 7 mg/kg of CIP (CIP1) combined with different doses of AS, AL, ASAQ. Phase 3 consist of ten groups treated with 14 mg/kg of CIP (CIP2) with different doses of AS, AL, ASAQ. Thin blood films were used to assess parasitemia level daily after administration of drugs for 72 h. Results were analyzed using student t-test and analysis of variance (ANOVA). CIP alone showed antimalarial activity with 63 % parasitemia reduction. Antimalarial activities of AS and AL were significantly enhanced by both 7 and 14 mg/kg of CIP. The antimalarial effect of ASAQ was enhanced but not statistically significant....
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Posted by admin on Apr 30, 2016 in |
This experimental work aimed at formulating and systemically characterizing mucoadhesive amoxicillin microspheres for the eradication of H.pylori and its associated diseases. The microsphere batches were prepared by emulsion-solvent evaporation technique with the use of carbopol-934P as mucoadhesive polymer and ethyl cellulose as carrier polymer. Initially 27 formulations were prepared using 33 factorial design using Design Expert software. Results of preliminary trials showed that amount of drug-to-polymers ratio, emulsifying agent and stirring speed influenced the characteristics of microspheres. Prepared microspheres were discrete, spherical, free flowing with good percentage of drug entrapment. An in-vitro mucoadhesive test showed that amoxicillin microspheres adhered more strongly to the gastric mucous layer and could retain in the gastrointestinal tract for an extended period of time. A 33factorial design was employed to study the effect of independent variables, drug-to-polymer-to-polymer ratio (amoxicillin-ethyl cellulose-carbopol-934P) (X1), Emulsifying agent (X2) and stirring speed (X3) on dependent variables, drug entrapment (Y1), particle size (Y2). The best batch exhibited a high drug entrapment efficiency of 66% and In vitro mucoadhesion after 1...
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Posted by admin on Apr 30, 2016 in |
A simple, rapid and precise stability-indicating ultra high performance liquid chromatography (UHPLC) has been developed and validated for the estimation of telmisartan in drug substance and pharmaceutical dosage form. The chromatographic separation was achieved with a Poroshell 120EC-C18 column (4.6 x 50mm, 2.7µm) by using mobile phase acetonitrile: 50mM ammonium acetate buffer in the ratio of (45: 55 v/v), pH adjusted to 4.5 with acetic acid. The instrumental settings were flow rate of 0.5ml min-1, column temperature at 25 0C and detector wavelength of 290 nm using a photodiode array detector. Telmisartan was exposed to thermolytic, photolytic, acid, base, hydrolytic and oxidative stress conditions and the stressed samples were analyzed by the proposed method. The developed method shows excellent linearity over a range of 100-300 µg ml-1 for telmisartan. The recovery of telmisartan was above 96%. The proposed method was found to be suitable and accurate for quantitative determination and stability study of telmisartan pharmaceutical...
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Posted by admin on Apr 30, 2016 in |
The current investigation was carried out to develop and validate a fairly simple, accurate, precise, reproducible and robust RP-HPLC method for the estimation of Ticagrelor Hydrochloride. The separation was achieved using Agilent Infinity 1220, Infinity Fast-LC (Pressure limit up to 600 bars) with auto sampler and PDA detector. The Chromatographic analysis was performed on ZORBAX Eclipse Plus 300SB C18 (250 x 4.6mm, 5.0 micron, PN 880995-902) column. Mobile phase consist of (A) Acetonitrile: (B) 20mM Potassium dihydrogen ortho phosphate buffer (40:60 v/v) at a flow rate of 1.0 ml/min. The method showed linear in the mentioned concentrations having line equation y = 22.848x + 1.3214 with correlation coefficient R2 of 0.9995. The recovery values for Ticagrelor ranged from 99.63% to 100.34%. The % RSD was 0.49% and 0.54%, respectively for intraday and interday precision. The limit of detection and limit of quantification were 0.05μg/mL and 0.20μg/mL respectively. Newly developed method was statistically validated for accuracy, precision, linearity and solution stability; hence it is directly applicable for the estimation of...
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