Posted by admin on Feb 29, 2024 in |
Aspartame is the most common artificial sweetener which is known to induce oxidative stress. Our goal was to determine whether Boeravinone B and Chebulinic acid protect HepG2 cells against Asp-induced oxidative stress in HepG2 on a multifarious level. In this investigation we have addressed ameliorative role of BB and CA in due course of Asp exposure by evaluating biochemical and molecular parameters. We found that Aspartame induced oxidative stress in HepG2 cells through elevation of ROS and RNS which was further attenuated by BB and CA. Associated levels of SOD, CAT, GPx, Protein carbonyls, Lipid peroxides and Caspase 3/7 in Asp treated cells were found to be risen but BB and CA supplementation restored these back to normal. It was revealed from fluorescence microscopy as well as flow cytometric analysis that BB and CA reversed the proportion of apoptotic, necrotic as well as autophagic cells which was found to be triggered during Asp exposure. mRNA expression of marker genes associated with Apoptosis, Necrosis and Autophagy was observed to be...
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Posted by admin on Feb 29, 2024 in |
Changes in drug prescribing-pattern in different groups of population and age-related pathophysiological changes may result in altered response of drugs and need proper monitoring to avoid adverse consequences. Polypharmacy is very common in elderly which further increases risk for unwanted effects. Moreover, physicians should be more familiar with the common problems and diseases occurring amongst elderly population and the drugs safe for this age group. Present study has been done to know the pattern of diseases occurring amongst elderly and commonly prescribed drugs for this population. Study-design was cross-sectional and it included private medical practitioners at Lucknow. Patients above 60 years of age attending these physicians were interviewed personally using a questionnaire. Thus, this study describes pattern of medication consumption by elderly patients among community dwelling in urban and rural areas of Lucknow. Out of 112 subjects interviewed, 51.5% elderly were prescribed three or more medications. Overall consumption varied from 1 to 10 different medications per day. Out of all medications prescribed; antihypertensives ranked first followed by analgesics, sedative-hypnotics,...
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Posted by admin on Feb 29, 2024 in |
The advancement of drug therapy has undoubtedly elevated patient care standards. However, it has also given rise to an increase in Drug-Related Problems (DRPs), which can significantly disrupt health outcomes. In an 18-month prospective observational study involving 2,000 patients, we meticulously assessed DRPs using the Hepler and Strand classification system. These DRPs encompassed a range of issues, including adverse drug reactions (ADRs), untreated medical indications, non-compliance with prescribed regimens, improper drug selection, and more. Our data collection adhered to a well-validated and pre-tested format. Among 2,000 patients, 106 (5.3%) experienced 117 DRPs, averaging 1.1 per patient. Adverse drug reactions (ADRs) accounted for 47.01% of cases, non-compliance 35.89%, and drug use without indication 11.32%. ADR incidence was 2.75%, primarily affecting the Gastrointestinal (GIT) system. Antibiotics were frequently associated with ADRs in various drug classes. Vigilant monitoring, robust reporting mechanisms, and patient education are pivotal strategies in the prevention of drug-related issues. Pharmacovigilance, the systematic monitoring and evaluation of drug safety and efficacy, plays a fundamental role in ensuring the delivery...
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Posted by admin on Feb 29, 2024 in |
The creation of reliable bioanalytical LC-MS (liquid chromatography-mass spectroscopy) methods is crucial for the discovery, development, and eventual marketing approval of new drugs. A brand-new medication called siponimod was developed to treat Multiple Sclerosis (MS). This medication is a sphingosine-1-phosphate (S1P) receptor modulator and is hypothesised to contribute to the reduction of MS-related inflammation of the central nervous system. Since the last several decades, the usage of ant sclerosis agents has grown dramatically, however there is currently no bio analytical approach available for quantifying siponimod in biological matrix investigations employing LC-MS/MS. To create a novel, quick, and accurate LC-MS/MS approach for simultaneously measuring siponimod in rat plasma utilising the liquid-liquid extraction method (LLE) and deuterated siponimod (SId6). Method: Chromatographic separation was performed in isocratic mode using acetonitrile and OPA buffer at a flow rate of 1 ml/min on a reverse phase Phenyl C18 (150 mm x 4.6 mm, 3.5 m) column. Utilising a positive-mode electrospray ion interface and multiple reaction monitoring (MRM) procedures, quantification was accomplished. Over the concentration...
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Posted by admin on Feb 29, 2024 in |
Currently, the arise of drug resistance and undesirable off-target effects of anti-cancer agents are major challenges for cancer treatment, which energizes medicinal chemists to develop more anti-cancer agents with high efficiency and low toxicity continuously. sulphonamide derivatives are a class of promising compounds with diverse biological activities including anti-cancer, and parts of them have been marketed for cancer therapy. In this review, we summed up the synthesis of sulphonamide derivates and their anticancer activities. Today Cancer remains to be one of the deadliest diseases in the world. Due to the potential anticancer activity of the campholinicaldehyde and sulfonamide moieties, five novel hybrid compounds containing both structures have been designed and synthesized in 3 steps. The synthesized compounds were characterized on the basis of IR, 1H NMR, 13C NMR spectral data, and elemental analysis and also, they were screened for in-vitro anticancer activity on human breast cancer cell line MCF-7 and HELA by the MTT assay method. Among them,4-fluoro-N-(4-(4-oxo-2- ((2,2,3-trimethylcyclopent-3-en-1-yl) methyl) thiazolidine-yl)phenyl) benzene sulfonamide (Fig. 5b) showed the most potent...
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