Posted by admin on Jun 30, 2016 in |
Lactobacillus plantarum culture supernatants (LAPS) have antimicrobial, pro‐healing and anesthetic properties, so our medical team applied whole cultures and LAPS in chronic wounds with encouraging results. Metabolites responsible for LAPS properties and its action mechanism were previously determined. Objective: to design methods and culture media to increase the production of LAPS metabolites and optimize it´s therapeutic effectiveness. Modifications in MRS broth composition were made and L. plantarum was cultivated in the resulting different media with different physicochemical conditions to obtain modified supernatants (LAPSm). Antimicrobial and anti-biofilm activity (crystal violet) of LAPSm on strains typically isolated from chronic wounds (Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis and Serratiamarcescens), was tested. Concentration of barbiturates in LAPSm was quantified by GCMS and its relative anesthetic potency in healthy volunteers was evaluated. DNAase and auto inducers type 2biological activity in LAPSm were quantified. In addition, a safety assessment of LAPSm application on healthy skin of 200 volunteers was performed. LAPSm obtained from media with high concentrations of yeast extract possessed the greatest anesthetic power....
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Posted by admin on Jun 30, 2016 in |
Myocardial infarction develops due to ischemia induced oxidative stress. Averrhoa Carambola fruit (ACF) is reported to possess strong antioxidant activity which may protect the heart against isoproterenol (ISO) induced myocardial infarction (MI) in rats. To evaluate this activity Averrhoa carambolafruit extract (ACFE) (200mg/kg & 400mg/kg, p.o) was administered to male wistar rats (180-250g) for 21days. At the end of this period, all the rats except rats of normal control group were administered ISO 85mg/kg intraperitonially, for 2 consecutive days to induce MI. After 48 hrs, rats were sacrificed and the level of cardiac biomarker enzymes like cardiac troponin-T (cTn-T), creatinine kinase (CK-MB) and lactatedehydrogenase1(LDH1) in serum were estimated. Also antioxidant parameters likeglutathione (GSH), malondialdehyde (MDA), catalase and superoxide dismutase (SOD) were estimated in heart tissue homogenate and histopathology of heart tissue was performed. Our results suggest that pretreatment of animals with ACFE maintain near normal levels of cardiac biomarker enzymes and antioxidant enzymes in both treatment groups (ACFE 200mg/kg, 400mg/kg) when compared to ISO treated group. Histopathological findings of...
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Posted by admin on Jun 30, 2016 in |
Azetidin-2-one analogues are reported to exhibit various pharmacological activities like cholesterol absorption inhibitory activity, human tryptase, thrombin and chymase inhibitory activity, vasopressin V1a antagonist activity, antidiabetic, anti-inflammatory, antiparkinsonian and anti-HIV activity in addition to antimicrobial.1-6 In the present study, Isoniazid (INH), the established antitubercular drug was selected as the lead for the design and development of antitubercular agents with minimal toxic effects. A novel series of amino azetidinones were designed from corresponding azetidin-2-ones using various in silico methods. Docking studies were performed at Mtb enoyl acp reductase (4DRE) and the derivatives exhibited best docking scores were prepared from corresponding azetidin-2-ones by treating with various molecules containing amino groups in the presence of TEA. Azetidin-2-ones in turn were obtained from a series of INH Schiff bases by reaction with chloro acetyl chloride. Structures of the newly synthesized compounds were assigned on the basis of elemental analysis, IR, 1H NMR, 13CNMR and mass spectral studies. The newly synthesized compounds were screened for their in vitro antitubercular activity using Alamar blue assay...
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Posted by admin on Jun 30, 2016 in |
Forensic science is growing rapidly in the world today. During the past ten years, medico-legal investigations highly expanded to include all areas of forensic science. The present aim of project investigated 29 samples of blood and 29 samples of saliva swabs that were collected from human volunteers. The saliva samples were collected by buccal swab but the blood samples were collected by Bode Secur Swab S.I.T. Collector. The experiments were done at four different temperatures (55°C, 37°C, 24°C and 4°C) for 28 days. The results showed that, DNA quantity that was investigated at a temperature of 4°C and 24°C in both blood and saliva samples was more or less remained the same during the whole period of the study, comparing values for day one with all other days including day 28. DNA quantification of human blood following extraction at 37°C was 46.14 ng/μl ± 0.22 at day one then starts to decrease until it reached 36.05 ng/μl ± 0.07 at day 28. In contrast, the result obtained from real-time...
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Posted by admin on Jun 30, 2016 in |
Background: In present study, we aim to assess the anticandidal activity of crude extracts and screening of bioactive compounds and their identification by NMR, IR and GC-MS. Results: A total of 192 isolates were isolated from patients having oral and vaginal candidiasis and a total of 134 isolates showed positive results with Gram staining. The selected isolates APKU-2, APKU-3 and APKU-4 were identified as C.albicans, C.lusitaniae and C.krusei by studying its morphological, biochemical, and molecular characteristics. Chemical characterization of the extracted compounds was done by H1NMR, Fourier transform infrared spectroscopy (FTIR), Gas chromatography–mass spectrometry (GC-MS) analysis to determine the structure, melting point, and molecular mass of the purified compounds. Conclusions: The crude extracts and purified compounds from the sandalwood holds potential as medications for various antifungal therapeutics. However, further research on their potential incorporation into different preparations, safety and cost- effectiveness remains to be...
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