Posted by admin on Mar 31, 2016 in |
Four crude extracts viz, methanol extract of fruit and n-hexane, dichloromethane, methanol extracts of the leaf of R. tetraphylla were investigated for in vitro antioxidant activity at different concentrations (5, 50 and 100 μg). Antioxidant ability is expressed as equivalents of ascorbic acid and was calculated using standard graph. The results indicated that the leaf n-hexane and methanol extracts was found to be significantly active at 5μg when compared with BHA, and at 50 μg concentration the methanol leaf extract found be very high. The fruit methanol extract was found to be active and the activity increased with increase in dose, but not as that of BHA. The experimental results show that the activity exhibited by the solvent extracts is dose...
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Posted by admin on Mar 31, 2016 in |
The present study relates to the stable fixed dose combination comprising of Aceclofenac as a glycolic acid ester of Diclofenac is having analgesic and anti-inflammatory action and Thiocolchicoside, as a glycosulfurated analogue of colchicine which is well known centrally acting muscle relaxant in unit dosage form and to the preparation method thereof. Aceclofenac is very susceptible to decomposition under hydrolytic, oxidative stress and exposure to light. The major degradation product identified was diclofenac, which was detected as an impurity in the formulation. Many of the marketed formulation of Aceclofenac tablets having problem of high content of diclofenac impurity. Diclofenac having more gastrointestinal adverse effects such as gastritis, gastrointestinal haemorrhage, haemorrhagic diarrhoea, gastrointestinal ulcer than that of Aceclofenac. It was challenge to develop Aceclofenac tablet formulation having diclofenac impurity within compendial limit. The compatibilty of Aceclofenac with various tableting excipients was investigated physically and by means of Differential Scanning Calorimetry (DSC). Aceclofenac alone and with other excipients in 1:1 binary mixtures were investigated before and after accelerated storage. An interaction...
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Posted by admin on Mar 31, 2016 in |
Coleus forskohlii is an Indian medicinal plant it treat various diseases of cardiovascular, respiratory, gastrointestinal, and central nervous systems. The purpose of this study was to investigate the anticancer activity of an ethanol extract of Coleus forskohlii against gastric cancer in AGS cancer cell lines. Gastric carcinoma cells were tested in vitro for cytotoxicity, Ao/EtBr staining for apoptotic cells, apoptotic DNA fragmentation in response to Coleus forskohlii extract. The protein expression of Bax, Bcl2 and Caspase-3 were analyzed through Western blotting. Treatment with Coleus forskohlii extract inhibited AGS cell proliferation dose and time dependently. Cells exposed to Coleus forskohlii extract showed typical hallmarks of apoptotic cell death. In conclusion, Coleus forskohlii ethanol extract has cytotoxic and apoptotic effects on Gastric carcinoma cells. Its mechanism of action might be associated with the apoptosis induction through up-regulation of the protein expressions of caspase-3 down-regulation of the protein expression of Bax and...
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Posted by admin on Mar 31, 2016 in |
Stability indicating RP-HPLC gradient method developed for simultaneous determination of impurities and degradation products from Emtricitabine and Tenofovir Disoproxil Fumarate in pharmaceutical tablet dosage form. The chromatographic separation was achieved by using column ACE C18 (250 x 4.6, 5μ). The mobile phase-A consists of 0.01M potassium dihydrogen phosphate buffer with pH 4.0 adjusted using diluted ortho-phosphoric acid and mobile phase-B as methanol. The flow rate was 1mL min-1 throughout the gradient program with detection wavelength of 270 nm for both components with its related impurities. The column temperature was 30°C and injection volume of 20µl. A well separation of degradation products from main peak and respective known impurities was found with resolution between adjacent peaks greater than 2.0. The method found linear from LOQ to 0.4% level for all impurities with respect to concentration for Emtricitabine(0.7 mg/mL) and Tenofovir Disoproxil Fumarate (1.06 mg/mL) The developed method was validated for specificity, linearity, precision, LOD, LOQ, accuracy and robustness as per ICH guideline Q2A (R1). The results were indicating that the...
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Posted by admin on Mar 31, 2016 in |
A series of six new mixed metal complexes of general formulation [MM12(L)n]xH2O, where M= Mn, Co, Ni, Cu, Zn, Cd,M1=Fe, L= Tartrate ligand, x=1,5,6 and n = 3, are synthesized using tartrate as a bidented ligand. The newly synthesized complexes were characterized using various analytical techniques such as IR, XRD, TGA, Elemental analysis, magnetic susceptibility study etc. IR spectral data suggested that the bidentate ligand has coordination to both the metal ions through oxygen as donor atoms. The thermal behavior (TGA) of the complexes was studied; suggest the decomposition patterns were in good agreement with recommended formulae of the complexes. XRD technique revealed the presence of crystalline nature of all the studied complexes. The antibacterial activity of the metal complexes along with ligand was assayed against three bacterial pathogens viz. Bacillus Megatherium, Staphylococcus aureus, E-Coli, Pseudomonas and Proteus Vulgaris by well diffusion method. The bioassays of all the metal complexes showed a greater inhibitory effect in the form of broad activity spectrum than the individual ligand, which indicate an...
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