Posted by admin on May 31, 2016 in |
This study aimed to develop and optimize a multi-particulate formulation for colon targeted delivery of rifaximin by application of a functional coat of Eudragit L100 and Eudragit S100 mixture on the drug layered pellets. The rifaximin-excipients compatibility was confirmed with FTIR studies. Functional coat completely restricted drug release in pH 1.2 dissolution medium. Statistical optimization of the formulation variables, i.e., ratio of Eudragit L100 and S100, and % of coating, in respect to drug release at 2nd and 8th h in pH 6.8 dissolution medium was conducted with a central composite design and response surface methodology. Compared to % of coating, the impact of polymer ratio was more pronounced for modulating the drug release. Comparatively, higher influence of % coating was observed after 2 h of dissolution. As per graphical optimization, the minimum drug release at 2nd h and maximum drug release at 8th h could be achieved in formulation with 3.3 polymer ratio and 15.4 % coating where more than 80 % of drug should be delivered in...
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Posted by admin on May 31, 2016 in |
Peptide deformylase (PDF) is a mononuclear metal ion protein that is responsible for removing the N-formyl group of nascent proteins found in bacteria and chloroplasts in order for them to become mature proteins. From literature it was found that heterocyclic derivatives containing hydroxamic acid and hydroxyl amine exhibit good antibacterial and PDF inhibitory activity. Heterocycles bearing nitrogen, sulphur, oxygen and thiazole moieties constitute the core structure of a number of biologically interesting compounds. Recently 1,3,4 oxadiazole and 1,3,4 –triazole derivatives received significant attention owing to their diverse range of biological properties particularly being antifungal, antitubercular, antibacterial, antiviral, anticancer and antioxidant. Ethyl-cyano- acetate when treated with sodium hydrate and THF give acetate derivatives, which on further treatment with hydrazine hydrate and various reagent afforded heterocyclic hydroxylamine...
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Posted by admin on May 31, 2016 in |
A study on biological monitoring of simultaneous exposure to benzene and alcohol was made in petrol pump operators of Meerut city (India). In addition to alcohol, other confounding factors viz. cigarette smoke, food habit, age and work experience were also considered. Thirty two, 20-40 years old male workers were registered for this study. Respective urine samples collected after the workshift were analyzed for specific gravity, creatinine and phenol. Phenol concentration corrected to creatinine was found to be low (22.22 mg/g creatinine) in alcoholic pump operators in comparison to non-alcoholic operators (24.09 mg/g creatinine). It has been suggested that acute ethanol ingestion inhibits benzene oxidation in the liver with an ethanol inducible form of CYP450. Although urinary phenol concentration in the registered Indian petrol pump operators is less than BEI as suggested by ACGIH (50mg/g creatinine), simultaneous exposure to alcohol and benzene may potentiate the hepatotoxicity, cytotoxicity of benzene amongst petrol pump...
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Posted by admin on May 31, 2016 in |
A method using derivative UV spectrophotometry was developed and validated for the determination of methylxanthines and tannins in guarana bulk, using caffeine and catechin as markers. The spectra were obtained using first order derivatization, Δλ=20.000, and scale factor of 10. The quantification of actives was performed using wavelengths of 244 and 277 nm for catechin and caffeine, respectively. The parameters of specificity, linearity, precision, accuracy, robustness, limits of detection (LOD) and quantification (LOQ) were established to validate the method. The method was shown to be specific for quantification of methylxanthines and tannins, and linear over the concentration range 5–35 µg mL-1, with a correlation coefficient of r=1(caffeine) and r=0.9993 (catechin). The results indicated good intra-day and inter-day precision with RSDs less than 2%. The method was accurate, exhibiting recoveries between 98 and 102%. The LODs were 0.184 µg mL-1 (caffeine) and 0.368 µg mL-1 (catechin), and the LOQs were 0.552 µg mL-1 (caffeine) and 1.104 µg mL-1 (catechin). Methylxanthines and tannins were quantified, and contents of 10 µg mL-1...
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Posted by admin on May 31, 2016 in |
The availability of several brands of Simvastatin tablets in Libyan pharmacies today places health practitioners in a problem of generic substitution. The aim of the present study was the evaluation and comparison between four different Simvastatin brands, which are commercially available in the Libyan market produced by various pharmaceutical companies with different trade names. The physicochemical equivalence of four brands of Simvastatin tablets was investigated through the evaluation of both official and non-official standards according to the USP pharmacopoeia including uniformity of weight, thickness, hardness, disintegration time, drug content as well as dissolution rate. Acceptable external features as well as uniformity in diameter and thickness revealed for all the tablets. The entire brands complied with the official specifications for uniformity of weight where no tablet showed a deviation more than ±7.5%. Brand B had the highest crushing strength and highest disintegration time compared to the other brands. All the brands had values within the range specified for assay in the BP. The dissolution profiles showed that none of the...
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