Posted by admin on May 31, 2016 in |
An experimental study was carried out after getting approval from institustional animal ethical committee to study the role of oral iron chelator, deferasirox in prevention of myocardial ischemic reperfusion injury in albino rabbits. Albino Rabbits of either sex were divided into two groups control (n=5) and test group (n=5), receiving deferasirox. Experiments were done using isolated heart perfusion apparatus (Langendorff apparatus). The experiment was divided into three phases, perfusion (15 min) followed by ischemia (10 min) followed by reperfusion (15 min). LDH level of perfusate and coronary flow were taken as biochemical and physiological marker of myocardial reperfusion injury respectively and were measured at 5, 10 and 15 min of post ischemic period. Control and test groups were compared. The result shows that in the group receiving oral iron chelator, deferasirox, the decrease in post ischemic LDH level was significant (p< 0.01) at 5, 10 and 15 min while there was significant increase in post ischemic coronary flow at 5 min (p< 0.01) as compared with control. Thus oral...
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Posted by admin on May 31, 2016 in |
Amino acid chelated complexes were synthesized and characterized to determine their purity. The purity of the complexes was found to be ranging from 42-75%. The HPLC method for determination of free amino acid content in the complexes was developed and validated and found to be linear, precise and accurate within concentration range of 0.157 mg/mL to 20.14 mg/mL. The method was used to estimate the purity of the amino acid complexes. The changes in the carboxylate and amine group stretches in FT-IR indicated the association of metal ion with the amino acid. The initial studies described above demonstrated that the chelated complexes can produce sufficient osmotic pressure to be used as ergogenic...
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Posted by admin on May 31, 2016 in |
Introduction: Epidural anesthesia is the most commonly used technique for providing not only peri-operative surgical anesthesia but post-operative analgesia in lower abdominal and limb surgeries. Our study aimed to assess the comparison between ropivacaine(Group A) and combination of ropivacaine with fentanyl (Group B) in terms of their analgesic effect, hemodynamic stability, side effects and requirement of rescue drug in post operative patient of total knee replacement surgery. Materials and methods: After surgery, Pulse rate, systolic and diastolic blood pressure, Sp02, visual analogue score and requirement of other analgesics was studied at every 30 minutes for 2 hrs, then hourly up to 12 hrs and every 2 hourly thereafter up to 24 hrs in group A and Group B. Patients were also observed for side effects. Statistically significant when the p <0.05 and highly significant if p<0.001. Results: In our study, there was no significant difference amongst the groups in respect to age, sex distribution, height and ASA physical status. There was no statistically significant difference in pulse rate, blood...
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Posted by admin on May 31, 2016 in |
Medicinal Plants are endowed with phytochemicals that are vital to counter various metabolic disorders like Oxidative damage in cells causing various degenerative diseases. Hence, the present study deals with the assessment of antioxidant activity and phytochemical screening of the aqueous extract of Jasminum officinale leaves. Antioxidant potential was evaluated in vitro using free radical scavenging assays for DPPH, NO, superoxide and ABTS radicals in addition to Reducing Power assessment. The total phenolic, flavonoid and flavonol contents of the extract were also assessed using standard procedures. The phytochemical analysis of the extract revealed the presence of tannins, flavonoids, terpenoids, alkaloids, steroids and its glycosides in addition to saponins and coumarins. A positive correlation between the inhibition of free radicals and concentration of the extract suggests significant antioxidant potential of the extract. The IC50 values for DPPH, NO, superoxide and ABTS radicals were 41.16, 30.29, 20.19, and 29.48 µg/ml respectively as compared to the standard, ascorbic acid, having 42.79, 36.74, 38.22, and 45.57 µg/ml, for the same radicals. It is interesting...
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Posted by admin on May 31, 2016 in |
Objective: To develop and validate a novel and easy first order derivative spectroscopic method for the simultaneous determination of multicomponent dosage form which contains Methylcobalmine, Alpha lipoic acid and Pregabaline. Method: Measurement was achieved by selecting different Zero crossing points i.e ZCP for Methylcobalamine and Alpha lipoic acid at 436.24 nm, ZCP for Alpha lipoic acid and Pregabalin at 338.0 nm and ZCP for Pregabalin and Methylcobalmin at 307.03 nm. The method was validated according to ICH guideline. The proposed method was applied for quantification of all three drugs in the marketed formulations. Results: The method was found linear in the range of 100-140µg/ml for Pregabalin,1-1.4µg/ml for Methylcobalmine and 130-170µg/ml for Alpha lipoic acid respectively. The co-efficient co-relation was found to be 99.5 % for Pregabalin, 99.56 for Methylcobalmine and 99.61 for Alpha lipoic acid. The accuracy and precision were within acceptable limits. Conclusion: The method is simple, accurate, precise, rapid and cheap. The proposed method can be used for analysis of multicomponent marketed...
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