Posted by admin on Feb 29, 2024 in |
The incidence of colorectal cancer in India ranks fourth in men and third in women. Carcinogenesis is a process that involves a combination of mutations in oncogenes, tumour suppressor genes or epigenetic changes in DNA such as methylation. Our study aim to assess the clinicopathogical significance of serum Prolactin, CA19-9 and C – reactive protein in colorectal cancer. Population based case control study, which includes 120 participants and 120 healthy volunteers during the time period of one year. In our study we found, CA19-9 tumor markers reflected more specificity as compared to sensitivity and efficiency. Prolactin were significantly higher when compared with control. Further prolactin levels were significantly elevated in different stages of CRC malignancy as compared to controls. Our values demonstrated the progressive increase corresponding to clinical staging, tumor size and notable in relation to nodal status of colorectal squamous cell carcinomas. Our results may provide new approaches in the diagnosis, prognosis, and monitoring the risk factors and combines both gross as well as microscopic information to determine...
Read More
Posted by admin on Feb 29, 2024 in |
Tartrazine is a lemon-yellow synthetic food colourant belonging to azo group. The Acceptable Daily Intake (ADI) of tartrazine is 7.5mg/kg However, it is possible that more than permissible amount of Tartrazine is used to make food items more attractive. This may pose threat to consumer’s health. Hence the overall aim of the study was to detect and quantitate the presence of tartrazine in commercially available as well as locally made and sold fruit juices and jams. Fresh fruit juices (Branded and Non-Branded) and jams purchased from shops and street vendors in Belagavi city of Karnataka, India were subjected to High Performance Thin Layer Chromatography (HPTLC). Silica gel aluminum plate 60F254 was used as the stationary phase and Iso-propanol: Ammonia (6:4) v/v was used as mobile phase. HPTLC has many advantages over the other analytical methods applied for tartrazine estimation. The method was validated using analytical parameters like linearity, range, precision, specificity, LOD, LOQ and robustness. Out of the 10 branded fruit juices analysed tartrazine was present in 9 samples...
Read More
Posted by admin on Feb 29, 2024 in |
A simple, precise and sensitive High Performance Liquid Chromatographic method has been developed for the quantitative estimation of Lafutidinein bulk and tablet dosage form. The method was performed on Silicagel 60 F254 aluminum plate. A Camag HPTLC system comprising of Camag Linomat IV semiautomatic sample applicator, Camag TLC Scanner 3, Camag twin-trough chamber (10×10 cm) and Camag CATS 4 software, As mobile phase Ethyl acetate: Toluene: Methanol: Ammonia (6:2.5:1.5:0.1%v/v) was utilized to develop the Chromatographic method. Lafutidinegave separation at Rf value of 0.45±0.03 and scanned at 273 nm. The calibration curve response was observed between 400-1400ng/spot. The linear regression data showed good linear relationship with R2 value of 0.99895. The method was validated in terms of repeatability, precision, accuracy, and specificity according to ICH guidelines. The LOD and LOQ were found to be 19ng/Spot and 59 ng/spot respectively. The developed method was successfully used for assay of Lafutidine in tablet...
Read More
Posted by admin on Feb 29, 2024 in |
In view of rising concern of problem associated with synthetic drugs against inflammation, current research has been focused towards safer, effective and natural compounds. Hence, we aimed at assessing the anti-inflammatory effect of four plants from Solanum species (S. seaforthianum, S. diphyllum, S. sisymbriifollium and S. anguivi) through albumin denaturation assay, trypsin inhibition and RBC membrane stabilization assays. The data showed that the methanol extracts of these plants had concentration dependent protection. S. anguivi extract showed maximum inhibition of albumin denaturation (26.15 ± 0.08%), whereas S. sisymbriifollium extract displayed maximum (16.51 ± 0.002%) trypsin inhibition. With respect to heat induced RBC lysis, S. anguivi extract showed highest inhibition (26.16 ± 0.08%), whereas S. seaforthianum extract displayed maximum inhibition (36.49 ± 0.002%) of RBC lysis induced by hypotonicity. From the study, it can be inferred that anti-inflammatory activity in the tested Solanum species extract varied considerably and its activity can be attributed to its richness in phytochemical...
Read More
Posted by admin on Feb 29, 2024 in |
The Cu-Atropine complex may have potential applications in medicinal chemistry, as atropine is used in the treatment of certain medical conditions such as bradycardia and as a pre-anesthetic to decrease salivation. Additionally, the complexation of atropine with copper could potentially enhance its therapeutic effects or alter its pharmacokinetic properties. In the present study, the drug has been modified by complex formation with absolute methanol and concentrated sulphuric acid following standard procedures to increase its potency. IR spectra of Cu- atropine complex has taken. The analytical data indicates that a complex form of the drug, Cu-Atropine, is formed in a 1:1 ratio. To study the interaction between drugs and metal complexes, differential pulse polarography (DPP) has been used. These techniques are simple and cost-effective, making them suitable for qualitative and quantitative determination of active drug compounds. In a pharmacological study, modified atropine showed more potency compared to its parent...
Read More
