Posted by admin on Mar 31, 2016 in |
Aim: Evaluation of the antimicrobial, analgesic and antioxidant activity of ethanolic extract of the leaves of Averrhoa Carambola. Materials and methods: To test the antimicrobial activity the organisms used were S. aureus, Klebsiella sp, E. coli and P. aeruginosa, C. albicans. Zone of inhibitions produced by sensitive organisms were demarcated by a circular area of clearing around plant extract impregnated discs and were compared with zone of inhibitions of positive controls. Analgesic activity was tested by two methods: acetic acid induced writhing test and Eddy’s hot plate mediated pain reaction. The animals were divided into 5 groups: Group I (Normal control), Group II (Standard drug), Group III, IV, V (Etahnolic extract of A.carrambola in the doses of 100, 200 and 400mg/kg respectively). To test the antioxidant activity, the mice were divided into 6 groups containing. Paracetamol in the dose of 250mg/kg p.o was administered to all the groups except the first group, which was taken as the normal control, for 10 days to induce oxidative stress. Silymarin in the...
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Posted by admin on Mar 31, 2016 in |
Calotropis gigantea L. flowers were widely used as a natural herbal medication. The objective of the present study was to screen the phytoconstituents and to evaluate the antibacterial and anti-cancer properties of Calotropis gigantea flower extract. The UV-Vis and FTIR spectroscopic techniques were employed to screen the phytochemicals. The antibacterial property of the extract was evaluated through serial broth dilution technique. Cell viability was performed using MTT assay on HeLa cell lines. The presence of Phenolic compounds was confirmed by UV-Vis peak at 271nm. IR spectrum confirms the presence of alcohol/phenols, primary amines, aldehydes, alkanes, alkenes, sulfoxide and halogen compounds. The extract showed antibacterial activity against Enterococcus faecalis (75µg/ml) and Klebsiella pneumonia (2µg/ml). The aqueous ethanolic extract of Calotropis gigantea flower exhibited less activity on HeLa cancer cell viability even at higher concentrations (1000µg/ml). The application of spectroscopic techniques promises to be of a great value in screening phytoconstituents of the extract because of their simplicity and cost-effectiveness. The extract is capable of inhibiting the bacterial growth and may...
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Posted by admin on Mar 31, 2016 in |
The aim of the present study is to evaluate the gum of Curcuma amada (mango ginger) as a tablet binder employing paracetamol as a model drug. Natural gums are economic, easily available and found useful as tablet binder. The physicochemical properties of a gum obtained from the rhizomes of Curcuma amada were characterized by Fourier transmittance infra red (FTIR) and Differential scanning colorimetry (DSC). Amada gum has a glass transition (Tg) temperature and melting peak at 94.3°C. Paracetamol tablets were prepared by wet granulation technique using amada gum as a tablet binder in the concentrations of 1%, 3%, 5%, 7% & 9% w/w and compared with standard binders. The prepared tablets were evaluated for tablet characteristics. The values of granules showed angle of repose good flow property. The hardness of the prepared tablets varies with binder concentration. The prepared tablets showed friability decrease with increases concentration and disintegration time increase with increase the binder concentration. In vitro drug release was also depending on binder concentration. Tablets with 1%and 5%...
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Posted by admin on Mar 31, 2016 in |
The combination of agarwood (Aquilaria crassna) young leave with α-tocopherol (α-TOH) showed a synergistic effect which helps to protect the food from lipid oxidation. The present study was designed to elucidate the acute toxicity of the mixture of A. crassna young leaves crude ethanolic extract (CE) and α-TOH on Balb/c mice. This acute oral toxicity study was carried out based on OECD 423 guidelines by using female Balb/c mice weighing 15-25 g. First group was served as control group which received dimethyl sulfoxide (vehicle) while second, third and fourth group were orally administered with single dose of 2000 mg/kg of CE, α-TOH and a mixture of CE and α-TOH (1:2), respectively. Throughout 14 days of observation, no behavioural changes were seen in all the treated animals. Body weight for each mouse and the relative organ weights for liver, kidney, lung, heart and spleen showed insignificant different (p>0.05) when compared to the control group. These results indicate the safety of the acute exposure of the A. crassna CE, α-TOH and...
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Posted by admin on Mar 31, 2016 in |
An efficient and sensitive RPHPLC method was developed and validated for the estimation of Sitagliptin Phosphate process impurities in drug substances which were identified and characterized by LCMS, FTIR, 1H NMR, C NMR techniques. The method was carried out on a Symmetry shield RP 18 column at 25°C using a 1 % Perchloric acid and acetonitrile in gradient mode of pump. The flow rate is 1.2 ml/min and detection was done at 210nm.The developed RP HPLC method was validated by testing specificity, precision, Forced degradation, detection limit, quantification limit, linearity ,accuracy, robustness and range. The linearity of the method was confirmed over the range of 0.113 to 3.384µg/ml for Sitagliptin Phosphate impurities with correlation coeffients greater than r=0.999.The accuracy of the method was found to be 98.5 to 101.1% and %RSD as found to be less than 2% indicating high degree of accuracy and precision for the proposed method. The effective recovery and lower RSD proves the highness of the proposed RP HPLC method for the routine determination of...
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