Posted by admin on Apr 30, 2016 in |
A simple and accurate Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method has been developed for the estimation of ketoprofen in vitro samples and in vivo samples from bulk drug and from administered transdermal dosage form, using Spherosorb S5 ODS of 10cm X 4.6 mm column, (5μm particle size). Mobile phase for in vitro sample analysis and in vivo plasma samples consists of 0.01 M sodium dihydrogen phosphate (pH adjusted to 6.5 with ortho phosphoric acid), methanol, and acetonitrile, 4:3:3 (v/v) respectively. Isocratic elution technique was followed. The flow rate was 0.5ml/min and the detection was monitored out by UV detector at 265nm. The retention time for ketoprofen was found to be 2.982 in in vitro sample and 3.025 min in in vivo sample. Naproxen was used as internal standard for in vivo sample analysis. The proposed method has permitted the quantification of ketoprofen over linearity in the range of 100-1000...
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Posted by admin on Apr 30, 2016 in |
The lectins are glycoproteins or sugar binding proteins of non-immune origin but are barred from sugar binding antibodies and enzymes. Lectins are isolated and purified from seeds of Glycine max by soxhlet extraction and dialysis. These collected crude lectins were centrifuged till pH is shifted downward to optimal pH for coprecipitation. Filtration of the same carried out on a Buchner funnel with a pad of Hiflo Supercel on whatman paper. Galactose was added as a ligand to the mixture kept at 250C for 10-20 min. It formed matrix coprecipitation which was centrifuged to remove additional particulates. Supernatant was removed and retained the galactose lectin coprecipitate which finally yields lectins, further purified by dialysis. Encapsulation by spray drying using maltodextrin and lactose along with the Eudragit S100 targeted the drug moiety to colon. Purified Lectins have the binding property of carbohydrate moieties on the surface of erythrocytes which agglutinate the erythrocytes, these lectins were evaluated by the agglutination test using ‘A’ positive blood group. These lectins showed anticancer activity against...
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Posted by admin on Apr 30, 2016 in |
Introduction: The Maxillary labial frenum is a fold of the mucous membrane connecting the lip to alveolar process. The maxillary labial frenum is most commonly observed clinically with reference to the midline diastema. Objective: To analyse the most common type of abnormal Maxillary labial frenum in South Indian population and to determine the type of abnormal Maxillary labial frenum commonly associated with midline diastema. Methods: A total of 60 cases with abnormal Maxillary labial frenum from three states were analysed. The age group of the cases was 15 to 25. Cluster sampling technique was used to select the states whereas random sampling technique was used to select the dentists and the samples. Results and conclusion: The most prevalent type of abnormal Maxillary labial frenum was Simple Frenum with Nodule, followed by Persistent tectolabial frenum. There was no statistical correlation between the type of abnormal maxillary frenum and the incidence of midline...
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Posted by admin on Apr 30, 2016 in |
Hyperlipidemia is a common finding in uncontrolled Diabetes Mellitus (DM) and patients with DM generally show alteration in lipid profile, notably in triglycerides value. Research done in the past has pointed out the usefulness of Atherogenic Index of Plasma (AIP) as a useful parameter for the diagnosis of Type 2 DM. HbA1c is well established as gold standard to monitor long term diabetic control. Further elevated lipid profile markers have been linked to a host of diseases like Cardiovascular, liver and kidney. Some studies have shown reasonable association between AIP to plasma glucose and HbA1c, but this study did not find any association between AIP to HbA1c and glucose, but very good association have been observed between Atherogenic ratio to both plasma glucose and HbA1c suggesting that lipid profile test to be made as routine for the diagnostic improvement of all DM patients. The contents of this study will serve as a model for future researchers to explore more studies in this field to define a set of diagnostic...
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Posted by admin on Apr 30, 2016 in |
The aim of this research is to precisely discuss and appraise the success of different approaches to the development to antiplatelet drugs. Platelets play a main function in haemostasis and the development of arterial thrombosis that is the final event complicating cardiovascular diseases and peripheral vascular diseases, and antiplatelet drugs improve survival of patients with these diseases. Antiplatelet drugs are aimed to avert and/or reverse platelets aggregation in arterial thrombosis, most significantly in myocardial infarction (MI), ischaemic stroke, and peripheral artery disease. The current therapeutic strategies aimed at inhibiting platelet aggregation: inhibition of cyclooxygenase, such as aspirin; inhibition of phosphodiesterases III and V and adenosine uptake by red blood cells, such as dipyridamole and cilostazol; inhibition of the platelet adenosine diphosphate (ADP) P2Y12 receptor, such as ticlopidine and clopidogrel; inhibition of glycoprotein IIb/IIIa receptors that prevent fibrinogen binding, such as abciximab; and increasing nitric oxide level, such as triflusal. A range of new drugs are currently in different phases of clinical trials, including reloading of clopidogrel, the improvement of...
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