Posted by admin on Nov 30, 2023 in |
Background: The herbal drugs used in traditional medicine are proven more effective and safe compared to synthetic drugs for managing lifestyle diseases, including liver toxicity. Objective: The present investigation aimed to evaluate the hepatoprotective potentials of ethanol extract of Euphorbia heterophylla leaves against ethanol-induced liver damage in rats. Methodology: The acute oral toxicity study was conducted according to guidelines No 425 prescribed by OECD. The extract was proved safe up to 2000mg/kg. Ethanol-induced hepatotoxicity in rats model was used to assess the protective properties of ethanol extract of Euphorbia heterophylla. The serum, such as (AST), alkaline phosphatase (ALP), total bilirubin, direct bilirubin, albumin, total protein, ions was determined. And other parameters like clotting time, liver weight, liver histopathology, and liver antioxidant enzymes were also examined. Results: In toxic control animals treated with ethanol alone, there were variations in the parameters mentioned above. But in the animals treated with ethanol extract of Euphorbia heterophylla (EEEH) and standard drug silymarin, all the parameters were normal, possibly due to their beneficial property...
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Posted by admin on Nov 30, 2023 in |
Chronic urticaria is a popular skin complaint that is characterized by wheals of various sizes surrounded by erythema and associated with itching and burning as a consequence of Type1 hypersensitivity reaction mediated by IgE antibodies to specific allergen. Most of the population (>20%) have urticaria once somewhere in the life. The conventional management of this condition is associated with long-term effects of anti-allergic drugs and inexorable recurrence. Homoeopathic medicines help to reduce the hypersensitivity and recurrence in a gentle and permanent way. The present study is done to assess the effect of homoeopathic medicine in case of chronic urticaria. Case Summary: The below mentioned case is related to Cholinergic urticaria, one of the types of Physical urticaria in a young male who was given Psorinum as an Individualized prescription. The change in the assessment score done by UAS7 Urticaria Activity Score which was 42 at the baseline and 2 at the end of the treatment. MYMOP2 Measure Yourself Medical Outcome Profile 2 was 4.5 and after treatment was 1....
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Posted by admin on Nov 30, 2023 in |
Lysozyme amyloid formation is studied as model in-vitro system to find out natural or synthetic inhibitors of amyloidosis. Protein aggregation and amyloid formation is the basic reason for many neurodegenerative diseases. Indigenous medicinal plants are great source of therapeutic molecules and drugs. Ethnobotanical studies have indicated many plants have neuroprotective functions. We have studied three indigenous medicinal plant namely Bacopa monnieri (BM), Trigonella foenum-graecum (TFG) and Tridax procumbens (TP) showing various pharmacological properties as inhibitors of lysozyme aggregation. We isolated leaf proteins and did the protein aggregation studies. Anti-aggregation properties of leaf extract were carried out by following assays – DTT induced lysozyme aggregation assay, Congo red binding Assay, Thiaflavin binding assay and protein stability assay by Tryptophan Fluorescence. BM leaf protein extract showed maximum inhibition followed by Methi (TFG) and then...
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Posted by admin on Nov 30, 2023 in |
A simple, precise, accurate, and rapid high performance thin layer chromatographic method has been developed and completely validated for the estimation of hydroxychavicol isolated from piper betel leaves powder. The isolation of hydroxychavicol was carried out using flash chromatography. Validation was carried out with pre-coated silica gel aluminum plate G 60 F254 (10 x 10 cm) as the stationary phase, mobile phase consisting of Butanol: Ethyl Acetate in the composition of 7:3 (v/v) and scanned in Absorbance Reflectance mode at 280 nm using Camag TLC scanner 3 with WinCAT software. The Rf value of 0.64 was obtained for isolated hydroxychavicol. The proposed method was validated over a linearity range of 200–800 ng/spot, and its percentage recovery was calculated along with intraday and interday precision. The limit of detection and the limit of quantification were found to be 13.890 ng/spot and 40.457 ng/spot, respectively. The suggested method can be successfully used to detect and quantify hydroxychavicol in herbal...
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Posted by admin on Oct 31, 2023 in |
A sensitive, rapid, precise and accurate RP-HPLC method was developed for simultaneous estimation of Pregabalin and Duloxetine in synthetic mixture. To introduce a chromophoric group in Pregabalin structure, derivatization was carried out through benzoylation reaction. RP- HPLC method is carried out using Hypersil BDS column C18 (250 cm × 4.6 cm, 5 μm). Mobile phase used as combination of Methanol: Water (75:25 v/v) and detection was carried out at 235 nm & 224 nm respectively for Duloxetine and Pregabalin. Retention time of Duloxetine and Pregabalin were found to be 2.15 min and 5.23 min, respectively. The developed method was validated in pursuance of ICH Q2 (R1) guidelines. Linearity range of Duloxetine is 50-100 μg/mL and for Pregabalin is 100-200 μg/mL. Correlation coefficient for both drugs was 0.999 and mean recoveries for both drugs were between 99% – 100%, minimum values of %RSD indicate the accuracy of the method. The detailed quantitative results show that this method is sensitive, precise as well as cost...
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