Posted by admin on Mar 31, 2016 in |
The purpose of this study was to investigate the effects of the Ramadan fasting and life rhythm eating rhythm on some elimination parameters, such as the antipyrine clearance, urinary volume and pH. A single oral dose of Antipyrine was administred to 14 healthy volunteers at 08:00 p.m. All the volunteers were submitted to four treatment phases; the first one was carried out 1 week prior to Ramadan (PR), the second on the first week of Ramadan (R1), the third on the third week of Ramadan (R3) and the last one 3 weeks after Ramadan (AR). The salivary kinetic of antipyrine was determined for each treatment throughout the 48 hours following drug intake. Eight urine collections were performed after each antipyrine intake, their volumes and pH were immediately determined. Saliva antipyrine concentrations were determined by high-pressure liquid chromatography (HPLC). No significant differences were found in salivary antipyrine elimination half-life t1/2 and clearance between the four periods studied. The 24 hours urine volume was not significantly modified during Ramadan. However, its...
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Posted by admin on Mar 31, 2016 in |
Recent reports show that germination process increases the hypoglycemic activity of seeds many fold, and can act in a better way for diabetes management. Despite large use of fenugreek seeds than any part of the plant for the treatment of broad range of maladies including diabetes, the anti-diabetic effects of germinated seeds have not been assessed. In the present study, the effect of 21 days oral administration of germinated fenugreek seed extracts (100mg/kg bw, 200mg/kg bw, and 300mg/kg bw) on various biochemical markers like blood, glucose, blood insulin, hepatic glycogen content and key molecular markers of insulin signalling pathway in STZ-induced diabetic rats were assessed. Compared to its respective seed extract, the aqueous sprout extracts from IL8 seed genotype showed significantly higher hypoglycemic activity against the diabetic state and the best dose was established to be 300mg/kg bw. The antidiabetic effect of sprout extracts were quite comparable to standard antidiabetic drug, Voglibiose (1mg/kg bw). The germinated seed extract normalised significantly, various dysregulated biochemical and molecular markers of insulin signalling...
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Posted by admin on Mar 31, 2016 in |
Aim: Present study was aimed to evaluate the analgesic activity of methanolic extract of Crotalaria Burhia roots (Henceforth, MECB) using various experimental animal model of pain. Materials & Methods: Analgesic activity was tested using acetic-acid induced writhing test, formalin test, hot-plate test and tail-flick test for pain in mice and rats. In this study we investigated analgesic activity of MECB at test doses of 100, 150 and 300 mg/kg, p.o. The effects following pretreatment with aspirin, morphine and naloxone were also studied. Standard methodologies were used for the screening of preliminary phytochemical constituents of extract. Results: Result showed significant analgesic activity of MECB in all paradigms. The MECB at the dose 150 mg/kg was shown stronger analgesic activity compared to MECB at the dose of 100 and 300 mg/kg. Moreover, analgesic activity of MECB was note in similar manner to aspirin (100 mg/kg, p.o.) and morphine (5 mg/kg, s.c.). Naloxone (2 mg/kg, i.p.) abolished the analgesic activity of both morphine (5 mg/kg, s.c.) and MECB (150 mg/kg, p.o.) in...
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Posted by admin on Mar 31, 2016 in |
A series of novel cholesteryl lactate-fatty acid conjugates (4a-i) were prepared by esterification of cholesterol with lactic acid, followed by coupling hydroxyl group of cholesteryl lactate with –COOH group of fatty acids including, saturated fatty acids of varying carbon chain of C10-C18, unsaturated fatty acids like 10-undecenoic, oleic ((9Z)-octadec-9-enoic), ricinoleic ((9Z,12R)-12-hydroxyoctadec-9-enoic) and 11-bromoundecanoic acid. Cholesteryl lactate-fatty acid conjugates were characterized by spectroscopic techniques like FT-IR, 1H-NMR, 13C-NMR, ESI-MS and HRMS. All the synthesized compounds were tested for their antimicrobial activity against a panel of seven bacterial strains like Staphylococcus aureus MTCC 96, Bacillus subtilis MTCC 121, Micrococcus luteus MTCC 2470, Klebsiella planticola MTCC 530, Escherichia coli MTCC 739, Pseudomonas aeruginosa MTCC 2453 and fungal strain like Candida albicans. Among them, the compounds with unsaturation (4f and 4h), and functional groups like bromine and hydroxyl group (4g and 4i) showed good antibacterial activity. But, promising activity was observed against Staphylococcus aureus MTCC 96 strain ranging from 7.8-15.6 µg/ml. These compounds (4f-i) are also exhibited good to moderate antifungal activity against...
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Posted by admin on Mar 31, 2016 in |
Crataeva tapia L. (Family- Capparaceae) is an important medicinal tree, commonly called as ‘Varuna’. The plant has been reported to possess several medicinal properties. Propagation of Crataeva tapia L. is mostly by seeds, but germination percentage is very low mainly due to presence of hard seed coat. Conservation and propagation of species using biotechnological tools like plant tissue culture are relevant when natural propagation is hampered. Plant cell culture systems have potential for commercial exploitation of secondary metabolites. These secondary metabolites can then be analyzed using chromatographic techniques. In the present study, a simple, precise, rapid and selective High Performance Thin Layer Chromatography (HPTLC) method was developed and validated for detection and quantification of phytochemical marker β-sitosterol in leaf, bark, leaf callus and stem callus of Crataeva tapia L. as per ICH guidelines....
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