Posted by admin on Mar 31, 2016 in |
Stability indicating RP-HPLC gradient method developed for simultaneous determination of impurities and degradation products from Emtricitabine and Tenofovir Disoproxil Fumarate in pharmaceutical tablet dosage form. The chromatographic separation was achieved by using column ACE C18 (250 x 4.6, 5μ). The mobile phase-A consists of 0.01M potassium dihydrogen phosphate buffer with pH 4.0 adjusted using diluted ortho-phosphoric acid and mobile phase-B as methanol. The flow rate was 1mL min-1 throughout the gradient program with detection wavelength of 270 nm for both components with its related impurities. The column temperature was 30°C and injection volume of 20µl. A well separation of degradation products from main peak and respective known impurities was found with resolution between adjacent peaks greater than 2.0. The method found linear from LOQ to 0.4% level for all impurities with respect to concentration for Emtricitabine(0.7 mg/mL) and Tenofovir Disoproxil Fumarate (1.06 mg/mL) The developed method was validated for specificity, linearity, precision, LOD, LOQ, accuracy and robustness as per ICH guideline Q2A (R1). The results were indicating that the...
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Posted by admin on Mar 31, 2016 in |
A series of six new mixed metal complexes of general formulation [MM12(L)n]xH2O, where M= Mn, Co, Ni, Cu, Zn, Cd,M1=Fe, L= Tartrate ligand, x=1,5,6 and n = 3, are synthesized using tartrate as a bidented ligand. The newly synthesized complexes were characterized using various analytical techniques such as IR, XRD, TGA, Elemental analysis, magnetic susceptibility study etc. IR spectral data suggested that the bidentate ligand has coordination to both the metal ions through oxygen as donor atoms. The thermal behavior (TGA) of the complexes was studied; suggest the decomposition patterns were in good agreement with recommended formulae of the complexes. XRD technique revealed the presence of crystalline nature of all the studied complexes. The antibacterial activity of the metal complexes along with ligand was assayed against three bacterial pathogens viz. Bacillus Megatherium, Staphylococcus aureus, E-Coli, Pseudomonas and Proteus Vulgaris by well diffusion method. The bioassays of all the metal complexes showed a greater inhibitory effect in the form of broad activity spectrum than the individual ligand, which indicate an...
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Posted by admin on Mar 31, 2016 in |
A novel Assay method for the determinationof1-Deoxy nojirimycine(DNJ) was proposed by the authors using ion chromatography. The method was found to be accurate, simple and precise. A method precision RSD of 0.5%, RSD for intermediate precision of 0.6% and correlation coefficient was found to be 0.9999. A recovery of 99.7 to 100.1% at 50%, 100% and 150% levels was found. The DNJ solutions and mobile phase were found to be stable up to 48 hrs. The robustness study was found that the method was robust at low flow, high flow, low strength and high strength. Specificity of DNJ with other cations and organic bases were not interfered. The peak of DNJ was homogeneous, this specificity of peak homogeneity study was performed with UPLC-TOF by collecting peak of DNJ from detector end of Ion chromatography. All analytical parameters were performed by using ICH...
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Posted by admin on Mar 31, 2016 in |
Aim: Evaluation of the antimicrobial, analgesic and antioxidant activity of ethanolic extract of the leaves of Averrhoa Carambola. Materials and methods: To test the antimicrobial activity the organisms used were S. aureus, Klebsiella sp, E. coli and P. aeruginosa, C. albicans. Zone of inhibitions produced by sensitive organisms were demarcated by a circular area of clearing around plant extract impregnated discs and were compared with zone of inhibitions of positive controls. Analgesic activity was tested by two methods: acetic acid induced writhing test and Eddy’s hot plate mediated pain reaction. The animals were divided into 5 groups: Group I (Normal control), Group II (Standard drug), Group III, IV, V (Etahnolic extract of A.carrambola in the doses of 100, 200 and 400mg/kg respectively). To test the antioxidant activity, the mice were divided into 6 groups containing. Paracetamol in the dose of 250mg/kg p.o was administered to all the groups except the first group, which was taken as the normal control, for 10 days to induce oxidative stress. Silymarin in the...
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Posted by admin on Mar 31, 2016 in |
Calotropis gigantea L. flowers were widely used as a natural herbal medication. The objective of the present study was to screen the phytoconstituents and to evaluate the antibacterial and anti-cancer properties of Calotropis gigantea flower extract. The UV-Vis and FTIR spectroscopic techniques were employed to screen the phytochemicals. The antibacterial property of the extract was evaluated through serial broth dilution technique. Cell viability was performed using MTT assay on HeLa cell lines. The presence of Phenolic compounds was confirmed by UV-Vis peak at 271nm. IR spectrum confirms the presence of alcohol/phenols, primary amines, aldehydes, alkanes, alkenes, sulfoxide and halogen compounds. The extract showed antibacterial activity against Enterococcus faecalis (75µg/ml) and Klebsiella pneumonia (2µg/ml). The aqueous ethanolic extract of Calotropis gigantea flower exhibited less activity on HeLa cancer cell viability even at higher concentrations (1000µg/ml). The application of spectroscopic techniques promises to be of a great value in screening phytoconstituents of the extract because of their simplicity and cost-effectiveness. The extract is capable of inhibiting the bacterial growth and may...
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