Posted by admin on Dec 31, 2023 in |
Upgradation of conventional medications with additional therapeutic phytocompounds to combat emerging resistant pathogenic strains, is uptrend now-a-days. The current work investigates the precise in-vitro bactericidal efficacy of the methanolic extract of the unexplored seeds of a swampy weed, Hygrophila auriculata (K. Schum) Heine (MHA), Family – Acanthaceae against different uropathogens. It was observed that, 200µg of MHA has 20% of supplementary inhibitory effect on the antibiotic disc of Amoxicillin-clavulanic acid 30 against Enterococcus faecalis and Escherichia coli. Similarly, MHA treated Nitrofurantoin 300 disc shows 25%, 4.5% and 5% of supplementary inhibitory effect against E. faecalis, E. coli and Staphylococcus aureus, respectively. Ciprofloxacin 5 is supplemented by 2.8% and Streptomycin 10 by 4.5% against S. aureus. 39.28%, 42.39%, 53.36% and 90.4% of increased inhibition was recorded against E. faecalis, E. coli, Pseudomonas aeruginosa and S. aureus respectively in terms of cfu/mL, on treating with a higher concentration of MHA. Present study has noted a minimum inhibitory concentration of 2.5mg/mL of MHA each against E. coli, P. aeruginosa and 1.25mg/mL against...
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Posted by admin on Dec 31, 2023 in |
Introduction: Anxiety disorders are due to reduced serotonin or noradrenaline or GABA in the hippocampus. This study aims at investigating the anti-anxiety actions of verapamil. Materials and Methods: Swiss Albino mice of either sex were used. Group I – control recieved normal saline, Group II fluoxetine 20mg/Kg, Group III Verapamil (10mg/Kg), Group IV Verapamil (20mg/Kg) and Group V- Verapamil (10mg/Kg) and Fluoxetine. Each group had 5 mice. On day 9 of treatment mice were subjected to the marble burying test, light-dark exploration test, act photometer, and social interaction study. Results: Mean (sd) Locomotor activity in the control group was 267.6 (±61.77), 462.6 (± 58.2) with Verapamil 10mg, 561.8 (±33.33) with Verapamil 20mg. Verapamil significantly increased locomotion (P<0.001). The number of entries into light area was 57.20±11.78 in the control group, 85±11.26 with Verapamil (10mg/Kg) and 102.6±7.93 with Verapamil 20 mg/kg. Verapamil significantly increased the number of entries towards light as compared to control mice (P <0.001). The number of entries into dark area was 262.4±16.65 in the control group,...
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Posted by admin on Dec 31, 2023 in |
Background: Having a satisfaction assessment tool that is reliable and valid is paramount in patient satisfaction research. Objective: To develop an instrument to assess the satisfaction of HIV-infected persons covering different aspects of health service delivery in Nigerian HIV treatment hospitals. Materials and Methods: The questionnaire items were developed from literature as well as from key themes identified by Nigerian patients as been most important in the delivery of high-quality care as reported in a systematic review. The following factors were assessed: construct validity, factorial validity, reliability, face and content validity, and practicality. Bishop Shanahan Hospital Nsukka evaluated the instrument’s viability, and a tertiary healthcare facility validated it. Principal components and varimax rotation were utilized in factor analysis. Utilizing internal consistency and Cronbach’s alpha, reliability was examined. Spearman’s rho correlation was used to determine the convergence and discriminant validity. Results: A 10-item questionnaire was developed in a band score of 0-10 response options. ‘’0’’ indicates complete dissatisfaction and ‘’10’’ denotes complete satisfaction. Ninety questionnaires were collected for the pilot...
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Posted by admin on Dec 31, 2023 in |
Avian salmonellosis is a large group of acute or chronic diseases of birds caused by different species of the genus Salmonella. It is a problem of economic concern for all phases of the poultry industry, from production to marketing. The main aim of this study is to investigate the incidence of Salmonella species in the feed and environment of open-system poultry farms. A total of 46 samples were taken from six poultry farms, layers and broilers. The samples include poultry feed from feeders (23 samples) and litter (23 samples). Isolation of Salmonella was carried out in a selective classical medium (DCA) after enrichment in Selenite-F broth. Four Salmonella isolates represent 5% of total samples were recovered; three isolates (75%) from litter samples and one isolate (25%) recovered from water samples; no Salmonellae were recovered from feed samples. All isolates were identified at the species level using cultural characteristics and biochemical reactions. An antimicrobial sensitivity test for the four Salmonella isolates was carried out. Each isolate was tested to 10...
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Posted by admin on Dec 31, 2023 in |
A simple, novel, precise, rapid, accurate, specific reverse phase high performance liquid chromatography (RP-HPLC) method for estimation of Teneligliptin hydrobromide hydrate and Dapagliflozin propanediol monohydrate API in marketed formulation was developed and validated. The separation was carried out on a Prontosil C18 (250 x 4.6 mm, 5 m) column using an acetonitrile: 3.5 pH Potassium dihydrogen phosphate buffer (60:40 v/v) mobile phase. The flow rate was 1 mL/min, and the UV detector was set to detect at 227 nm. Teneligliptin Hydrobromide Hydrate retained 2.37 min, while Dapagliflozin Propanediol Monohydrate retained 3.61 min. Teneligliptin hydrobromide hydrate revealed a linear response in the concentration range of 10-30ppm. In the concentration range of 5-15ppm, dapagliflozin propanediol monohydrate demonstrated a linear response. Teneligliptin hydrobromide hydrate and Dapagliflozin propanediol monohydrate had correlation coefficients (‘R2‘ value) of 0.999 and 0.992, respectively. Various validation parameters were used to validate the analysis results. Teneligliptin hydrobromide hydrate and Dapagliflozin propanediol monohydrate percentage recoveries vary from 98% to 101%. Both precision and robustness study found to have % RSD...
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