Posted by admin on Dec 31, 2015 in |
Many microorganisms thrive on the abundant nutrients in the rhizosphere and they produce antagonistic metabolites. The present study is an attempt to investigate the potent bacteria producing antibiotics against pathogenic microorganisms from the rhizosphere soil samples of Kanjamalai hills, Salem district, Tamilnadu, India. A total of 6 isolates of bacteria was obtained and screened for inhibitory activity against selected opportunistic pathogens. Potent bacterial isolate (A6) was identified as Pseudomonas aeruginosa by cultural, morphological and biochemical characterization. The antimicrobial activity of broth culture of Pseudomanas aeruginosa was also observed. The antimicrobial active compound was partially purified from the broth culture and its concentration was estimated to be 373.086 µg/ml. The obtained protein was separated in SDS-PAGE and its molecular weight was determined as 66.4 kD, 44.3 kD, 29.0 kD, 20.1 kD and 14.3 kD using standard molecular markers. The results showed that the isolated strain has high potential against Bacillus megaterium and Aspergillus niger. It can be further investigated to identify the antibacterial compounds produced and their mechanisms of...
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Posted by admin on Dec 31, 2015 in |
A pessary is a type of suppository that is meant to be inserted into the vagina for treatment of local or systemic diseases, in folk medicine garlic bulb is inserted into vagina for treatment of candidiasis. The aim of this work is to formulate and evaluate aqueous extract of garlic as a pessary. Aqueous garlic (Allium sativum) extract was obtained from the garlic bulbs. The pessary was formulated using the pouring method and cocoa butter as a base. The formulated pessaries were evaluated using physical appearance, crushing strength test, disintegration and dissolution times tests as well as content uniformity. The visual examination showed brilliant and smooth surfaced, consistently bullet shaped, light brown colour and mild garlic odour. The results of crushing strength (3.96 ± 0.92 KgF), disintegration time (5.17 ± 0.17 min) and content uniformity (96.03 ± 7.26 %) were all within the normal limits. The result obtained from the dissolution time test showed that the pessary dissolved within 30 min. The results indicated that aqueous garlic extract can...
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Posted by admin on Dec 1, 2015 in |
In Advances drug design technologies, a large no of drug molecules are being introduced in every year but many of these molecules have problems like their solubility, stability, bioavailability and its long lasting side effects. Low bioavailability is one of the serious but curable problems in case of the drug molecule. Low bioavailability happens because a lot of molecules unable to permeation the gastrointestinal epithelia. There are some other factors also which responsible for low bioavailability i.e. low lipophilicity and zwitterionic character at physiological pH, poor water solubility or efflux by P-glycoprotein (P-gp) etc. The object of this review is to explore the concept of bioavailability to achieve better therapeutic response in appropriate dose using natural drugs and natural products like ginger, caraway, aloe, quercetin, glycyrrhizin piperine, curcumin etc. The use of natural products is the most reliable means for bioavailability enhancement because these are safe, non-toxic, economical, easily procured, non-addictive, pharmacologically inert and non-allergenic in nature etc. This review explores the natural drugs from plant and animal sources...
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Posted by admin on Nov 30, 2015 in |
Context: The leaves Morus alba have been traditionally used in Asian countries for treatment of different ailments. Objective: Present study was undertaken to evaluate the effect of ethanol extract from Morus alba leaves supplementation on gastric tissue glutathione level in indomethacin induced ulcers in rats. Materials and Methods: Ethanol extract from Morus alba leaves were administered at the doses 200 and 400 mg/kg/day, p.o. for seven days. Ulceration was induced by giving indomethacin. Ulcer index, gastric GSH level, gastric wall mucus level was estimated. The functioning of liver was also assayed. Results: The effects of ethanol extract from Morus alba leaves were significant and comparable to reference treated group of rats. The gastric GSH levels were replenished by Morus alba leaves in 400 mg/kg. Conclusion: From this study it was concluded that ethanol extract from Morus alba leaves possess significant ulcer protection by replenishing gastric GSH and increasing gastric mucus...
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Posted by admin on Nov 30, 2015 in |
The present study involves the investigation of anti-inflammatory activity for 2’, 4’ dihydroxy flavone by TPA [12- O- Techanoyl 13 – Myristate] induced inflammation in Polymorphonuclear leukocytes [PMNL]. The cell viability was assayed by Trypan blue dye exclusion assay, MTT assay, release of Cathepsin D, Nitrite and TNF-α levels. Diclofenac sodium was used as the standard drug. The selected dihydroxy flavone, 2’, 4’ dihydroxy flavone showed the significant increase in the number of viable PMNL cells and decreases the release of Cathepsin D, Nitrite and TNF-α. The present work evidenced the in-vitro anti-inflammatory effect of 2’, 4’ dihydroxy...
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