Posted by admin on Jan 31, 2016 in |
A new single stability-indicating isocratic reversed phase ultra-performance liquid chromatographic (RP-UPLC) method with a shortest run time of 2.5 minutes is developed for the determination of assay of bicalutamide an anti-cancer drug from its finished dosage form in the presence of its forced degradation products. The method is developed using Waters Aquity BEH C18 column with mobile phase containing an isocratic mixture of solvent A and B. The eluted compounds were monitored at 270 nm. Bicalutamide tablets were subjected to the stress conditions of oxidative, acid, base, hydrolytic, thermal, and photolytic degradation, within which forced degradants were well-resolved from main peak, thus proving the stability indicating power of the method. The developed method is validated as per ICH guidelines with respect to specificity, linearity, Accuracy, precision and robustness. The same method is also tested for evaluation of dissolution where recovery, linearity and solution stability were evaluated and found satisfactory. As a part of cleaning validation-Residue analysis, recovery of 0.5ppm on two different surfaces (Stainless steel and Class-A Glass) were...
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Posted by admin on Jan 31, 2016 in |
Sustained release Carvedilol tablets constituting reservoir pellets developed in this study is an attempt to investigate the effect of drug release from matrix. The sustained release Carvedilol tablets were prepared by using different combination with release modifier (ethyl cellulose 10 cps and Eudragit RS 100). Tablets containing disintegrant pellets (Crosspovidon) with active ingredient demonstrated a sustained rate of drug release. The Carvedilol tablets were prepared using different ratios of pellets. After 12 hours of dissolution study, Carvedilol release from the matrix systems were 92.35%, 92.37%, 95.25%, 93.26%, 92.87% and 90.4 2% from formulation F1, F2, F3, F4, F5 and F6 respectively. Formulation F2 (10% of Eudragit RS 100) exhibited 95.21% Carvedilol release in 12 h and satisfied all the physical evaluation parameters hence, considered as optimized batch. The Carvedilol sustained release F2 batch showed non-Fickian diffusion...
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Posted by admin on Jan 31, 2016 in |
Momordica cymbalaria Fenzl (Cucurbitaceae) fruit powder and extracts are reported to have Type 1 antidiabetic activity in experimental diabetic models. The objective of this study was to investigate the neuroprotective action of an oleanane-type triterpenoid saponin isolated from the plant in diabetic peripheral neuropathy (DPN) by in vivo and in vitro methods. Male Wistar rats were rendered diabetic by administration of streptozotocin intraperitoneally. The rats were maintained for six weeks to induce neuropathy. Treatment groups received the saponin and epalrestat, an aldose reductase inhibitor. At the end of treatment, the animals were evaluated for various parameters of neuropathy like muscular grip strength and pain sensation tests using hot plate and tail-flick methods, and nerve conduction velocity (NCV) measurement. In vitro models of DPN were created by initiating sciatic nerve cultures of rats in 12-well plates. The nerve cultures were then rendered diabetic by exposing them to high glucose. After incubation of 7 days, the tissues were assayed for aldose reductase (AR) and sorbitol dehydrogenase (SDH) activities. Intraneuronal accumulation of...
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Posted by admin on Jan 31, 2016 in |
In the present study, Marumoside A isolated from Moringa oleifera, a traditional medicinal plant, was synthesized for the first time using trichloroacetimidate donor as a key step. The aglycone 4-hydroxy phenylacetamide was prepared from 4-hydroxyphenyl acetic acid using oxallyl chloride and aqueous ammonia. The lipid derivatives of Marumoside A were synthesized using different fatty amines. The anti-inflammatory activity of the Marumoside A and its lipid derivatives was evaluated for the inhibition of TNF-α and IL-1β secretion levels. Among all the synthesized molecules, the oleoyl amine lipid (unsaturated) derivative showed significant inhibition of TNF-α and IL-1β secretion with IC50 value of 16.7 µM and 23.4 µM, respectively, when compared to Marumoside A and its saturated lipid derivatives. Further, the Marumoside A and its lipid derivatives also showed DPPH radical scavenging...
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Posted by admin on Jan 31, 2016 in |
Quetiapine is indicated for the treatment of schizophrenia as well as for the treatment of acute manic episodes associated with bipolar I disorder. The aim of the present study is to evaluate the effect of manufacturing process on critical quality attribute on In-process parameters (Granules). Quetiapine fumarate was selected as a model drug for sustained release drug delivery system manufactured by wet granulation process 1. To identify the effect of compacted Magnesium Oxide Light in the release behavior of model drug. During the study different percentage of Magnesium Oxide light compacted and granulated by using dichloromethane as granulating solvent. Other excipients with different functionality were added in the formulation such as Microcrystalline Cellulose, Lactose monohydrate, Controlled release polymer Carrageenan, Povidone and Magnesium stearate. Initially Magnesium Oxide light was compacted by using vertical type Roller compactor. From the compacted material 40%- 50% screened & 50%-60% retained over #60 mesh is optimized ratio, and this ratio must be maintained to get the targeted release of the said drug from the final...
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