Posted by admin on Mar 1, 2016 in |
Cefixime is more effective in treating multi drug resistant typhoid fever but the major limitation was its poor bioavailability with short half- life. The present investigation was attempted to develop a natural polymer based nanoparticles containing cefixime using gum kondagogu and chitosan as polymers for customising the drug release profile to achieve an effective therapeutic concentration by increasing its solubility and bioavailability. The cefixime nanoparticles were prepared by the modified coacervation method and evaluated for particle size, zeta potential, morphological studies (SEM, TEM and AFM), entrapment efficiency, in-vitro drug release studies and in-vitro antimicrobial efficiency studies. The prepared drug nanoparticles found to be average mean particle size in range from 80.6±3.5 to 230.6±7.3nm and entrapment efficiency was found to be 78.0±1.5 to 93.2±2.5%. The in-vitro drug release showed a biphasic pattern with initial burst release followed by sustained drug release up to 32h. The MIC50 of prepared formulation was one fold lesser than pure cefixime for Salmonella Typhi isolates. The disc agar diffusion (DAD) test confers that the zone...
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Posted by admin on Mar 1, 2016 in |
The compound 3-(1-(benzo[d]thiazol-2-ylimino) ethyl)-6-methyl-2H-pyran-2, 4(3H)-dione (3BTIEMPD) and its metal complexes were prepared and characterized by spectro-analytical techniques. Irving-Rossetti pH-metric technique was employed to determine the number of dissociable protons and chelation sites of the title compound. Hyper Chem 7.5 software was used to understand donor and acceptor properties of molecule. The molecule was built and the geometry optimization was carried out by using semiemperical PM3 method. The contour maps of highest occupied molecular orbitals (HOMO) and lowest unoccupied molecular orbitals (LUMO) and corresponding binding energy values were computed. The low binding energy for ionic species evident from computed values is an indication of higher possibility of compound to bind with metal ions through deprotonation. This is augmented from the results of spectro-analytical analyses of isolated solid metal complexes wherein the participation of azomethine nitrogen and the enol oxygen of pyran ring through deprotonation is envisaged. All the compounds were investigated for their antimicrobial activities against the Gram-positive and Gram-negative bacteria by disc diffusion method. It was found that the...
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Posted by admin on Mar 1, 2016 in |
The present work has been under taken to study the antibacterial activity of stem, leaf, flower and seed extracts of Trichosanthes cucumerina L against disease causing bacteria. Antibacterial activity of different solvent extracts (Aqueous, Methanol, Chloroform, Petroleum ether, Acetone) of stem, leaf, flower and seed of T. cucumerina has been studied to find out their activity against nine pathogenic bacteria viz., Bacillus cereus, Micrococcus luteus, Escherichia coli, Proteus vulgaris, Klebsiella pneumonia, Bacillus sphericus, Salmonella typhimurium, Pseudomonas aeruginosa and Staphylococcus aureus. The antibacterial activity of the extracts of different plant parts was done through well diffusion method and by measuring the inhibition zone around the disc. According to our observations, the seed extracts of T. cucumerina exhibited antibacterial activity against all the bacteria under study. The results provided evidence that the species T. cucumerina can be used as a potential source of antibacterial...
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Posted by admin on Mar 1, 2016 in |
The main objective of this present work was to develop and evaluate matrix tablets of Atenolol by melt granulation technique and compare the drug release between bess wax and glyceryl monostearate (GMS). Polymers like HPMC K 4 M, HPMC K 100 M, bees wax, glyceryl monostearate and ethyl cellulose were incorporated in the formulation. Formulations were prepared by keeping the HPMC and ethyl cellulose at constant ratio. Bees wax and glyceryl monostearate (GMS) were used in different ratios. FTIR studies indicate that there is no interaction between drug and excipients. Developed tablets were evaluated for weight variation, friability, hardness, thickness, drug content, swelling index and drug release profiles. The dissolution studies showed that drug release was better in the formulations where glyceryl monostearate (GMS) is higher than the bees wax. Thus, it was concluded that HPMC K 4 M, HPMC K 100 M along with Bees wax and Glyceryl monostearate can be successfully used in the formulation of Atenolol sustained release gastro retentive floating drug delivery...
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Posted by admin on Mar 1, 2016 in |
Cancer is primarily an environmental disease with 90–95% of cases attributed to environmental conditions and 5–10% due to genetics.RAS proteins act as binary molecular switches and play an important role in intracellular signal transduction which regulates processes such as actin cytoskeletal integrity, proliferation, differentiation, cell adhesion, apoptosis, and cell migration. The present bio-computational analysis was performed using web-based tools and servers. Multiple sequence alignment of selected human RAS subfamily proteins with other homologous sequences revealed highly conserved regions. The present work determined the physico-chemical properties of selected RAS proteins such as their hydrophilic nature; alpha–helical structure and close evolutionary relationship with higher vertebrates. Cancerous proteins (gi166706781, gi 4505451) from human having most of homology with the number of species. On the basis of this study we can suggest that these organisms can be taken for the further study and after the successful analysis, these can be implemented on...
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