Posted by admin on Jan 31, 2016 in |
Background: The WHO assembly in number of resolutions has emphasized the need to ensure quality control of medicinal plant products by using modern techniques and applying suitable standards. Since Ayurvedic medicines come under the purview of Drugs and Cosmetics Act, there is increased general awareness about the necessity for developing standards for the purpose of quality control by the manufacturers as well as by the Drug control Authorities and for quality assurance to the public. Standardization of Ayurvedic formulations is an important step for the establishment of a consistent biological activity, a consistent chemical profile, or simply a quality assurance program for production and manufacturing of herbal drugs. Aim: To standardize Ashwagandha ghrita. Materials and methods: Physico‐chemical studies like Refractive index, Specific gravity, Rancidity, Boiling point / Melting point, Acid value, Saponification value, Iodine value and HPTLC were carried out as per the WHO guidelines, Ayurvedic Pharmacopoeia and Indian Pharmacopoeia. Conclusion: Standardization tests done on Ashwagandha ghrita helped in authenticating and ensuring the quality of the...
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Posted by admin on Jan 31, 2016 in |
The objective of present study was to develop Lornoxicam transdermal gel and its iontophoretic delivery to enhance its permeation for systemic effect and to avoid its side effects. Lornoxicam is a COX-1 and COX-2 inhibitor used in the treatment of inflammation, pain, edema and rheumatoid arthritis. Transdermal gel of Lornoxicam was formulated using triethanolamine as solvent, carbopol 934P as gel base and enhancement in its permeation by iontophoresis was investigated. Formulated gel was evaluated for pH, viscosity, spreadability, drug content and gel strength. Permeation study was carried out using cellophane membrane for 6 hours. Anti- inflammatory activity of Lornoxicam gel was studied in albino rats by carrageenan induced paw edema method in which Lornoxicam was delivered through rat’s skin by passive delivery and iontophoretic delivery. Iontophoretic delivery of formulation F1 showed edema inhibition up to 62% whereas passive delivery of F1 showed 22% of edema inhibition in 1 to 6 hours. Edema inhibited by iontophoretic delivery was around 2.5 fold than compared to that of passive delivery; hence iontophoretic...
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Posted by admin on Jan 31, 2016 in |
A galactose-specific lectin was purified from seeds of a leguminous plant, Bauhinia variegata, by affinity chromatography on lactose–agarose. Protein extract agglutinated human erythrocytes (treated with proteolytic enzyme). Among various carbohydrates tested, the lectin was best inhibited by D-galactose and other sacharides. SDS-PAGE showed that the lectin, named BVL produce a single band establishing that the lectin is composed of similar type of subunits. The antimicrobial activity of the purified lectin was carried out by agar ditch diffusion method at different concentrations using appropriate standards. BVL demonstrated a remarkable antibacterial activity against the pathogenic bacteria Pseudomonas aeroginosa, Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. BVL also shows a significant antifungal activity against Aspergilas niger and Penicilium...
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Posted by admin on Jan 31, 2016 in |
Crustacean’s shells constitute the traditional and current commercial source of Chitin. These derivatives/molecules are potential resource as well as multiple functional substrates have generate attractive interest in various fields such as biomedical, pharmaceutical, food and environmental industries. In the present investigation, chitinous wastes were collected from the fresh water areas of Dehradun and Rishikesh of Uttarakhand State. The bacterium, Bacillus sp. isolated from soil produces chitinase enzyme responsible for degradation of chitin obtained from chitinous wastes. Further the chitinases enzyme was utilized to degrade the chitinous wastes into chito-oligosaccharides. Chitosan was prepared by deacetylation process and chito-oligosaccharides (COS) was prepared by enzymatic hydrolysis of chitosan. As per the solubility aspect of chitosan and chito-oligosaccharides, it was found that these are more soluble in comparison to chitin, the parent compound. The anti-diabetic potential of these chitin based derivatives was determined against alloxan-induced albino rats (diabetic model). In alloxan-induced diabetic rats, both the chitosan and chito-oligosaccharides (COS) both decremented blood sugar levels at the cessation of 1st, 2nd and 4th hours...
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Posted by admin on Jan 31, 2016 in |
Diabetes mellitus is known to induce microvascular changes and alterations to neuronal functions. Diabetic neuropathy and diabetic nephropathy are two of the major long-term complications of diabetic mellitus (DM). The main aim of the comparative study is to investigate the role of cations contents compared to the age matched control.Adult rats were humanely killed and detrusor muscles of urinary bladder located and excised rapidly and placed in organ baths. Then urinary bladder detrusor muscles were blotted, weighed and dissolved in concentrated nitric acid for the measurements of cation contents. The levels of Na+, K+, Mg2+, Zn2+, Cu2+, Ca2+, Pb2+ and Fe2+ were measured by flame photometry, atomic absorbance spectrophotometry and inductively coupled plasma- mass spectrometry. The results show marked changes in the characteristics of diabetic and control animals. Diabetic rats weighed significantly (P<0.05) less compared to age-matched control rats. Diabetic rats also have significantly (P<0.05) elevated blood glucose, weight of bladder and bladder strip compared to age-matched control rats. The results also show that the levels of cations for...
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