Posted by admin on Jan 31, 2016 in |
Acute Lymphoblastic Leukemia (ALL) is one of the deadly diseases among children causing a number of deaths worldwide. It could be treated using chemotherapy, bone marrow transplantation or irradiation therapy but it may cause side-effects. Therefore, interest has arisen in the use of plants for treatment of ALL. Cannabis sativa is known to produce L-asparaginase which is used to treat ALL. L-asparaginase breaks down asparagine to aspartic acid and ammonia. Thus the cancer cells die due to asparagine starvation as they cannot synthesize asparagine on their own. L-asparaginase was extracted from C. sativa and immobilized to develop a biosensor for the detection of asparagine levels in leukemic serum samples. Out of the various immobilized techniques Polyvinyl Alcohol and hydrosol gel on nylon membrane were found to give the fastest response. Therefore, they were used to detect asparagine levels in leukemic serum samples. The concentration of asparagine in leukemic samples was 10-2 to 10-3 M and in normal samples it was 10-5...
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Posted by admin on Jan 31, 2016 in |
Traditional knowledge and ethno-botanical use of plants have been widely acknowledged all over the world. The cicatrizing properties of extracts obtained from this plant have been scientifically studied, attributing the main biological activity to its tannin and flavonoid content. Recent commercialization of the plant drug Adiantum capillus veneris Linn. requires pharmacognostical information to develop quality-control methods for raw materials and extracts produced with this plant drug. Macro and micro-morphological parameters were established to authenticate the genuine drug that allowed detection of adulterants usually found in commercial samples of this plant material. All the parameters were studied according to WHO guidelines and Indian Pharmacopoeia. These morphological characteristics can be used for rapid identification of the drug and are particularly useful in the case of powdered materials. Physicochemical parameters such as total ash, water soluble ash, acid insoluble ash, sulphated ash, crude fibre content, loss on drying, percentage of foreign matter and extractive values were determined. Preliminary phytochemical screening in different solvents showed the presence of steroids, flavonoids, terpenoids, fats, tannins...
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Posted by admin on Jan 31, 2016 in |
The periodontal tissues undergo constant changes of remodelling when subject to bacterial infection and the resultant host response. The balance between enzymes secreted by the host such as matrix metalloproteinases (MMPs) and their antagonists tissue inhibitor of metalloproteinases (TIMPs) are major contributors to the progression of periodontal destruction. A sub-type of MMPs referred to gelatinases (MMP-2 and 9) are known to be responsible for the pathologic tissue degradation, with MMP-9 playing a potential role in periodontal pocket formation in periodontitis. The use of anti-collagenase host modulatory agents such as doxycycline hydrochloride regulates the extent of resultant tissue destruction and provides an ideal therapeutic approach in the treatment of periodontitis. However, data regarding the effect doxycycline hydrochloride on gelatinases has not been extensively documented. Therefore, the aim of this study was to identify the expressions of MMP-2 and 9 by an immunohistochemical assay in experimentally induced-periodontitis animal models and to evaluate the effects of a prepared doxycycline hydrochloride-impregnated collagen scaffold on their...
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Posted by admin on Jan 31, 2016 in |
The present study was carried out to explore anti-diabetic, antidyslipoproteinemic and anti oxidant activities of Tinospora cordifolia (Menispermaceae) in type 2 diabetic patients. In this study clinical trial was conducted on type 2 diabetic patients attending the diabetes OPD, at Kaya Chikitsha Vibhag, Rajkiya Ayurvedic Chikitsha Mahavidyalaya, Touriya Ganj, Lucknow, following all ethical guide lines. All biochemical assays were carried out by the standard kit methods. A marked increase in plasma levels of fasting blood sugar, lipid profile accompanied with increase in the lipids and apo-protein levels of serum β lipoproteins following decrease in lipid and protein constituents of α lipoprotein were noted in type 2 diabetic patients compared to healthy controls. The results after prescription of Tinospora cordifoia (powered stem; 50 mg/kg body weight, p. o.) for 15 days in type 2 diabetic patients resulted significant decrease in the level of fasting blood sugar, total cholesterol, β lipoproteins and triglycerides. The decrease in lipids and apoprotein levels of β lipoproteins was accompanied with stimulation of plasma lecithin cholesterol...
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Posted by admin on Jan 31, 2016 in |
The aim of the present study is to probe the antimicrobial activities of methanol leaf extract of Leucas sp. Antifungal susceptibilities of certain clinically isolated dermatophytes to methanol extracts of Leucas aspera and Leucas zeylanica leaves were performed using agar well diffusion method. The result obtained shows that all the extracts expressed remarkable antifungal activity with zone of inhibition ranging from 5 to 10mm. Maximum inhibition zone was recorded with Penicillium sp. (10 mm) while minimum inhibition zone of was recorded for Candida tropicalis (3 mm). From the study it is evident that Leucas aspera and Leucas zeylanica possess potential antidermatophytic activity and further study on these plants may lead to explore novel bioactive...
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