Posted by admin on Dec 31, 2015 in |
Salbutamol is a selective β2-adreno-receptor stimulant and is given four times daily in a dose of 2.4 mg orally to maintain therapeutic blood level. It is known to be absorbed in proximal parts of the small intestine. Prolonging gastric residence time is, therefore, beneficial to improve its bioavailability. Hence, seven in situ gelling liquid formulations were prepared using sodium alginate (SA) as gel forming agent and HPMC as viscosity enhancer. CaCO3 was incorporated as cross-linker and floating agent. Gelling capacity was evaluated based on a graded response which indicates rapidity of gelation and time taken by the gel to dissolve. All the formulations showed instant gelation but with regard to integrity, all formulations except the one with lowest SA level formed stiff gels maintaining integrity for at least 12 hr. With respect to floating behavior, all formulations except the one with the lowest SA level floated for more than 12 hr irrespective of their composition. Most of the formulations took less than 1 min to float but those with...
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Posted by admin on Dec 31, 2015 in |
The aim of present study was to examine the analgesic, neuropharmacological and cytotoxic effects of the methanolic extract of the barks of Dillenia indica Linn. The analgesic activity was evaluated for its central and peripheral pharmacological actions using tail-flick method and acetic acid-induced writhing test in mice respectively. The extract, at the dose of 100, 200 and 400 mg/kg body weight, produced a significant increase in pain threshold in tail-flick method in a dose dependent manner. In acetic acid-induced writhing test, the extract at 400 mg/kg dose showed a maximum of 46.0 % inhibition (p <0.001) of writhing reaction compared to the reference drug Diclofenac-Na (78.50%). The extract was also investigated for its neuropharmacological action using hole-cross and open field test. The extract displayed dose dependent suppression of motor activity and exploratory behavior in mice in the tested models. The results of the study indicate that the plant possesses strong analgesic potential which might be linked to inhibition of central mechanism of pain. However, studies are required on higher...
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Posted by admin on Dec 31, 2015 in |
A novel (N’, N”E, N’, N”E)- N’, N” – (2, 2′ – (propane-1,3-diylbis(sulfanediyl))bis(1-(4-chlorophenyl) ethan – 2 – yl – 1 -ylidene))bis(2-hydroxybenzohydrazide)(CAPSH) is identified as a sensitive and selective analytical reagent for the determination of trace levels of Co(II) in environmental samples like in food, water, synthetic samples and medicinal plants by spectrophotometry. CAPSH reacts with cobalt(II) at pH 7.0, in aqueous DMF to form orange colored 1:1 (Co-CAPSH) complex shows maximum absorbance (λmax) at 380nm. The color reaction is immediate and the absorbance remains constant for about 24h. The molar absorptivity coefficient and Sandell’s sensitivity are found to be 7.9×104 L.mol-1 cm-1 and 0.048μg cm-2 of cobalt(II) respectively. The linear regression coefficient is 0.9998 and standard deviation is 0.0050. The system obeys Beer`s law in the range of 00.059-0.59μg mL-1of cobalt(II). Large number of cations, anions and complexing agents do not interfere in this method. This method is successfully applied for the determination of cobalt(II) in environmental samples like food, water (potable and polluted), synthetic samples and medicinal...
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Posted by admin on Dec 31, 2015 in |
Acetylcholinesterase (AChE), a member of α/ß hydrolase protein super family, is the key enzyme in the breakdown of acetylcholine (ACh) – an important neurotransmitter. Inhibition of this enzyme has been considered an auspicious strategy for the treatment of various neurological disorders such as Alzheimer’s disease (AD). In this study, crude methanolic Ipomoea muricata seed extract was subjected for analysis of its potential AChE inhibitory activity using a microplate assay based on Ellman’s method. I. muricata’s inhibitory activity was compared using Donepezil, a prescription drug for AD treatment. Two time intervals were used: 40 minutes and 24 hours. Based on the findings, Donepezil exhibited greater potency (IC50 > 0.005 μg/mL) at both time intervals in contrast to I. muricata (IC50 = 39.67 μg/mL) at 40 minutes and (IC50 > 57.44 μg/mL) at 24 hours. It is important to note that the crude methanolic seed extract of I. muricata has been shown to cause moderate but persistent inhibition of AChE in comparison to Donepezil which has shown a strong but short-lived...
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Posted by admin on Dec 31, 2015 in |
Many microorganisms thrive on the abundant nutrients in the rhizosphere and they produce antagonistic metabolites. The present study is an attempt to investigate the potent bacteria producing antibiotics against pathogenic microorganisms from the rhizosphere soil samples of Kanjamalai hills, Salem district, Tamilnadu, India. A total of 6 isolates of bacteria was obtained and screened for inhibitory activity against selected opportunistic pathogens. Potent bacterial isolate (A6) was identified as Pseudomonas aeruginosa by cultural, morphological and biochemical characterization. The antimicrobial activity of broth culture of Pseudomanas aeruginosa was also observed. The antimicrobial active compound was partially purified from the broth culture and its concentration was estimated to be 373.086 µg/ml. The obtained protein was separated in SDS-PAGE and its molecular weight was determined as 66.4 kD, 44.3 kD, 29.0 kD, 20.1 kD and 14.3 kD using standard molecular markers. The results showed that the isolated strain has high potential against Bacillus megaterium and Aspergillus niger. It can be further investigated to identify the antibacterial compounds produced and their mechanisms of...
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