Posted by admin on Jan 1, 2016 in |
Aim: To demonstrate the effect of Nimesulide in reversing Reserpine induced depression in mice. Materials and Methods: This test was done using 20-25gm of healthy albino mice of either sex in the Department of Pharmacology of Gauhati medical college. They were housed in standard laboratory condition at 25ºC and fed on standard diet and water ad libitum. Five groups were selected each containing six mice. The groups were Group I (Normal Control), Group II (Disease Control), Group III (Fluoxetine 10mg/kg i.p),Group IV ( Nimesulide 2.5mg/kg i,p) and Group V (Nimesulide 5mg/kg i.p)Reserpine (2mg/kg i.p) was used to induce depression in all the groups except the Normal Control. After 24 hours, the standard and test drugs were given and the mice were subjected to tail suspension test (TST) after 30 mins and forced swim test (FST) after 1 hour of injecting the drugs. Result: Mean±SEM values were calculated for each group. The data were analyzed using ANOVA and post analysis was done by Dunnett’s test. Results were found to be...
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Posted by admin on Jan 1, 2016 in |
The present investigation was attempted in order to evaluate the antifungal potential of successively extracted n-hexane and methanolic extracts of Melia azedarach L. bark. The fungi-toxic efficacy of the extracts were tested against both yeast like fungi [Candida albicans (MTCC-3017), Candida krusei, Candida tropicalis, Cryptococcus marinus (MTCC-1029)] as well as mycellial like fungi such as Aspergillus niger (MTCC-9933) and Rhizopus oryzae employing well diffusion and poison food method respectively. The study revealed that n-hexane extract exhibited high inhibition against Candida krusei, whereas Candida albicans and Candida tropicalis showed no response and Cryptococcus marinus responded moderately. The methanolic extract showed high inhibition against Candida krusei, whereas Candida albicans and Candida tropicalis showed no response and moderate effect against Cryptococcus marinus. The n-hexane and methanolic extracts of this plant showed better zone of restriction against Rhizopus oryzae than Aspergillus niger. Fluconazole and clotrimazole was taken as reference antifungal. The result clearly indicated that bark of Melia azedarach L. contains phytochemicals which are responsible for inhibition of fungal growth and...
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Posted by admin on Jan 1, 2016 in |
Boerhavia erecta is a weedy herb of the family Nyctaginaceae and is commonly available in almost all places. The present study is so designed for phytochemical screening of the plant as well as its antibacterial and antioxidant activities of the root extract of B. erecta. The whole plant was collected in and around Chennai. Phytochemical screening of acetone extract of B. erectarevealed the presence of flavonoids, tannins, phlobatannin, saponin, terpenoids, glycosides. The antibacterial activity of the crude extract was determined by the well diffusion method against four pathogenic bacteria. At higher concentratio the extract showed good zone of inhibition against E.coli and P. aeruginosa. DPPH assay, Reducing power and Hydroxyl radical scavenging assay of acetone extract was studied and compared with standard Quercetin and Ascorbic...
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Posted by admin on Jan 1, 2016 in |
Simple and rapid Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) and UV-spectrophotometry method was developed for the determination of Paracetamol, Pamabrom and Dicyclomine hydrochloride, detection was carried out using photo diode array detector. Chromatographic separation of the analytes was achieved within 3.12, 4.25, 5.35 min for Paracetamol, Pamabrom and Dicyclomine hydrochloride by LC-GC Qualisil Gold-C18 (250 x 4.6 mm i.d., 5µm) column, mobile phase was methanol : water (1% TFA with pH adjusted to 3.0 with ammonia) in the ratio of (83:17), 0.4% (v/v) TEA and was filtered and degassed., flow rate was 1.0 mL/min, and the detection was carried out at 221 nm. Calibration curve was linear (r2=0.9993) in the range of 13-78 g/mL for Paracetamol, r2=0.9991 in the range of 1-6 g/mL for Pamabrom and r2=0.9993 in the range of 250-1500 g/mL for Dicyclomine hydrochloride. The commonly used excipients and additives present in the pharmaceutical formulations were free from interfering components, as illustrated by Specificity of the method. Accuracy was performed as recovery study and results showed the...
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Posted by admin on Jan 1, 2016 in |
Objective: The aim of this current research is to study a novel carrier, niosome, for its enhanced transdermal delivery of tenoxicam. Methods: Niosomes are self assembled vesicles composed primarily of synthetic surfactants, cholesterol & edge activator (surfactant). Niosomes were prepared by Organic solvent injection technique and characterized by particle size, entrapment efficiency, transmission electron microscopy (TEM), ex-vivo skin corrosive test, in-vitro dissolution, ex-vivo permeation studies and in-vivo study. Results: Vesicle size and % EE of the optimized niosomal formulation was found to be 79.46 ± 55 nm and 88.8 ± 4.5% respectively. Zeta potential of the optimized niosomal formulation was found to be -39.6 ± 7.43 mv. The ex-vivo study showed transdermal flux of niosomal gel to be 43.09± 0.106µg/cm2/h which was 2.9 fold higher than that obtained after application of plain drug gel whose flux was 15.05±0.09 µg/cm2/h). Conclusions: Data obtained from this experimental work concludes niosomal formulation is safe, very effective and promising drug carrier for transdermal delivery of...
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