Posted by admin on Jan 1, 2016 in |
A simple sensitive, accurate, precise and stability indicating high performance thin layer chromatographic method has been developed and validated for the estimation of troxipide in bulk drug and formulation. The method was developed using silica Gel 60 F254TLC plates, with hexane: ethyl acetate: methanol: water: ammonia 1: 5: 5: 1.5:0.2 (v/v) as mobile phase. The densitometric scanning was performed at λ = 263 nm. The method was validated for linearity, accuracy, precision, specificity and robustness according to ICH guidelines. The calibration plots were linear in the range 200-700 ng/band with r2= 0.998. The LOD and LOQ were found to be 20.39 and 61.81 ng/spot, respectively. Troxipide was subjected to acidic, alkaline hydrolysis, oxidation, photo degradation and thermal degradation. The drug undergoes degradation in acidic as well as alkaline hydrolysis and thermal degradation; however it was found to be relatively stable for photolytic and oxidative degradation conditions. As the drug peak and the degradation product peaks were well separated from each other; this method can be employed as stability indicating...
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Posted by admin on Jan 1, 2016 in |
Decalepis hamiltonii Wight and Arn. is an indigenous medicinal plant, which has a folk reputation in Western and southern India as hypoglycemic agent. The present investigation was carried out to evaluate the antidiabetic effect of ethanolic seed extract of Decalepis hamiltonii in alloxan induced diabetic Albino rats. Blood glucose levels and body weights of control and diabetic rats were monitored. In the present study activities of liver enzymes such as glucokinase, glucose -6- phosphatase and fructose -1- 6-diphosphatase were also determined. Glibenclamide an antidiabetic oral drug was used as reference in the present investigation. Oral administration of ethanolic seed extract (20 mg/kg body weight) for 21 days resulted in a significant (P<0.05) decline in blood glucose from 264.03±10.07 to 90.16±2.82 mg/dl and significant recovery in body weight of diabetic rats. There was also a significant (P<0.05) reduction in the activities of glucose-6-phosphatase and fructose-1-6-disphosphatase , glutathione levels, lipid peroxidation as oxidative stress γ-Glutamyl transpeptidase, Glutathione S-transferase, aspartate aminotransferase (AST), alanine aminotransferase (ALT), acid and alkaline phosphatases in liver were...
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Posted by admin on Jan 1, 2016 in |
Asparaginase is used to treat acute lymphoblastic leukemia. Acute lymphoblastic leukaemia is seen in both adults and children. It is a most common pediatric cancer, the disease is more common in ages between 2-5 years children. The causative source of acute lymphoblastic leukaemia to be multiple routes, by genetic susceptibility, endogenous exposure or exogenous exposure. L-asparaginase catalyzes the hydrolysis of L-asparagine to L-aspartic acid. L-asparaginase is commonly used as an antineoplastic agent in lymphoblastic leukaemia chemotherapy. We assumed that a structural bioinformatics approach utilizing homology-based molecular modelling and docking approaches. We used sequence data and a homology-based approach to construct a 3D-structural model of asparaginase-like protein from NCBI (GenBank: AAM28434.1). The predicted model was then used to perform molecular docking simulations with natural flavonoid derivatives to assess their ability. The ligand binding residues were found to be similar to the predicted active site...
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Posted by admin on Jan 1, 2016 in |
Malaria, at one time a rural disease, diversified under the pressure of developments into various ecotypes. These ecotypes have been identified as forest malaria, urban malaria, rural malaria, industrial malaria, border malaria and migration malaria; the latter cutting across boundaries of various epidemiological types. In the present study, an attempt has been made to know the seropositivity of Plasmodium falciparum and Pan Malarial species among the febrile patients attending MIMS General Hospital, Nellimarla, Vizianagaram from January 2013 to December 2014. Out of total no. of patient samples(n=1021)collected, 36(3.52%)were seropositive. Out of total no. of seropositive patients(n=36), 20(55.55%) were males, 10(27.77%)were females and 06(16.66%) were children. In the present study, out of total no. of seropositive patients(n=36), 05(13.89%) were positive for Plasmodium falciparum, 19(52.78%) were positive for Pan Malarial species and 12(33.33%) were positive for mixed infection with Plasmodium falciparum and Pan Malarial species. Out of “20” seropositive males, 01(20%) patient was seropositive for Plasmodium falciparum, 14(73.68%) were positive for Pan malarial species and 05(41.67%) were positive for mixed infection...
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Posted by admin on Jan 1, 2016 in |
Eclipta prostrata is a perennial herb, distributed in the tropical and sub- tropical regions of the world. The phytochemical investigation was carried out to determine the possible bioactive components from the methanol extract of Eclipta prostrata leaves by Gas Chromatography and Mass Spectroscopy (GC-MS).100 grams of the powdered sample was subjected to the soxhlet extraction followed by rotary evaporator and investigated using Perkin – Elmer GC-MS while the mass spectra of the compounds found in the leaf extract was matched with the National Institute of Standards and Technology library. The GC-MS analysis revealed the presence of various compounds with peak area like 10-Octadeconic acid, methyl ester (43.85), c-Sitosterol (36.45%), Pentadecanic acid,14methyl,methyl ester (15.09%), Diethyl Phthalate (13.96%), Glycine, N[(3a,5a,12a]-3,12-dihydroxy 24-oxocholan-24-yl]- (13.82%), Ethanol,2-(9,12-octadecadienyloxy),(ZZ) (13.73%), Oleic acid, eicosyl ester (6.92%). Extracts and metabolites from this plant have been known to possess pharmacological properties. Bioactive constituents exhibit antimicrobial activity and reported to have a commendable sense of...
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