Posted by admin on Sep 30, 2015 in |
The Human immunodeficiency virus(HIV)type-1 integrase is one of the most important target of highly active anti retrovirus therapy (HAART), due toits role to incorporate genetic information into the host DNA, so its prevention to its proper function results in very fine therapeutic effect for the treatment of all acquired immune deficiency syndrome(AIDS), extensive research work on integrase inhibitors(INIs) haven’t carried out till present due to complexities in research with integrase and a very few drug are known to inhibit integrase. Dolutegravir is a new 2nd generation Integrase inhibitor (INIs) in a short list of INIs, recently approved by FDA in the list of HAART, so herein we taken Dolutegravir as a reference structure for virtually identification of more/similar efficient drug like leads then Dolutegravir using three different PDB structures (4S3O, 3S3M & 3S3N) of Integrase having in different mutated state from PDB database ‘RCSB’ versus chemical compounds database ‘ZINC’ using Schrodinger and Discovery Studio software. Using molecular constraint search with similarity coefficient ‘Tanimoto’, 1,65,000 ligands were extracted out and...
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Posted by admin on Sep 30, 2015 in |
The present research was focused on the development of an oily suspension for oral administration of omeprazole in Pediatrics. Currently, for the treatment of this population, extemporaneous preparations are used in hospitals with many limitations in their use as bad organoleptic features and loss of drug activity due to improper manipulation, causing the omeprazole to be sensitive to the acidic environment of the stomach, with the consequent loss of activity. The objectives are to achieve a stable formulation of omeprazole in normal storage conditions and to ensure that this formulation maintains the active ingredient protected from the hostile environment of the stomach, which causes degradation of the drug. An oily omeprazole suspension was elaborated and compared to three master formulas usually employed in hospitals (pellets suspension, xanthan suspension, Ora-Sweet®suspension). The omeprazole suspension has been characterized as follows: appearance and organoleptic characteristics, content uniformity test, viscosity, study of dissolution rate and formulation...
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Posted by admin on Sep 30, 2015 in |
Nigella sativa (NS) is a commonly used herb in Asian and African countries. Extensive experimental studies have been done to shed light on its hepatoprotective, immunumodulator, antioxidant, antimicrobial and several other useful pharmacological properties. However, there is dearth of evidence to evaluate the effect of Nigella sativa extract in neuropathic pain. In this study we have used neuropathic pain model of Wistar rats using intraperitoneal cisplatin injection twice weekly for 4.5 weeks. Animals were divided into six groups of six rats each. Ethanolic extract of Nigella sativa, pregabalin, tramadol or combination of pregabalin and tramadol were given orally in Wistar rats in which neuropathy had been induced by cisplatin i.p. injections for 4.5 weeks. Single dose of drugs were given and pain assessment was done by soft touch, crude touch, Eddy’s hotplate and tail flick analgesiometer at 0, 30, 60, 90, 120 and 240 minutes. Nigella sativa showed significant analgesic effect on cisplatin induced neuropathic pain in all the experiments however pregabalin and tramadol showed better...
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Posted by admin on Sep 30, 2015 in |
A simple, rapid and isocratic reversed phase high performance liquid chromatographic (RP-HPLC) stability indicating method was developed and validated for the determination of Imatinib in tablet dosage form. Imatinib drug was exposed to several stress conditions. The proposed method proved to be stability indicating by forced degradation experiments and mass balance study. The chromatographic separation was achieved using Eclipse XDB-C18 (150 mm X 4.6 mm) 5µ analytical column as the stationary phase with isocratic elution of the mobile phase composition of 1.5 g of Sodium dihydrogen phosphate in to 500 ml of water and adjust pH 8.00 with Triethylamine (buffer preparation) : pepared a mixture of 300 ml volumes of methanol and 200 ml volumes of acetonitrile (solvent mixture) mixed in a proportion of 450:550v/v at a flow rate of 1.0 ml/minute. The column oven temperature was maintained at 50°C. The Sample temperature was maintained at room temperature. The injection volume was set to 20 µl and the detector was performed at 265 nm. The retention time of the...
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Posted by admin on Sep 30, 2015 in |
This review covers of the results of previously conducted retrospective and prospective clinical trials and data of Cochrane reviews to examine the effects of D-penicillamine (DPA) for neonatal hyperbilirubinemia and associated low incidence of retinopathy of prematurity (ROP). In the ABO- and Rh-Hemolytic Disease of the Newborn (HDN) DPA significantly reduced the need for both initial and repeated exchange transfusions (ET). In Rh-HDN, almost the half of cases, no ET was performed in the DPA-treated group. Furthermore, this treatment was associated with elimination of all stages of ROP in two trials conducted between 1984 and 1986 in the Department of Pediatrics, Medical University of Debrecen. DPA-therapy of newborn infants may have significant neuroprotective effects in cases jeopardized by bilirubin-induced neurologic dysfunction (BIND) or ROP, which may be related to its capability to alter the nitric oxide (NO) system and to its strong antioxidant effects. It can be assumed that in preventing and treating hyperbilirubinemia, ROP and oxygen toxicity, the mechanism of action of DPA is identical: the protection of...
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