Posted by admin on Feb 29, 2024 in |
The Siddha medicine system, predominantly practiced in South India, encompasses a diverse range of medicinal formulations. Among them, Pancha Moola Kudineer Chooranam (PMKC) from the Therayar Maha Karisal literature is claimed to be effective against ulcers. Helicobacter pylori, causes gastric and duodenal ulcers through various transmission routes. It colonizes the duodenal epithelial cells, leading to gastritis, gastric atrophy, and potentially gastric carcinoma. This study provided valuable insights on the potential anti-ulcer activity of root extracts of the formulation PMKC, which includes Glycyrrhiza glabra, Chukrasia tabularis, Terminalia chebula, Terminalia bellirica, and Phyllanthus emblica against Helicobacter pylori through molecular docking. Eight bioactive compounds were identified which underwent docking calculations. The results indicated that these phytochemicals interacted with the active amino acid residues of H. pylori urease, suggesting potential inhibitory effects on the enzyme. Among the compounds tested, Glycyrrhizin demonstrated the highest inhibition against H. pylori urease, followed by Gallic acid. Maslinic acid and Chuktabrin showed minimal inhibition. In conclusion, the active components of PMKC exhibited significant binding affinity to H. pylori...
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Posted by admin on Jan 31, 2024 in |
Cancer is a complex and life-threatening disease characterized by abnormal cell growth. Several AAA+ ATPases are involved in the oncogenic pathways, however, the role of an ATPase protein i.e. N-ethylmaleimaide-Sensitive Factor (NSF) was unclear. Therefore, we performed systematic bioinformatics analysis to predict the prospect of NSF as a cancer biomarker. NSF is found to be upregulated in some cancers whereas down regulated in others. Additionally, this expression was found to be associated with the reduced survival rate of cancer patients. We investigated NFS’s mutation and copy number amplification status across the cancers since the genetic alteration is a major cause of cancer. Several hotspot mutation sites were observed at the ATPase domain of NSF indicating that these mutations might have a role in carcinogenesis. Additionally, the copy number amplification study revealed a high amplification percentage of NSF across the cancers. We further focused on the functional characteristics of NSF across cancers. Fascinatingly, we found many interacting protein partners of NSF that are key in carcinogenesis. We further focused on the probable oncogenic...
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Posted by admin on Jan 31, 2024 in |
The main aim of the present study is to isolate and identify potent actinobacteria from mangrove sediments of SPSR Nellore district, Andhra Pradesh, India along with their antimicrobial activity. The samples collected were initially pretreated with calcium carbonate followed by dry heating. Dilution plate technique was employed to isolate actinobacteria employing two selective media viz. yeast extract malt extract dextrose agar (YMD) and starch casein agar (SCA) amended with NaCl (4%). The media were supplemented with streptomycin (25μg/mL) and nystatin (25μg/mL) to inhibit the growth of bacteria and fungi. Over all 10 strains (VLKK-1 to VLKK-10) were isolated and screened for antimicrobial activity. The potent strain was identified as Streptomyces carpaticus (Gen bank accession number: OR144182) by polyphasic taxonomy including morphological, cultural, physiological and biochemical characters along with 16S rRNA analysis. The growth pattern of the strain was studied by culturing in YMD broth. The culture broth was extracted with ethyl acetate (1:1) and tested for antimicrobial activity against Pseudomonas aeruginosa, Escherichia coli, Candida albicans and Aspergillus niger. The...
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Posted by admin on Jan 31, 2024 in |
Ramipril is an angiotensin-converting enzyme inhibitor and is used in treating high blood pressure. Hydrochlorothiazide is a diuretic drug that increases urine output. High-Performance Thin-Layer Chromatography is a sophisticated form of thin-layer chromatography which is a separation technique and is used in various bioavailability studies to study marketed formulations as well as novel therapeutic agents. We aimed to develop and validate a simple, precise, accurate, and rapid HPTLC method for the simultaneous estimation of Ramipril and Hydrochlorothiazide in the combined solid dosage form. Precoated silica gel G60F254 was used as the stationary phase. The mobile phase used was a mixture of methanol: toluene: ethyl acetate: glacial acetic acid (1:6:3:0.5 %v/v). Spot detection was carried out at 210 nm. Validation of the method was done in terms of linearity, accuracy, precision, and specificity. A linear calibration curve was obtained between 2000 to 12000 ng/spot for Ramipril and 500 to 3000 ng/spot for Hydrochlorothiazide. The limit of detection and the limit of quantification for the Ramipril was found to be 434.1...
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Posted by admin on Jan 31, 2024 in |
Aim and Objective: The present study was done to assess the prevalence of polypharmacy and to study its consequences in elderly people. Methodology: The study was prospective observational study, done in 500 elderly patients attending the Department of General Medicine in rural tertiary care teaching hospital in Telangana. The study was done for a period of 6 months from April 2019 to September 2019. Descriptive data was expressed in mean and percentages. Chi- square test was done to assess potential drug-drug interactions and number of medications received by the patients (p<0.05 is considered significant). Results: Polypharmacy was present in 70.75% of elderly patients. The mean drugs prescribed for each patient was 5.7%. Severe potential drug-drug interactions were present in 16.82% patients. Potentially inappropriate medications were assessed using Beer’s criteria and were present in 35.52% of patients. Conclusion: This study thus highlights the importance of reducing the practice of polypharmacy by the treating physician to reduce morbidity and to decrease economic burden on the elderly...
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