Posted by admin on Dec 31, 2015 in |
A novel (N’, N”E, N’, N”E)- N’, N” – (2, 2′ – (propane-1,3-diylbis(sulfanediyl))bis(1-(4-chlorophenyl) ethan – 2 – yl – 1 -ylidene))bis(2-hydroxybenzohydrazide)(CAPSH) is identified as a sensitive and selective analytical reagent for the determination of trace levels of Co(II) in environmental samples like in food, water, synthetic samples and medicinal plants by spectrophotometry. CAPSH reacts with cobalt(II) at pH 7.0, in aqueous DMF to form orange colored 1:1 (Co-CAPSH) complex shows maximum absorbance (λmax) at 380nm. The color reaction is immediate and the absorbance remains constant for about 24h. The molar absorptivity coefficient and Sandell’s sensitivity are found to be 7.9×104 L.mol-1 cm-1 and 0.048μg cm-2 of cobalt(II) respectively. The linear regression coefficient is 0.9998 and standard deviation is 0.0050. The system obeys Beer`s law in the range of 00.059-0.59μg mL-1of cobalt(II). Large number of cations, anions and complexing agents do not interfere in this method. This method is successfully applied for the determination of cobalt(II) in environmental samples like food, water (potable and polluted), synthetic samples and medicinal...
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Posted by admin on Dec 31, 2015 in |
Acetylcholinesterase (AChE), a member of α/ß hydrolase protein super family, is the key enzyme in the breakdown of acetylcholine (ACh) – an important neurotransmitter. Inhibition of this enzyme has been considered an auspicious strategy for the treatment of various neurological disorders such as Alzheimer’s disease (AD). In this study, crude methanolic Ipomoea muricata seed extract was subjected for analysis of its potential AChE inhibitory activity using a microplate assay based on Ellman’s method. I. muricata’s inhibitory activity was compared using Donepezil, a prescription drug for AD treatment. Two time intervals were used: 40 minutes and 24 hours. Based on the findings, Donepezil exhibited greater potency (IC50 > 0.005 μg/mL) at both time intervals in contrast to I. muricata (IC50 = 39.67 μg/mL) at 40 minutes and (IC50 > 57.44 μg/mL) at 24 hours. It is important to note that the crude methanolic seed extract of I. muricata has been shown to cause moderate but persistent inhibition of AChE in comparison to Donepezil which has shown a strong but short-lived...
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Posted by admin on Dec 31, 2015 in |
Many microorganisms thrive on the abundant nutrients in the rhizosphere and they produce antagonistic metabolites. The present study is an attempt to investigate the potent bacteria producing antibiotics against pathogenic microorganisms from the rhizosphere soil samples of Kanjamalai hills, Salem district, Tamilnadu, India. A total of 6 isolates of bacteria was obtained and screened for inhibitory activity against selected opportunistic pathogens. Potent bacterial isolate (A6) was identified as Pseudomonas aeruginosa by cultural, morphological and biochemical characterization. The antimicrobial activity of broth culture of Pseudomanas aeruginosa was also observed. The antimicrobial active compound was partially purified from the broth culture and its concentration was estimated to be 373.086 µg/ml. The obtained protein was separated in SDS-PAGE and its molecular weight was determined as 66.4 kD, 44.3 kD, 29.0 kD, 20.1 kD and 14.3 kD using standard molecular markers. The results showed that the isolated strain has high potential against Bacillus megaterium and Aspergillus niger. It can be further investigated to identify the antibacterial compounds produced and their mechanisms of...
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Posted by admin on Dec 31, 2015 in |
A pessary is a type of suppository that is meant to be inserted into the vagina for treatment of local or systemic diseases, in folk medicine garlic bulb is inserted into vagina for treatment of candidiasis. The aim of this work is to formulate and evaluate aqueous extract of garlic as a pessary. Aqueous garlic (Allium sativum) extract was obtained from the garlic bulbs. The pessary was formulated using the pouring method and cocoa butter as a base. The formulated pessaries were evaluated using physical appearance, crushing strength test, disintegration and dissolution times tests as well as content uniformity. The visual examination showed brilliant and smooth surfaced, consistently bullet shaped, light brown colour and mild garlic odour. The results of crushing strength (3.96 ± 0.92 KgF), disintegration time (5.17 ± 0.17 min) and content uniformity (96.03 ± 7.26 %) were all within the normal limits. The result obtained from the dissolution time test showed that the pessary dissolved within 30 min. The results indicated that aqueous garlic extract can...
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Posted by admin on Dec 1, 2015 in |
In Advances drug design technologies, a large no of drug molecules are being introduced in every year but many of these molecules have problems like their solubility, stability, bioavailability and its long lasting side effects. Low bioavailability is one of the serious but curable problems in case of the drug molecule. Low bioavailability happens because a lot of molecules unable to permeation the gastrointestinal epithelia. There are some other factors also which responsible for low bioavailability i.e. low lipophilicity and zwitterionic character at physiological pH, poor water solubility or efflux by P-glycoprotein (P-gp) etc. The object of this review is to explore the concept of bioavailability to achieve better therapeutic response in appropriate dose using natural drugs and natural products like ginger, caraway, aloe, quercetin, glycyrrhizin piperine, curcumin etc. The use of natural products is the most reliable means for bioavailability enhancement because these are safe, non-toxic, economical, easily procured, non-addictive, pharmacologically inert and non-allergenic in nature etc. This review explores the natural drugs from plant and animal sources...
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