Posted by admin on Nov 30, 2015 in |
Lawsonia inermis L. (Lawsone) is reported to contain carbohydrates, proteins, flavonoids and phenolic compounds, alkaloids, terpenoids, coumarins, xanthones and fatty acids. The plant has also been reported to have hepatoprotective, anti-inflammatory, antibacterial, antifungal, antiviral, antioxidant and anticancer properties. Lawsone has low bioavailability because it is less soluble in water and it is rapidly eliminated from body. The aim of this study was to prepare the phytosome of lawsone and evaluate it. Different phytosome complexes of lawsone containing molar ratio of 1:1, 1:2, 2:1 and 2:2 of lawsone and soya lecithin were prepared by the antisolvent precipitation technique. The phytosome was characterized by SEM, DSC and FTIR. Antifungal activity of phytosome of lawsone was evaluated on Candida albicans (NCIM 3471) fungi by using ketoconazole as standard drug. The in-vitro permeation study was done on rat skin. The anti-inflammatory activity was evaluated in male wistar rats. SEM and DSC data showed that phytosome complex of lawsone has irregular size vesicles consisting of soya lecithin and lawsone was found to be intercalated...
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Posted by admin on Nov 30, 2015 in |
Sugarcane is the major cash crop of the Indian tropical and sub-tropical region. Mostly the foremost part of Indian economy is based on sugarcane crop, which is cultivated major state in India. Phyto-components are the compound that occurred in plant naturally and play important role for biologically activity (Anti fungal, Anti cancer Anti diabetic etc), to prevent the many disease, scavenging and chelating the free radicals. In the present study we identified the phytocomponent presence in juice sample of Mungo 254 (Saccharum barberi) through GC-MS analysis. The major compound sucrose (30.64%) with retention time 12.18 and lower percentage compound was Pentanal, 2-methyl (0.10%) with 6.48 retention time. The identified compounds having biological and pharmacological activity such as Antimicrobial, Antifungal, Anticancer, Antioxidant, Antimutagenic and Hypercholesterolemic...
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Posted by admin on Nov 30, 2015 in |
High performance thin layer chromatographic method has been developed for the determination of Olopatadine (OLO) and Montelukast (MONT) in their combined dosage form. Merck HPTLC aluminum plates of silica gel G60 F254, (10 × 10 cm) was used for separation of combined with 250 μm thickness using Chloroform: Ethyl acetate: Methanol: Triethylamine (6: 4.5: 2.5: 0.8, v/v/v/v) as mobile phase. HPTLC separation of the both drugs were carried out and followed by densitometric measurement was performed in the absorbance mode at 254 nm. The drugs were resolved satisfactorily with Rf values of 0.39 ± 0.01 and 0.66 ± 0.01 for OLO and MONT, respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification and robustness. The developed HPTLC method can be applied for identification and quantitative determination of OLO and MONT in bulk drug and drug...
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Posted by admin on Nov 30, 2015 in |
A simple, precise, and robust spectrophotometric method for the determination of Cefotaxime sodium has been investigated and validated. The proposed chemical reaction involves the reaction of primary amine of Cefotaxime sodium with nitrite solution in acidic medium to form a diazotized product, followed by the reaction of the diazotized product with suitable coupling reagent resulting in a colored product. Cefotaxime sodium has shown λmax 500nm. The method was found linear obeying Beer Lambert law at concentration range of 1-20 ppm. In the method detection limits was observed 0.744 ppm with quantification limit of 2.48 ppm and standard deviation of 0.248. In the present work an attempt was made to develop a simple, less expensive and more reliable method for the determination of Cefotaxime sodium in pure and pharmaceutical...
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Posted by admin on Nov 30, 2015 in |
Natural products derived from plants and herbs have always had an important role in drug development. Especially in case of cancer, there is a huge demand for effective chemopreventive and chemotherapeutic agents with natural base and low toxic effects on normal non-cancerous cells. In this work, we have studied the effects of Pistacia atlantica sub kurdica (Baneh) extract on microtubule proteins and their biochemical features. Since microtubules are directly involved in spindle formation and cell division, they can be a very good target for anticancer drugs. We hypothesized that Baneh extract has the potential to inhibit microtubule polymerization and dynamics and as a result inhibit spindle formation during cell division. In order to analyze the effect of Baneh extract on microtubule proteins we decided to investigate these proteins biochemical characteristics in the presence of different concentrations of the extract. We used cell culture model to confirm cytotoxicity of Baneh extract. We then did some polymerization kinetics and dynamics study on microtubule proteins in the presence of various concentrations of...
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