Posted by admin on Jan 31, 2016 in |
An accurate, precise and rapid RP-HPLC method was developed and subsequently validated for the determination of Empagliflozin in API. Better separation of the drug was achieved on Intersil column(150x40mm, 5 μm) with the mobile phase consisted of mixture of 0.01 M acetate buffer, methanol in ratio of (30:70v/v ) at flow rate of 2ml/min, with detection at 260nm using PDA detector. The retention time was found to be 1.223min. The method was found to be linear in the range of 2-150ug/ml with a correlation coefficient (r2) of 0.999. The LOD and LOQ of the method were calculated to be 0.7 and 1.91μg/ml respectively. The Precision was estimated by employing repeatability; intra-day and inter-day studies and the results were calculated as %RSD values and were found to be within the limits. Recovery of Empagliflozin was found to be in the range of 99.7-100-% which confirms the accuracy of the method. The proposed HPLC method is validated using standard ICH...
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Posted by admin on Jan 31, 2016 in |
Betulin, a known pentacyclic tri-terpenoid, possessing diverse pharmacological properties was determined using HPLC in stem bark methanol extract of Tectona grandis Linn. distributed in greater part of India. Furthermore, Tectona grandis Linn. is used traditionally in treatment of bronchitis, dysentery, headache, leukoderma, leprosy and constipation. The present study was aimed to validate HPLC method for the determination of betulin in Tectona grandis Linn. (bark). The chromatographic separation was achieved using Waters HPLC instrument, reverse phase Hypersil C18 column (250 mm) under isocratic elution of acetonitrile-water 85:15 (v/v) with a flow rate of 1.0mL min ˉ¹ and the run time was set at 15 min. The detection was done at 210 nm and the column temperature was maintained at 25⁰ C. The method was linear for betulin over the concentration range of 28.42- 53.99 ppm (R² = 0.9974) in Tectona grandis Linn. (bark). The quantification of betulin in methanol extract of Tectona grandis Linn. was validated in terms of system precision, method precision, linearity, recovery, robustness, ruggedness, LOD and LOQ...
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Posted by admin on Jan 31, 2016 in |
Various parts of the plant Butea monosperma have been used traditionally for many of the diseases like anti-inflammatory, antimicrobial, anthelmintic, antidiabetic, diuretic, analgesic, antitumor, anticancer, astringent activities. Among the various parts of the Butea monosperma first choice is selection of leaves in our current research work because only scanty work has been done on leaves. So it is an attempt to carryout phytochemical evaluation of various leaf extracts and to isolate active constituents and study the characterization of isolated compounds by FTIR, NMR and Mass spectroscopy. The isolated components evaluated for the pharmacological activities along with antimicrobial studies. The pharmacological studies of Petroleum ether, hexane, Chloroform, Ethyl acetate and Ethanol extracts of Butea Monosperma leaves shows the anti-inflammatory activity and anthelmintic activity. The petroleum ether and chloroform extract of Butea Monosperma leaves shows antioxidant activity. The Petroleum ether, Hexane, Chloroform, Ethyl acetate and Ethanol extracts of Butea Monosperma leaves have no anti bacterial and antifungal...
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Posted by admin on Jan 31, 2016 in |
The healing of an adult skin wound is a complex process of different tissues and cell lineages. Understanding wound healing involves understanding of inflammation, proliferation, and maturation phase. The present study was aimed at evaluation of proliferative action of hydroalcoholic extract of Trigonella foenum graecum (HETG) in albino rats using excision and incision wound model. HETG 5% W/W and HETG 10% W/W in simple ointment base were used and Povidone iodine 5%w/w was used as standard. The experimental animals were topically applied with test and standard twice daily for consecutive 14 days. Proliferative activity was studied in excision wound model and incision wound model. The parameters studied are rate of wound contraction and period of epithelisation in excision wound model. Tensile strength and hydroxyproline content in the scab was studied in incision wound model. Histopathological studies were performed. Based on the results HETG 10% W/W shown significant cell proliferative activity in granulation, contraction and epithelialisation of proliferation...
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Posted by admin on Jan 31, 2016 in |
Bacterial diseases are highly virulent and cause significant crop loss in Anthereae mylittadrury (Daba TV).Therefore an attempt has been made to evaluate the efficient concentration of sodium hypochlorite in controlling bacterial infection through studies on mortality, Total haemocytes, Protein carbonyl content, Alanine transaminase, Aspartate transaminase in the fifth in star larvae. Results reveal that supplementation of 0.05% sodium hypochlorite concentration had significantly reduced the mortality rate due to bacterial infections. In comparison with the control (14788±123), the haemocyte count in 0.05% sodium hypochlorite supplemented larvae were recorded low as14125±146 whereas 0.1% and 0.01%concentration supplementation resulted in an increase of 15025±158 and 14965±185 haemocytes. In comparison with the control results also show that protein carbonyl content was doubled with 0.05% supplementation whereas an increase of 33.3% and 16.6% was recorded with 0.01% and 0.1% respectively. Alanine aminotransferase had shown significant increase of 138.7% with 0.05% sodium hypochlorite supplementation followed by 39% increase with 0.01% concentration in its activity. Supplementation with 0.1% did not show much variation in Alanine aminotransferase from...
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