Posted by admin on Sep 30, 2015 in |
Oxidative stress contributes towards initiation and progression of hepatic damage in a variety of liver disorders (e.g. Hepatitis C, Hepatitis B, non alcoholic fatty liver disease amongst others). Hence, there is a great demand for the development of agents with potent antioxidant effect. There are various plant derived products which act as antioxidants. The aim of the present investigation is to evaluate the efficacy of Brassica oleracea extract as a hepatoprotective antioxidant. Brassica genus encompassing broccoli, cabbage, cauliflower and radish, have received much attention, due to their glucosinolate content. Glucosinolates are hydrolysed by the endogenous enzyme myrosinase and their degradation products are reported to have anticancer activity. The FRAP assay (ferric reducing ability of plasma) is a simple test that has been employed in this study to detect the free radical scavenging activity of Brassica oleracea extract. The present paper discusses the antioxidant and antibacterial activity of Brassica oleracea...
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Posted by admin on Sep 30, 2015 in |
The purpose of this research was to develop a drug-in-adhesive type transdermal drug delivery system containing drug, buflomedil hydrochloride, pressure sensitive adhesive (PSA), Duro-Tak 387-2052 by the solvent evaporation technique. Different concentrations of penetration enhancers; oleic acid and isopropyl myristate (IPM) were used to enhance the transdermal permeation of buflomedil hydrochloride. 3M Scotchpak TM 9723 polyester film was used as a backing membrane and 3M Scotchpak TM 1022 – Fluoropolymer Coated Polyester film was used as a release liner for the preparation of transdermal patches. FTIR and DSC studies were done to know any possible interaction between drug and polymer. Prepared drug-in-adhesive transdermal patches were physically evaluated with regards to thickness, weight variation, surface pH, folding endurance, moisture content and moisture uptake. The adhesion properties of the patches were very satisfactory. Also, the prepared patches showed good uniformity with regard to drug content. In vitro drug permeation studies of formulations were performed by using Franz diffusion cell through dialysis membrane. It was observed that the formulation containing 95% adhesive...
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Posted by admin on Sep 30, 2015 in |
Aim: Hypothyroidism is one of the commonest metabolic diseases which afflicts females more than males and is often associated with myopathy. Reports on relationship of muscle-markers with thyroid hormones in the (a) euthyroid population in general and (b) hypothyroid population in India are scarce. Materials and Methods: A randomized case-control study on the female population for which 60 hypothyroids and 30 euthyroid controls were selected. Total triiodothyronine (T3), thyroxine (T4), thyroid stimulating hormone (TSH) and two muscle markers – lactate dehydrogenase (LDH) and creatine kinase (CK) were measured from serum samples using automated analyzers. Results: CK was 71% higher and LDH 23.5% higher in hypothyroids than in euthyroid controls. CK correlated well with T4 in hypothyroids (r= – 0.68, p<0.0001) and T3 in euthyroids (r= 0.62, p= 0.003) but to a lesser extent with others viz. T3 in hypothyroids (r= -0.46, p= 0.0002), T4 in euthyroids (r= -0.49, p= 0.007) and TSH both in hypothyroids and euthyroids (r= 0.43, p= 0.0006; r= -0.40, p= 0.03 respectively). LDH correlated weakly...
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Posted by admin on Sep 30, 2015 in |
Cadmium is a potent neurotoxic heavy metal, which induces oxidative stress and membrane disturbances in brain. Oxidative stress has been implicated in the pathology of number of neurodegenerative disorders including Alzeimers disease. The present study was aimed to evaluate the antioxidant effect of hexane extract of Citrullus vulgaris seed (CVSO) in the brain of cadmium (cd) treated mice as an animal model of oxidative stress. The neuroprotective efficacy of CVSO against Cd was assessed by estimating the levels of lipid peroxidation, non-enzymatic antioxidant, enzymatic antioxidant status and acetylcholinesterase (AChE) activity in mice. CVSO was administered along with Cd for four weeks. The results showed that mice intoxicated with cadmium (5 mg/kg/day) for 4 weeks significantly (p<0.05) reduced the AChE levels in brain, elevated the levels of thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides along with the significant (p<0.05) decrease in the levels of non-enzymatic antioxidants (GSH), enzymatic antioxidants superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) in the brain tissue. In conclusion, CVSO ameliorated cadmium induced brain damage by...
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Posted by admin on Sep 30, 2015 in |
The Human immunodeficiency virus(HIV)type-1 integrase is one of the most important target of highly active anti retrovirus therapy (HAART), due toits role to incorporate genetic information into the host DNA, so its prevention to its proper function results in very fine therapeutic effect for the treatment of all acquired immune deficiency syndrome(AIDS), extensive research work on integrase inhibitors(INIs) haven’t carried out till present due to complexities in research with integrase and a very few drug are known to inhibit integrase. Dolutegravir is a new 2nd generation Integrase inhibitor (INIs) in a short list of INIs, recently approved by FDA in the list of HAART, so herein we taken Dolutegravir as a reference structure for virtually identification of more/similar efficient drug like leads then Dolutegravir using three different PDB structures (4S3O, 3S3M & 3S3N) of Integrase having in different mutated state from PDB database ‘RCSB’ versus chemical compounds database ‘ZINC’ using Schrodinger and Discovery Studio software. Using molecular constraint search with similarity coefficient ‘Tanimoto’, 1,65,000 ligands were extracted out and...
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