Posted by admin on Nov 30, 2015 in |
Inflammation is one of the unique mechanism that help body to protect itself against burn, infection, allergens, toxic chemicals, or other noxious stimuli. It is a defense reaction of the body in order to eliminate or reduce the spread of injurious agent. However, over reaction of the body may be harmful or undesirable. This has resulted in extensive development of anti-inflammatory drugs. Despite the benefits that the drugs hold, it also carries the side effects such as gastric lesions, allergy reactions, tolerance and dependence, as well as resistance. Hence, researchers are working towards ideal medicines of anti-inflammatory with highest efficacy, best potency and lowest or none side effects. This research study evaluates the anti-inflammatory activity of trans-cinnamaldehyde and piperine combination. Preclinical testing of cinnamaldehyde and piperine was done earlier individually on inflammatory models but the combination was not validated for the treatment of inflammation. Therefore, the present study was carried out to evaluate the anti-inflammatory potential of the trans-cinnamaldehyde and piperine combination using carrageenan-induced paw edema model. The combination...
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Posted by admin on Nov 30, 2015 in |
Candesartan cilexetil, a prodrug is a racemic mixture containing one chiral center at the cyclohexyloxy-carbonyloxy- ethyl ester group. It is soluble in dimethyl formamide, acetone, methanol, 0.1 N sodium hydroxide solution and insoluble in water. Objective of the present study is to develop a simple, sensitive, accurate, precise and rapid derivative spectrophotometric method for the estimation of candesartan in pure form. For the estimation of candesartan, solvent system employed was absolute methanol and wavelength of detection (λdet) was 294.3 nm for third order derivative spectroscopy. The linearity was obtained in the range 180 – 280 µg/ml. The regression equation was found to be y = 0.0001x + 0.003 & regression coefficient, R2 was found to be 0.999. The limit of detection is 6.7µg/ml and limit of quantification was fund to be 20.4 µg/ml. Obtained results showed that there is minimum intraday and inter day variation. The developed method was validated and recovery studies were also carried out. Sample recovery using the above method was in good agreement with their...
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Posted by admin on Nov 30, 2015 in |
High performance liquid chromatographic (HPLC) method was described for determination of Pseudoephedrine Hydrochloride (PSE) and Fexofenadine Hydrochloride (FEX). The chromatographic separation was achieved on Kinetex C18, (250 x 4.6 mm i.d), Particle size 5µm. The mobile phase contains a mixture of 0.005 M Dioctyl Sodium Sulfosuccinate Buffer in a mixture of 50 volume of Methanol, 10 volume of Acetonitrile, 40 volume of Water and 1 volume of Glacial Acetic Acid was investigated to separate the drugs from their stressed degradation products. The flow rate was 1.2 ml/min, injection volume of 50 µL, run time of 15 minutes, at column oven temp 50°C. The detector wavelength was 258 nm. PSE and FEX were subjected to stress degradation conditions of hydrolysis (acid and base), oxidation and thermal degradation. Stressed samples were analyzed by the developed procedures. The described method shows excellent linearity over a range of 72 to 720μg/ml and 48 to 480μg/ml PSE and FEX, respectively. Degradation of PSE was observed in oxidative condition but found to be stable in...
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Posted by admin on Nov 30, 2015 in |
Magnetic nanocarriers have been used for specific drug delivery to release therapeutic drugs into target cancer cells. We used bifunctional methotrexate (MTX) conjugated magnetic nanoparticles (MNPs) (~37 nm) engineered by dopamine–polyethylene glycol to targeted folate receptor (FR)-positive cancer cells. For this purpose, MTX was chemically loaded on to MNPs with N, N-dicyclohexylcarbodiimide (DCC) in the absence of NHS at 0°C. Activation process of MTX was analyzed via synthesis of different MTX methyl esters through HPLC instrument. The HPLC analysis showed that in a selective condition and in the presence of 1.1 equiv. of reactiveagent,a-carboxyl group of MTX was more active. Enzymatic release study of MTX demonstrated that the highest rate of drug release was at pH=3.8 with protease enzyme (85.12%). Also, cytotoxicity assay revealed that Fe3O4-DPA-PEG-MTX NPs were able to target over expressed FR cell line (MCF-7) but did not have any effect on FR-negative A549 cells and significantly inhibit...
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Posted by admin on Nov 30, 2015 in |
Simple, sensitive, accurate and precise spectrophotometric methods for quantitative determination of five drugs viz., Tramadol Hydrochloride (TDH) , Dobutamine (DOB), Trimetazidine (TRMZ), Terazocin (TRZ) and Esmolol (ESM) were developed. The method for each drug depends upon oxidation of drugs by Ce (IV) (Excess) and estimating the amount of un reacted Ce (IV) by Rhodamine-B dye at 557 nm. The calibration curves obeyed Beer’s law over the concentration range of 14-130μg ml-1 (TDH), 1-14 μg ml-1 (DOB), 8-120μg ml-1 (TRMZ), 16-130 μg ml-1 (TRZ), and 12-84 μg ml-1 (ESM). The methods have been validated in terms of guidelines of ICH and has been applied to the analysis of...
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