Posted by admin on Dec 1, 2012 in |
Background: Enzyme catalysis is intermediate between homogeneous and heterogeneous catalysis. In general, it is similar to inorganic heterogeneous catalysis and sometimes it is called micro heterogeneous catalysis. Efficient enzyme catalysis depends on high specific nature of enzyme, pH, temperature, optimum temperature, colloidal nature, activator or co-enzyme, inhibitors or poisons. Immobilized β-galatosidase show high activity towards both pure lactose and lactose in skim milk, and a better thermal stability also. Methods: This study was done in CDRI, Lucknow. In this study β-galatosidase was extracted by Kluyveromyces lactis and enzyme assay done by GOD/POD method. Partial purification of enzyme was done by precipitation with ammonium sulphate, DEAE-cellulose column chromatography followed by SDS-PAGE. Immobilization of K. lactis cells was done by Entrapment method in which we agarose beads, plyacrylamide beads and alginate beads were used to analyze the catalytic activity of β-galatosidase on different parameters. Results: In this study the effect of temperature, pH, and amino acids on normal β-galatosidase and on immobilized β-galatosidase were analyzed. Immobilized enzyme showed the decreasing conversion level...
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Posted by admin on Dec 1, 2012 in |
This study was undertaken to determine the prevalence and determinants of family self-medication in children in northeast India. A validated structured interview schedule was used in 487 caregivers of children attending the out-patient department and in-patient ward of the department of pediatrics, Regional Institute of Medical Sciences, Imphal, India. The study schedule included questions related to the socio-demographic profile of the participants and their practice of self-medication for their children for the last 6 months. Descriptive statistics like mean and percentage were used. Chi square test was used for significance. A p value of <0.05 was considered as significant. The prevalence of self-medication was found to be 56.1%. The preference of self-medication was significantly dependent on the age of children. The preference was, however, not significantly dependent on gender, birth order of children, or educational and socio-economic status of...
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Posted by admin on Dec 1, 2012 in |
Inflammatory diseases including different types of rheumatic diseases are a major and worldwide problem. Now a day’s, world population moves towards herbal remedies for treatment. Several edible plants parts are used in traditional medicine for the treatment of inflammatory conditions. Amomum subulatum Roxb (Zingiberaceae), commonly known as large cardamom, is traditionally used for several medicinal purpose in India. The present study assessed anti-inflammatory activity of its fruits extracts (methanolic and aqueous) at a dose of 250 mg/kg and 500 mg/kg against carrageenan induced paw edema in rats. Both the extracts were able to show a dose dependent anti-inflammatory activity with compared to ibuprofen as a...
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Posted by admin on Dec 1, 2012 in |
Due to the limited life span of platelets during regular storage in blood banks, we were interested to investigate the both potential hemostatic effect and dose-dependent response properties of non-viable platelet preparations with injection of two high and low doses in experimental animal model. Infusible platelet membrane (IPM) was prepared from outdated platelet concentrates. Platelet concentrates were pooled, disrupted by freeze-thaw procedure, pasteurized for 20 hours to inactivate possible viral or bacterial contaminants and finally formulated with sucrose and human serum albumin. Rabbits were made thrombocytopenic. Administration of IPM at two doses of 0.5 and 2.0 mg per kg results significant reduction in the bleeding time. The values of bleeding time in the 80 data sets were obtained. Reduction in the percentage of bleeding time elevation during 2, 4, 6 and 24 hours after injections of 0.5 mg/kg were found 56.8, 66.0, 73.7 ,96.8 and after 2.0 mg/kg injections were observed 24.8, 39.0, 52.4 and 95.6 respectively. We concluded that IPM can significantly reduce bleeding time in thrombocytopenic rabbits...
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Posted by admin on Dec 1, 2012 in |
Staphylococcus aureus (S. aureus) is one of the most serious gram-positive bacteria causing several infections. Because of its intrinsic ability to develop resistance to many antibiotics, nowadays only few drugs can be confidently mentioned for the treatment of S. aureus infections. The study aimed to investigate the antibiotics susceptibility pattern of S. aureus among patients. A cross-sectional study was conducted antibiotic susceptibility pattern of S. aureus among patients. A total of 323 wound and nasal swab specimens were collected and inoculated on mannitol salt agar (Oxoid) and incubated at 37oC for 18 to 24 hours. For primary cultures with bacterial growth, Gram-staining and specific biochemical tests (catalase and coagulase) were used to identify the study organism. Sensitivity of the isolates to ten commonly used antibiotics was determined by modified Kirby-Bauer antibiotic sensitivity testing method. The isolates were found to be highly resistant to penicillin G and cephalothin (98.8%; each). Chloramphenicol, amoxicillin/clavulanic acid and oxacillin were also resisted by 53.1%, 50.6% and 38.3% of the isolates, respectively. Least resistance was...
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