Posted by admin on Nov 30, 2019 in |
In this manuscript, we designed and synthesized 1,4-naphthoquinone derivatives. These derivatives were characterized using the different analytical techniques such as 1H NMR, 13C NMR, FTIR, Mass spectroscopy, melting point, and elemental analysis. The synthesized compounds (MB (1-10)) further subjected to assess the anticancer activity using different cancer cell lines such as MCF-7, HeLa, and HepG2. The compound MB-10 was observed to be the most active against these three cancer cell lines i.e. MCF-7 (IC50 = 16.13± 0.29 µM), HeLa (IC50 = 12.98 ± 0.37 µM) and HepG2 (IC50 = 18.73 ± 0.65 µM). Compound MB10 has also shown potent tyrosine kinase inhibitory activity with IC50 = 1.23 ± 0.12 µM. Moreover, molecular docking investigation revealed that compound MB-10 has strong binding affinity to the amino residue of the active site of enzyme protein tyrosine kinase, which contingent the cytotoxic activities. These outcomes give promising beginning stages to assist in the improvement of cancer as novel and powerful anticancer...
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Posted by admin on Nov 30, 2019 in |
A simple, efficient HPLC method has been developed and validated for simultaneous determination of Amantadine hydrochloride and Oseltamivir phosphate using pre-column derivatization with phenyl isothiocyanate (PITC). The chromatography separation was achieved on Phenomenex C18 column of 250 × 4.6 mm 5 µm particle size, mobile phase used for chromatographic run consisted of acetonitrile and water (60: 40% v/v) with a flow rate of 2 mL/min. The analysis was performed at ambient temperature, and the eluent was monitored at 250 nm using a UV detector. The retention time of Amantadine hydrochloride and Oseltamivir phosphate was found to be 7.1 min and 3.2 min. The limit of detection (LOD) for Amantadine hydrochloride and Oseltamivir phosphate was observed to be 0.1µg/mL and 0.25 µg/mL, respectively. Limit of quantification (LOQ) was found to be 0.5 µg/mL and 0.25 µg/mL. The developed method was validated as per ICH guidelines using parameters like linearity, specificity, precision, linearity, accuracy, ruggedness, robustness, LOD, and LOQ. All the validation parameters were found to be well within the acceptance...
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Posted by admin on Nov 30, 2019 in |
Antibiotic is the powerful invention of science to damage bacterial infections. But nowadays, the use of careless increasing doses of this antibiotic has led to increasing the resistant power of enemy bacteria. Bacteria are caused by many infectious diseases by proliferation within quorum sensing (QS) mediated biofilms. Quorum sensing can develop microbial pathogenic traits, including biofilm formation. Try to smash biofilms have led the identification of bioactive molecules evolved by prokaryotes and eukaryotes. The activity of these bioactive molecules is done by quenching the QS systems. For this reason, we need to inhibit quorum sensing, which may be done by various types of inhibitors called quorum sensing inhibitors (QSI). Synthetic and natural QSIs plays a vital role to inhibit QS. In this review, some examples of QS inhibitors like synthetic and natural based QS inhibitors are highlighted. This review is also focused on the inhibition of QS mechanisms and the application of QSI in various...
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Posted by admin on Nov 30, 2019 in |
Schizophrenia is a chronic and severe mental disorder that affects how a person thinks, feels, and behaves. People with schizophrenia may seem like they have lost touch with reality. Schizophrenia is a split personality disorder which is related to mental illness. Generally, schizophrenia affects approximately 1 percent of all adults’ population. Schizophrenia is different types such as paranoid in which auditory hallucinations occur, some time disorganized schizophrenia occurs in which disorganized speech and behavior are shown. The hypothesis of schizophrenia included dopamine hypothesis, glutamatergic hypothesis, GABAergic hypothesis. Pharmacologically, they are characterized as dopamine receptor antagonists, though many of them also act on other targets, particularly 5-hydroxytryptamine (5-HT) receptors. The antipsychotic agents are used for the treatment of schizophrenia. Based on pharmacologic action, the chemical structures of antipsychotic agents contain reserpine- like compounds, phenothiazines derivatives and compounds include various tricyclic derivatives with a central 7-membered ring to which methyl piperazine is linked. The activity of this group is widely scattered, containing among the weakest and more potent of the apomorphine...
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Posted by admin on Nov 30, 2019 in |
Inflammation is the vascular event shown by the body against the external material by activating different cells and releasing various chemical mediators to prevent noxious effects. Many drugs like steroidal and non-steroidal drugs are available till the date to treat inflammation but have a number of side effects and centralized on to counteract the COX and LOX pathways. This perspective will use to search for new drugs that can work to target other pro-inflammatory mediators involve in inflammation and act as target for the inhibition of inflammatory processes and minimize the side effects. The therapeutic targets are any proteins, receptors, pro-inflammatory mediators or enzymes that can be involved in the inflammatory reaction. The targeted therapy acts on it and inhibits targets to combat the inflammation. The recent study assembles the data on novel targets and future anti-inflammatory drugs and the best treatment for incurable inflammatory...
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