Posted by admin on May 31, 2019 in |
A FTIR method was developed to estimate Artemether and Lumefantrine simultaneously in bulk as well as in formulations. It is based on the measurement of absorption of radiation at the absorption band of ether at 1113.10-1091.77 cm-1 for ART and phenyl substitution band at 893.65-857.08 cm-1 for LUM, because those absorption bands did not occur in excipients present in a pharmaceutical preparation. The proposed method was validated as per ICH guidelines. The calibration curve was obtained for a series of concentration in the range of 17-470 mg for ART and 10-250 mg for LUM, and it was found to be linear. The linear regression equation was y = +84.78-193.1*x for ART and y = +353.75+2129.1*x for LUM with correlation coefficient value 0.999 for ART and LUM which were within the acceptance criteria. The precision was measured regarding repeatability and % RSD was calculated and was found to be 0.831 for intraday and 0.831 for inter-day precision. Recovery was carried out standard addition method at three different levels which are...
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Posted by admin on May 31, 2019 in |
The current research was accomplished to assess the analgesic activity of Argyreia cymosa (A. cymosa) methanolic extracts (MEAC) at 200 and 400 mg/kg. The entire herb was extracted with the methanol (MEAC). Acute oral toxicity of the extract was according to OECD-423 recommendations. Analgesic activity was explored by utilizing hot plate, tail immersion and acetic acid-evoked writhing models. In acute toxicity study, no mortality was observed when each extract was orally administered with 2000 mg/kg. At the doses (200 and 400 mg/kg) methanolic extract of Argyreia cymosa demonstrated considerable and dosed reliant analgesic effect. The conclusions from the present investigation confirmed the folkloric usage of Argyreia cymosa as an analgesic agent. This significant analgesic impact might be because of the presence of steroids, flavonoids, and glycosides within the extract. But, further phytochemical, as well as biological tests, are needed to determine the additional active chemical constituents responsible for the antinociceptive...
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Posted by admin on May 31, 2019 in |
Quality of life is an essential measure that proves the effectiveness of health care, health level, and well-being. Kidney diseases are one of the major health problems that affect Quality of life. This study aimed to assess the quality of life in dialysis (CRF-D) and non-dialysis (CRF-ND) chronic renal failure (CRF) patients using Kidney Disease Quality of Life-short form (KDQOL-SFTM) and identify the differences in disease symptoms between these two groups. 200 CRF patients were included in the study. Our findings showed that some symptom burden was higher in the non-dialysis group compared to the dialysis group, but most of the symptoms did not reach statistical significance. Similarly, our results indicate no differences in quality of life and symptoms between CRF patients with dialysis and without...
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Posted by admin on May 31, 2019 in |
Plasmodium knowlesi, a simian malaria parasite, is widely used as a malaria model and now characterized as a clinically significant parasite that can lead to outbreaks throughout most countries in Southeast Asia. Despite its importance, both fundamental and clinical studies of P. knowlesi have been impeded by the lack of a convenient in-vitro culture system partly due to the parasite’s preference for reticulocytes. Due to a limited number of reticulocytes that could be collected from fresh sources such as umbilical cord, bone marrow, and peripheral blood, scientists have opted reticulocytes generated from CD34+ hematopoietic stem cells (HSCs) that have been cultured in an in-vitro system. With sufficient growth factors (GFs) and differentiation factors (DFs), a higher number and more homogenous populations of reticulocytes could be obtained from HSCs in-vitro. Here, we review an approach to produce massive expansion of CD34+ HSCs and their differentiation into reticulocytes that could be used for the establishment of a continuous in-vitro culture of P. knowlesi or P. vivax. The successful development of the...
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Posted by admin on May 31, 2019 in |
Adenocalymma alliaceum is an important medicinal plant belongs to the family Bignoniaceae. The antibacterial and phytochemical studies on the leaves of this plant were carried out by using the disc diffusion method and Thin Layer Chromatography. The methanolic leaf extract showed a brown colored band on TLC plate with an Rf value 0.25 represents the presence of sulfide compound. The extract was tested against four bacterial strains, namely Enterobacter aerogenes, Salmonella enterica, Vibrio cholerae (gram positive) and Streptococcus haemolyticus (gram negative). The activity was determined by measuring the diameter of zone of inhibition in mm. The result obtained from the present study indicates that the methanolic leaf extract was active only against gram-positive bacteria. The maximum zone of inhibition was recorded in Salmonella enterica (12...
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