Posted by admin on Nov 30, 2020 in |
Introduction: Inhaled Corticosteroids remain the mainstay therapy for asthma. Uncontrolled asthma (UA) dose not respond to the highest inhaled corticosteroids, which remains a challenge for clinicians. Multidrug resistance-associated protein 1 (MRP-1) are molecular “pumps,” which expel toxins and modulate corticosteroids’ pharmacokinetics and may lead to UA. Moreover, their role in UA is still not clear and needs further investigation. Our study evaluated the expression and functionality of MRP-1 in patients with controlled asthma (CA) and UA. Materials and Methods: UA was defined as a lack of control with high dose inhaled corticosteroids along with the use of other controller medications. Whole blood MRP-1 expression and functional activity on peripheral blood mononuclear cells (PBMCs) in UA (n=38), (mean age = 53.53 ± 11.85, male = 18) patients, CA) (n=60) (mean age = 51.65 ± 16.99, male = 31). MRP-1 was analyzed by flow cytometry. Result: Expression of MRP-1 positivity on lymphocyte gated population was significantly higher in UA than CA (p<0.001). Similarly, the functional activity of MRP-1 was significantly increased...
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Posted by admin on Nov 30, 2020 in |
Polyherbal formulation (HF) containing different herbs have been used to treat diabetic patients by ayurvedic practitioners in India. The present study reports the effect of HF alone and with metformin on different rat models of hyperglycemia. HF treatment with HF alone and in combination with metformin elicited a significant hypoglycemic effect on epinephrine hyperglycemia and alloxan diabetic rats. HF treatment also altered the glucose-tolerance curve pattern both in normal and diabetic rats. Further, HF treatment alone and with metformin increased liver glycogen, glucose transfer in the liver, enhanced glucose uptake process in peripheral muscles, and also improved architecture of β-islets of Langerhans of diabetic rats. The combination of HF with metformin elicited a greater degree of hypoglycemic activity, which was reflected both on blood glucose level as well as other biochemical parameters studied. The hypoglycemic effect of HF may be mediated through pancreatic as well as extrapancreatic systems. Further, HF combination with metformin seems to be useful in diabetes mellitus and needs to be investigated on human subjects to...
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Posted by admin on Nov 30, 2020 in |
An attempt has been made in the present study to isolate and identify actinomycetes, which can produce bioactive metabolites possessing antimicrobial activity. The soil samples were collected from mangrove sediments of Krishna district, Andhra Pradesh. The potent secondary metabolite producing strains were isolated and designated as VLK-15, VLK-24, and VLK-56. Identification of the strains was carried out basing on morphological, cultural, physiological and biochemical characters. Phylogenetic analysis of 16S rRNA gene sequence showed that the strains VLK-15, VLK-24, VLK-56 form distinct clade within the genus Streptomyces. The 16S rRNA sequences were deposited in the GenBank database of NCBI under the accession numbers MG309759 (Streptomyces olivoverticillatus VLK-15), MG309760 (Strepto-myces rubrus VLK-24), and MF952650 (Streptomyces sampsonii-VLK-56). The antimicrobial activity of the strains was evaluated by using agar well diffusion assay. The ethyl acetate extracts were highly effective against Candida albicans, Staphylococcus aureus, Bacillus megaterium followed by Bacillus subtilis, Escherichia coli and Klebsiella...
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Posted by admin on Nov 30, 2020 in |
Penetrating biomolecules by ultrasonic techniques is an innovative and influential tool for characterizing their physicochemical properties. Peptides have been used as extensive applications in drug construction and as an ingredient in nutritional complements. An investigation based on the behavior of promulgation of ultrasonic waves in solids and the liquid system is now rather well recognized as an effective means for scrutinizing certain physical properties of the materials. The data attained from ultrasonic proliferation parameters such as ultrasonic velocity, density, viscosity, etc., and additional variation with concentration of one of the medium modules are useful in consideration of the nature of molecular interaction in terms of physical parameters. Owing to the sensitivity to very low populace densities at high energy circumstances, ultrasonic methods have been preferred and are testified to be corresponding to other techniques. An exhaustive study of literature exposes that the ternary solutions and liquid mixtures were premeditated and reported by different inventors. They testified on the nature of forces elaborate in the formation of the hydrogen bonds....
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Posted by admin on Nov 30, 2020 in |
The aim of this study was to screen the presence of bioactive compounds through GC-MS analysis and carry out the ant-inflammatory test of the methanolic tissue extract of Murex tribulus, a spiny predatory gastropod that is widespread from the Central Indian Ocean to Western Pacific Ocean. The GC-MS analysis of the methanolic flesh extract revealed the presence of four bioactive compounds, 1, 2 dimethyl hydrazine, urea, cis-9 hexadecenoic acid, and 1, 1, 1, 3, 5, 5, 5 hepta-methyltrisiloxane. Among the compounds identified, 1, 1, 1, 3, 5, 5, 5-Heptamethyltrisiloxane was the most abundant antimicrobial compound (92.87%) present in the methanolic tissue extract of M. tribulus. The results of the GC-MS analysis showed that the bioactive compounds present in the tissue extract showed anti-inflammatory responses. Hence in-vitro anti-inflammatory activity in the tissue extract of M. tribulus was determined by proteinase inhibitory activity and 5-lipooxygenase inhibitory assay. The anti-inflammatory test showed promising results with high percentage of inhibition such as 53.50% at a concentration of 1000 µg/ml in proteinase inhibitory activity...
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