Posted by admin on Dec 31, 2019 in |
Gmelina arborea Roxb. belongs to the Verbenaceae family, commonly known as Gambhar. It is distributed throughout India, Ceylon, Malayan and Philippine Islands. Its roots have anthelmintic properties. Its flowers are useful in the treatment of leprosy and blood diseases. Its fruit has diuretic properties and aphrodisiac. It is also used in anemia leprosy and vaginal discharges. Leaves have shown analgesic, antimicrobial, and anti-diabetic activities. A new flavone glycosides 5, 7.4ʹ-trihydroxy-8, 3ʹ-dimethoxyflavone- 4ʹ-O-β-D-glucopyranosyl- 7-O-β-D-xylopyranosyl (1→2)-α-L-rhamnopyranoside(A) along with two known compounds Luteolin (B) and Apigenin (C) were isolated from ethanolic extract of the stem parts of the plant. The characterization and structural elucidation of these compounds were carried out by various color reactions, chemical degradation, and spectral analysis. Antioxidant activity of compound A was evaluated by 1, 1-diphenyl-2-pycrylhydrazyl (DPPH) assay system which has shown a significant radical scavenging...
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Posted by admin on Dec 31, 2019 in |
The present study depicts the assessment of class III antiarrhythmic drug dronedarone in its drug substance and drug product. Response surface randomized central composite quadratic design has been employed for the optimization of method parameters using reverse-phase high-performance liquid chromatography (RP-HPLC) on Kromasil C18 250 × 4.6 mm, 5 µ with UV detection at 289 nm. The ranges of the independent variables used for the optimization were flow rate 0.9 to 1.1, column temperature 28 °C to 33 °C and composition of buffer in the mobile phase is 55 to 65%. The influence of these independent variables on the output responses: retention time, asymmetric factor, theoretical plates, plate height and capacity factor were evaluated. The five responses were simultaneous optimized by using central composite design. Optimum conditions chosen for the assay were flow rate of 0.945 ml/min, temperature 31.3 °C and buffer: Acetonitrile has taken in the ratio 61.93: 38.07 respectively. The retention time of dronedarone is 2.356 minutes with the employment of the optimum conditions given by the...
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Posted by admin on Dec 31, 2019 in |
Sarecycline, a narrow spectrum tetracycline-derived antibiotic, is used to treat moderate to severe Acne vulgaris. The current study is aimed at developing a simple, sensitive and accurate liquid chromatography-tandem mass spectrometric (LC/MS/MS) method and validating the same for determination of sarecycline in human plasma. Zorbax, SB C18, 4.6 mm × 75 mm, 3.5 μm, 80 Å column, 10 mM ammonium formate (pH 4.5.): acetonitrile (50:50 v/v) mobile phase was used for chromatographic separation of sarecycline. The sarecycline and minocycline were monitored by electrospray ionization in positive ion multiple reaction monitoring (MRM) modes. This mode was used to detect the sarecycline and minocycline (IS) at m/z 488.6 / 291.1 and 458.4 / 337.2. Liquid-liquid extraction was employed in the extraction of analytes from human plasma. This method is validated over a linear concentration range of 0.5-150.0 ng/mL with a correlation coefficient (r) of ≥ 0.9997. Both drug and internal standards (IS) were stable in plasma...
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Posted by admin on Dec 31, 2019 in |
A selective, accurate, HPLC method was developed by this study for the determination of rifabutin in bulk and capsule dosage form. This method was developed by SHIMADZU LC-2010 HT using C18 column in solvents methanol: acetonitrile: ammonium acetate buffer (50: 45: 05) as mobile phase. At 1.0 ml/min flow rate the mobile phase was pumped, and the sample was detected at 278 nm. For standard rifabutin the retention time was 4.8 min. The method was validated for analytical standards such as linearity, accuracy, precision, and robustness. In a wide range of 5-25 (µg/ml) the linearity was observed. The method was validated, and a recovery study indicates accuracy of this...
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Posted by admin on Dec 31, 2019 in |
Secondary metabolites not only play a vital role in plant defense but also had shown various medicinal properties which provide a scientific base for using herbs and different plants as alternative medicines that were used by ancient communities. Many of the drugs today are simple synthetic modifications of the natural compounds. The developing commercial requirements of secondary metabolites in recent years has resulted in a great interest in secondary metabolism. Different strategies have been studied with the objective of improving the production of secondary metabolites. Many studies proved the role of plant growth regulators in the accumulation and production of many of these active and therapeutic compounds. Short term experiment was carried out to investigate the response of Catharanthus roseus L. plant to the foliar spray of GA3, IAA and 6-bezylaminopurin at (0, 30, 60, 90 ppm) concentrations during the blooming stage. Three parameters were studied, phenols content, alkaloids content and the antioxidant activity in leaves extracts of this plant. Results showed there were significant increments in all studied...
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