Posted by admin on Apr 30, 2020 in |
The aim of the present study is to develop a stability-indicating reverse-phase high-performance liquid chromatography method for the simultaneous estimation of Aspirin and Dipyridamole in combined extended-release dosage form 25/200mg, using a single unit of the capsule. Materials and Methods: Chromatographic separation was achieved with Agilent’s high-performance liquid chromatography and X bridge C8 column, with the mobile phase containing a mixture of ammonium dihydrogen phosphate buffer (pH 2.5): methanol (550:450, v/v) by isocratic elution technique. The flow rate was maintained at 1.2 ml/min, and the detection wavelength was 230nm. Results: Aspirin and Dipyridamole were eluted at 4.7min and 12.6min, respectively, using the developed method. The method was linear in the range of 12.5-50μg/ml for Aspirin and 100-400 μg/ml for Dipyridamole, with an r2 value of 0.9997 and 0.9999, respectively. The sample recoveries observed were 97.63-100.96% and 97.38-98.01%, respectively, for aspirin and dipyridamole. The forced degradation studies were carried out, and the stressed samples were analyzed using the developed method. The purity angle of the peak was observed lesser than the...
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Posted by admin on Apr 30, 2020 in |
Alzheimer’s disease (AD) is a major type of dementia. AD is associated with memory impairment and cognitive deficit. It is characterized by low levels of acetylcholine in the brain of AD patients. The inhibition of acetylcholinesterase (AChE) has been one of the most used strategies for the management of mild to moderate AD. In the present study, n-hexane, ethyl acetate, and hydroalcoholic leaf extracts of two medicinal plants Phyllanthus emblica (Euphorbiaceae) and Tamarindus indica (Fabaceae) were screened for anticholinesterase activity and compared with standard acetyl-cholinesterase inhibitor (galantamine) by Ellman’s spectrophotometric method. The results obtained from the three extracts of P. emblica and T. indica against AChE enzyme was evaluated for percentage inhibition and IC50 values. Among the extracts of P. emblica, ethyl acetate extract (96.62 ± 0.165) at a concentration of 200 µg/ml was found to be potent. Ethyl acetate extract of T. indica showed very potent inhibition (97.55 ± 0.087) at the concentration of 200 µg/ml and found to be equally potent with that of galantamine (98.63 ±...
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Posted by admin on Apr 30, 2020 in |
Plants form the basis of human and animal life. Plants are better choice for medicinal applications when compared to synthetic chemicals and the nature has provided various types of medicinal plants. Jasminum grandiflorum Linn. (family Oleaceae) is a night bloomy flowering plant and is an important source of methyl jasmonates which find utility in plant defense, fruit ripening, plant growth senescence and other physical processes. The aroma plant Jasminum grandiflorum Linn. is native to tropical and warm temperate regions and the plant is observed to have favorable properties which can be used to treat numerous ailments. The leaves of the plant find clinical use in Ayurveda for wound management. The flowers of the plant are used to adorn the women coiffure. In this article, an attempt has been made to provide an updated review on this plant with focus on the isolation and quantification of chemical constituents, medicinal potential and patents on the medicinal and cosmetic formulations comprising Jasminum grandiflorum...
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Posted by admin on Apr 30, 2020 in |
The RP-HPLC stability-indicating assay method has been developed and validated for the estimation of the amlodipine and chlorthalidone in bulk and combined dosage form. The method was optimized by using the mobile phase as a mixture of 0.1% formic acid: methanol: acetonitrile in the ratio of (50:5:45 v/v) at pH 3 was adjusted with orthophosphoric acid. The method was carried out on the Octadecylsilane C18 column (5 µm, 25 cm × 4.6 mm) using a flow rate of 1.0 ml per min. The method was scanned at λmax 266 nm for both the drugs using a PDA detector. The retention time was found to be at 6.32 min and 5.32 min for AML and CHL respectively. The calibration curve determined at respective retention time is found to be 2.5-7.5 μg/ml and 06-18 μg/ml with a regression coefficient of 0.9990 and 0.9940 for AML and CHL respectively. The developed and validated method is reliable, simple, precise and accurate and easy to apply in the...
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Posted by admin on Apr 30, 2020 in |
Cognitive functions are mainly categorized into memory, attention, creativity, and intelligence. Cognitive enhancement may be defined as the amplification or extension of core capacities of the mind. Phenylethylamine is an organic compound which is also known as β-phenylethylamine (β-PEA) and is the name of a class of chemicals which shows psychoactive and stimulant effects. It is a trace amine and a natural monoamine alkaloid. PEA plays an important role in weight loss, treating depression, ADHD and Alzheimer’s disease, etc. The main objective of this study is to synthesize derivatives of ethylamine and phenyl-ethylamine, which may inhibit MAO receptors and improve cognitive behavior. All synthesized derivatives were studied for their biological evaluation on the brain, and they showed the good neurological activity and found effective to reverse the memory deficits induced by scopolamine. While, there is no significant effect was seen in the locomotor effect, which represent that these agents are effective to control the motor...
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